Surveillance and Characterization of Enterotoxigenic Staphylococci in Foods of Animal Origin Collected in the Veneto Region

Manfreda, Gerardo; Mioni, R.; Cesare, Alessandra De

doi:10.1007/s11259-005-0074-5

Cited by 9 publications

(12 citation statements)

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“…Ten different ribotype patterns were observed among 18 S. aureus isolates in a study by Salmenlinna et al (2000) by using HindIII restriction endonuclease. The existence of high molecular diversity of the bacterium has also been demonstrated in other studies (Kishimoto et al, 2004;Manfreda et al, 2005;Rodriguez-Calleja et al, 2006). The large number of different ribotype profiles observed in this study and others points to substantial ecological diversity among this bacterium.…”

Section: Resultssupporting

confidence: 86%

“…Hybridization of restriction endonuclease digested chromosomal DNA with rRNA gene probes, termed as ribotyping, is an effective tool to investigate the molecular epidemiology of pathogenic bacteria and also to understand its genetic diversity (Grimont & Grimont, 1986). Ribotyping has been recently used to identify and differentiate S. aureus isolated from food (Manfreda, Mioni, & De Cesare, 2005;Rodriguez-Calleja, Garcia-Lopez, Santos, Otero, & Garcia-Lopez, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Toxigenicity and genetic diversity of Staphylococcus aureus isolated from Vietnamese ready-to-eat foods

et al. 2010

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Section: Resultssupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Toxigenicity and genetic diversity of Staphylococcus aureus isolated from Vietnamese ready-to-eat foods

et al. 2010

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“…The lowest number of outbreaks is associated with SEE. SEC has been recognized as an important source of SFP associated with the consumption of dairy products (Manfreda et al 2005, Tamarapu et al 2001.…”

Section: Characteristics Of Staphylococcal Food Poisoning (Sfp)mentioning

confidence: 99%

Staphylococcus aureus and Staphylococcal enterotoxins in foodborne diseases

Pexara

Bourriel

Govaris

2018

J Hellenic Vet Med Soc

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ABSTRACT. Staphylococcal food poisoning (SFP) is one of the most significant foodborne diseases. It is a mild intoxication, which is caused by the ingestion of food containing one or more preformed staphylococcal enterotoxins (SEs). The toxic dose of SEs in human is usually ranged from 20 ng to 1 μg. SEs are proteins produced by Staphylococcus spp in various parts of the environment, including foods. Although several staphylococci can produce SEs, the majority of SFP cases is attributed to S. aureus. Traditionally, five antigenic SE types have been recognized: SEA, SEB, SEC, SED and SEE. During the 1990's, new SEs (SEG, SEH, SEI and SEJ) were reported and their genes were described. Several studies revealed that SEH, SEG and SEI were, also, involved in the gastroenteric syndrome. More recent data have indicated the presence of "new" SE genes and new SEs, designated as "staphylococcal enterotoxin-like" (SEI). The role of SEI in food poisoning has not yet been clarified. In contrast to S. aureus, SEs are remarkably heat resistant (D-values of 3-8 min at 121 ° C). They may be present in foods even when viable cells of S. aureus are absent. The enterotoxins are, also, resistant to proteolytic enzymes. It is generally accepted that toxic levels of SEs are produced in foods when S. aureus concentration exceeds 10 5 cfu/ml. S. aureus can grow at a temperature range of 7-48.5°C, with optimum 30-37°C, a pH range of 4.2-9.3, with optimum 7-7.5, minimum a w of 0.86, and up to 15% NaCl. SEs can be produced at a temperature range of 10-46 ° C, with optimum 40-45 ° C, a pH range of 4.8-9.0, with optimum 5.3-7.0, and a w range of 0.86-0.99, with optimum 0.90. Among the foods implicated in SFP are usually milk, dairy products and meat. SEA and SED are usually identified in foodborne outbreaks, while SEC is an important cause of SFP associated with the consumption of dairy products. The European regulation has set criteria for presence of SEs in cheeses, milk powder and whey powder (Regulation EC, 2073. If population of coagulase-positive staphylococci in samples exceeds 10 5 cfu/g, these samples should be further tested for the presence of SEs. In this case, SEs must not be detected in 25 g of the products. ΠΕΡΙΛΗΨΗ. Η σταφυλοκοκκική τοξινωση (ΣΤ) είναι ένα από τα πιο συχνά αίτια τροφιμογενοΰς νόσου. Είναι μιατοξίνωση που προκαλείται από την πρόσληψη με τα τρόφιμα μίας ή περισσοτέρων προσχηματισμένων σταφυλοκοκκικών εντεροτοξινών (ΣΕ). Οι τοξικές δόσεις των ΣΕ για τον άνθρωπο κυμαίνονται από 20 ng έως 1 μg. Οι ΣΕ είναι πρωτεΐνες που παράγονται από ορισμένα είδη σταφυλόκοκκων τόσο στο περιβάλλον όσο και στα τρόφιμα. Παρόλο που διάφορα είδη σταφυλόκοκκου μπορούν να προκαλέσουν τροφιμογενείς λοιμώξεις, σχεδόν όλα τα περιστατικά ΣΤ αποδίδονται στο S. aureus. Παραδοσιακά έχουν αναγνωριστεί οι κλασικοί τύποι σταφυλοκοκκικών εντεροτοξινών SEA, SEB, SEC, SED και SEE. Στη δεκαετία του 1990 ανι χνεύτηκαν νέες τοξίνες (SEG, SEH, SEI, SEJ) και προσδιορίστηκαν τα γονίδια που ελέγχουν την παραγωγή τους. Πιο πρόσφατα στοιχεία οδήγησαν στον πρ...

