Surveillance, isolation and complete genome sequence of bovine parainfluenza virus type 3 in Egyptian cattle

Sobhy, Nader M.; Mor, Sunil K.; Bastawecy, Iman M.; Fakhry, Hiam; Youssef, Christiana Rezk Bottros; Goyal, Sagar M.

doi:10.1016/j.ijvsm.2017.02.004

Cited by 12 publications

(11 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Amplified single BPI-3 fragment was sequenced and analyzed with already available BPI-3 sequences at the NCBI database to confirm the disease. It was closely related to the BPI-3 sequences from the USA, Egypt and Japan [49][50][51]. This confirms the circulation BPI-3 in the Indian bovine population.…”

Section: Discussionsupporting

confidence: 72%

Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India

Kamdi

Singh

et al. 2020

Microbial Pathogenesis

View full text Add to dashboard Cite

and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India, Microbial Pathogenesis, https://doi.Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus 1 and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from 2 India 3 Abstract 23 Pneumonia in bovines is a multifactorial disease manifestation leading to heavy 24 economic losses. Infections of bovine respiratory syncytial virus (BRSV) and bovine 25 parainfluenza virus-3 (BPI-3) are among the important contributing factors for the development 26 of pneumonia in young animals. These viral agents either primarily cause pneumonia or 27 predispose animals to the development of pneumonia. Although, the role of BRSV and BPI-3 in 28 the pathogenesis of pneumonia is well established, there are no reports of involvement of BRSV 29 and BPI-3 from Indian cattle and buffaloes suffering from pneumonia. In the present 30 investigation, we performed postmortem examinations of 406 cattle and buffaloes which were 31 below twelve months of age. Out of 406 cases, twelve (2.95%) cases were positive for BRSV 32 and fifteen (3.69%) cases were positive for BPI-3, screened by reverse transcriptase polymerase 33 chain reaction (RT-PCR). Further, positive cases were confirmed by sequence analysis of RT-34 PCR amplicons and direct immunofluorescence antibody test (d-FAT) in paraffin-embedded 35 lung tissue sections. BRSV positive cases revealed characteristic findings of bronchiolar 36 epithelial necrosis, thickened alveolar septa by mononuclear cells infiltration and edema; 37 alveolar lumens were filled with mononuclear cells and numerous syncytial cells were seen 38 having intracytoplasmic inclusions. The BRSV antigen distribution was found to be in 39 bronchiolar and alveolar epithelium and syncytial cells in the lung sections. In fifteen cases 40 where BPI-3 was detected, bronchointerstitial pneumonia in the majority of cases with thickened 41 alveolar septa by mild macrophage infiltration, hyperplasia of type-II pneumocytes and 42 bronchiolar necrosis along with syncytial cells having intracytoplasmic inclusions in the majority 43 of cases were observed. The BPI-3 antigen distribution was found to be in bronchiolar and 44 alveolar epithelium and syncytial cells in the lung sections. RT-PCR amplicons of BRSV and 45 BPI-3 obtained were sequenced and their analysis showed homology with already available 46 sequences in the NCBI database. It is the first report of detection of BRSV and BPI-3 from 47 pneumonic cases by RT-PCR and d-FAT from cattle and buffaloes of India, indicating the need 48 for more epidemiological studies. 49 employment opportunities and provides social and financial security. In the course of 67 development of livestock industry, the health of replacement progeny calves is an important 68 component of total operation profitability as the heifer calf is the foundation of the future herd 69 (Razzaque et al., 2009). H...

show abstract

Section: Discussionsupporting

confidence: 72%

Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India

Kamdi

Singh

et al. 2020

Microbial Pathogenesis

View full text Add to dashboard Cite

show abstract

“…El virus de la PI-3 es uno de los patógenos respiratorios virales más importantes del ganado joven y adulto asociado con el CRB (17) . En el municipio de Montería se ha informado una prevalencia del 13.5 % en bovinos de doble propósito (7) y 41 % en terneros recién nacidos (12) ; en el departamento de Antioquia en la raza criolla Blanco Orejinegro, se reportó una prevalencia de 68.9 % (18) .…”

Section: Resultados Y Discusiónunclassified

Seroprevalencia de agentes virales del Complejo Respiratorio Bovino en razas criollas del Centro de Investigación Turipaná de AGROSAVIA

Ramos

Oviedo-Socarras

Pastrana

et al. 2020

Rev. Mex. Cienc. Pecu.

View full text Add to dashboard Cite

Se realizó un estudio descriptivo de corte transversal para determinar la prevalencia y factores epidemiológicos asociados con las enfermedades virales del Complejo Respiratorio Bovino (CRB) en razas criollas del Centro de Investigación Turipaná - AGROSAVIA (Colombia). Se evaluaron 403 bovinos de la raza Romosinuano y 445 Costeño con Cuernos (CCC). A través de la técnica de ELISA indirecta se determinó la presencia de anticuerpos para diarrea viral bovina (DVB), rinotraqueitis infecciosa bovina (IBR), parainfluenza-3 (PI3) y virus sincitial respiratorio bovino (BRSV). Se obtuvieron las prevalencias y se evaluó la asociación entre los agentes virales y entre estos con las variables sexo, edad, tipo de hato y raza; la prueba de Ji-cuadrada se aplicó con un nivel de significancia del 5% y el efecto de la asociación fue determinado por la razón de probabilidades (OR). se constituyó un modelo de regresión logística para explicar la enfermedad más prevalente. Las prevalencias medias en las dos razas fueron: DVB (33.02 %), BRSV (18.51 %), IBR (12.85 %) y PI3 (11.20 %); sin embargo, individualmente, la raza CCC presentó mayor prevalencia para todas las enfermedades. El modelo de regresión mostró una asociación entre DVB, IBR y PI3, las variables sexo, edad, hembras con más de 1 año de edad y la raza CCC. Para abordar las enfermedades del CRB, se recomiendan acciones con énfasis en el control y prevención de DVB y estudios más profundos para entender la dinámica y co-endemicidad de la DVB, IBR, BRSV y PI3 en las razas estudiadas.

show abstract

“…To date, based on phylogenetic analysis, three known BPIV-3 genotypes, designated A(BPIV-3a), B(BPIV-3b) and C(BPIV-3c), have been described [2,6]. Genotype A was first isolated in the United States, but has also been isolated in Egypt, China and Japan [6,7]. Genotype B was first reported in Australia [4].…”

Section: Introductionmentioning

confidence: 99%

Characterisation of the First Bovine Parainfluenza Virus 3 Isolate Detected in Cattle in Turkey

Albayrak

Yazıcı

Özan

et al. 2019

Veterinary Sciences

View full text Add to dashboard Cite

A respiratory disease outbreak on a cattle farm in northern Turkey produced respiratory tract symptoms and severe pneumonia symptoms in 20 calves. Eight calves died, and a lung specimen from one carcass was analysed for bacteria and for viruses of the Bovine respiratory diseases complex. Bacteriological analysis was negative, but antigen detection ELISA and RT-PCR results indicated the presence of Bovine parainfluenza virus (BPIV). Virus isolation succeeded on Madin-Darby Bovine Kidney cells, and subsequent whole genome sequencing and phylogenetic analysis identified BPIV-3c. This is the first report of BPIV-3c isolation from cattle in Turkey, indicating the need for more virological and epidemiological studies.

show abstract

Surveillance, isolation and complete genome sequence of bovine parainfluenza virus type 3 in Egyptian cattle

Cited by 12 publications

References 23 publications

Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India

Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India

Seroprevalencia de agentes virales del Complejo Respiratorio Bovino en razas criollas del Centro de Investigación Turipaná de AGROSAVIA

Characterisation of the First Bovine Parainfluenza Virus 3 Isolate Detected in Cattle in Turkey

Contact Info

Product

Resources

About