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“…Hence, in the present study, the S. aureus isolates found capable of producing any of these SE can pose public health risks. In particular, the high percentage of SEC-positive S. aureus isolates among other enterotoxigenic types (from both OM and CM) is of great importance because SEC has been recognized as an important cause of SFP associated with the consumption of dairy products (Manfreda et al 2005). …”

Section: Detection Of Enterotoxigenic S Aureusmentioning

confidence: 99%

Occurrence and antibiotic resistance of enterotoxigenic Staphylococcus aureus in raw ovine and caprine milk in Greece

Pexara

Σολωμάκος

Sergelidis

et al. 2015

Dairy Sci. & Technol.

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Ovine (n=140) and caprine (n=35) raw bulk tank milk samples from farms in central Greece were examined for the occurrence of enterotoxigenic Staphylococcus aureus. The S. aureus isolates were screened for staphylococcal enterotoxin (SE) production, the presence of enterotoxin genes, antibiotic resistance (AR), and methicillin resistance. S. aureus was isolated from 24.3% and 31.4% of ovine and caprine milk samples, respectively. Among the S. aureus isolates of ovine milk (n=34) and caprine (n=11) milk, the enterotoxigenic (SEA-SED) isolates were 21 (61.8%) and 7 (63.6%) for the ovine and caprine milk, respectively. Most toxigenic isolates harbored more than one toxin gene and a total of 11 distinct toxinotypes were detected. The most frequent toxin-gene combinations were "sec, tst" (8 isolates), "seb, seg, sei, tst" (4), "seb, seg, sei" (3), and "seb" (3). Six isolates displayed multiple AR towards up to five antimicrobials. Among ovine milk isolates, the highest resistance frequency was observed towards erythromycin (11.8% of the isolates) and tetracycline (8.8%). Among caprine milk isolates, the most frequent resistance was observed towards erythromycin (18.2%). One methicillin-resistant S. aureus (MRSA) isolate was detected in an ovine milk sample and belonged to spa type t4038. This spa type

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Surveillance and Characterization of Enterotoxigenic Staphylococci in Foods of Animal Origin Collected in the Veneto Region

Abstract: Manfreda, G.,Mioni, R. and De Cesare A., 2005. Surveillance and characterization of enterotoxigenic Staphylococci in foods of animal origin collected in the veneto region. Veterinary Reasearch Communications, 29(Suppl. 2) 331-333

Cited by 9 publications

References 5 publications

Toxigenicity and genetic diversity of Staphylococcus aureus isolated from Vietnamese ready-to-eat foods

Toxigenicity and genetic diversity of Staphylococcus aureus isolated from Vietnamese ready-to-eat foods

Staphylococcus aureus and Staphylococcal enterotoxins in foodborne diseases

Occurrence and antibiotic resistance of enterotoxigenic Staphylococcus aureus in raw ovine and caprine milk in Greece

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