Surveillance of antimicrobial resistance among Escherichia coli from chicken and swine, China, 2008–2015

Zhang, Peng; Shen, Zhangqi; Zhang, Chunping; Song, Li; Wang, Bing; Shang, Jun; Yue, Xiuying; Qu, Zhina; XinNan, Li; Wu, Liqin; Zheng, Yongjun; Aditya, Anand; Wang, Yang; Xu, Shaofa; Wu, Congming

doi:10.1016/j.vetmic.2017.02.008

Cited by 75 publications

(80 citation statements)

References 19 publications

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“…In both developed and developing countries, antibiotic resistance has been recognized as a problem in the field of human and veterinary medicine (Bottacini et al, 2018;Zhang et al, 2017). There is ample evidence that the widespread use of antibiotics in agriculture and medicine is the main reason for the high resistance rate of Gram-negative bacteria (Bothyna & Randa, 2018).…”

Section: E Coli Isolated From Retail Foodsmentioning

confidence: 99%

Antimicrobial resistance of Escherichia coli isolated from retail foods in northern Xinjiang, China

Zhang

Luo

et al. 2020

Food Science & Nutrition

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To determine antimicrobial resistance, 431 samples of retail foods purchased at different supermarkets in Northern Xinjiang were examined in this study. There were 112 Escherichia coli strains that were isolated, with approximately 26% of the samples contaminated by E. coli. The detection rate of E. coli isolated from pork was the highest (59.6%), followed by mutton (52.6%), retail fresh milk (52.4%), duck (36.4%), beef (35.3%), chicken (33.3%), and ready-to-eat food (12.9%); the E. coli detection rate for fish and vegetables was <11%. The result showed that the 112 isolates were mostly resistant to tetracycline (52%), followed by ampicillin (42%), compound trimethoprim/sulfamethoxazole (37%), amoxicillin (33%), and nalidixic acid (32%), imipenem resistance was not detected. One hundred isolates carried at least one antimicrobial resistance gene. The detection rate of resistance genes of our study was as follows:tetA (38%), tetB (27%), bla OXA (40%), bla TEM (20%), floR (20%), sul1 (16%), sul2 (27%), aad Ala (19%), aadB (11%), strA (28%), and strB (24%); tetC and bla PSE were not detected. Virulence genes fimC, agg, stx2, fimA, fyuA, papA, stx1, and eaeA were found in 52, 34, 21, 19, 6, 3, 2, and 2 isolates, respectively; papC was not detected. There was a statistically significant association between fimC and resistance to ciprofloxacin (p = .001), gentamicin (p = .001), amikacin (p = .001), levofloxacin (p = .001), and streptomycin (p = .001); between fimA and resistance to tetracycline (p = .001), ampicillin (p = .001), compound trimethoprim/sulfamethoxazole (p = .001), and amoxicillin (p = .003); between agg and resistance to gentamicin (p = .001), tetracycline (p = .001), ciprofloxacin (p = .017), and levofloxacin (p = .001); and between stx2 and resistance to ampicillin (p = .001), tetracycline (p = .001), compound trimethoprim/ sulfamethoxazole (p = .002), and amoxicillin (p = .015).

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Section: E Coli Isolated From Retail Foodsmentioning

confidence: 99%

Antimicrobial resistance of Escherichia coli isolated from retail foods in northern Xinjiang, China

Zhang

Luo

et al. 2020

Food Science & Nutrition

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“…Currently, E. coli is known as an important reservoir of resistance genes to critically important antimicrobials in human medicine, including ESC, fluoroquinolones, aminoglycosides and trimethoprim sulfamethoxazole (Pitout, 2012). In a longitudinal survey from 1993 to 2013, an increase in APEC resistance to 14 tested antimicrobial drugs in China was observed (Zhang et al, 2017). Similar increases in resistance trends in APEC over eight years (2002 to 2010) were reported in the USA (Tadesse et al, 2012).…”

Section: Antimicrobial Resistance Trend In E Coli From Poultrymentioning

confidence: 71%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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“…As a result, pig morbidity and mortality greatly reduced, and farmers achieved good economic benefits. However, at present, antibiotic resistance among clinical pathogens isolated from pig farms is reportedly increasing [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…Escherichia coli can also co-infect pigs along with other bacteria or viruses, leading to increased morbidity and mortality in these animals. Some evidence has suggested that E. coli has become increasingly resistant to amphenicols, such as CAP [1,12]. Mutant strains of E. coli can produce enzymes that degrade or inhibit the activity of the antimicrobial agents, modify the antibiotic target sites, reduce the permeability of the membrane to the drug or alter the metabolic pathway or metabolic state, thereby gaining resistance to CAP [13].…”

Section: Introductionmentioning

confidence: 99%

“…Escherichia coli can also acquire resistance genes from other microorganisms or environments, such as CAP-resistant cmlA gene cassettes and R plasmids [14,15]. In previous reports from the 1990s, E. coli isolates from pigs showed varying degrees of resistance to commonly used veterinary antibiotics in China, such as CAP, aminoglycosides and sulfonamides [1,16].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The prevalence of amphenicol resistance in Escherichia coli isolated from pigs in mainland China from 2000 to 2018: A systematic review and meta-analysis

Wang

Cui

et al. 2020

PLoS ONE

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BackgroundAmphenicols have been widely used in the pig industry in China, leading to varying degrees of drug resistance. MethodsThe systematic review was performed according to PRISMA (Preferred Reported Items for Systematic Reviews and Meta-Analysis) recommendations on studies investigating the prevalence of amphenicol-resistant Escherichia coli (E. coli) isolated from pig in mainland China from 2000 to 2018, a random-effects model was selected, then followed by meta-analysis. ResultsA total of 103 articles were included in the study. The results of the meta-analysis revealed that the pooled summarized prevalence of resistance to chloramphenicol (CAP) was 72.31% (95% confidence interval (95% CI) = 67.12%-77.23%) and to florfenicol (FF) was 58.64% (95% CI = 52.48%-64.67%). During the past 18 years, the resistance rate to CAP remained high initially but then declined rapidly after 2012, whereas the resistance rate to FF plateaued (54.13%-59.60%) from 2000-2018. In different parts of China, the rate of resistance to amphenicols among E. coli isolates was fairly consistent, with the exception of the north and northwest regions.

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Surveillance of antimicrobial resistance among Escherichia coli from chicken and swine, China, 2008–2015

Cited by 75 publications

References 19 publications

Antimicrobial resistance of Escherichia coli isolated from retail foods in northern Xinjiang, China

Antimicrobial resistance of Escherichia coli isolated from retail foods in northern Xinjiang, China

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

The prevalence of amphenicol resistance in Escherichia coli isolated from pigs in mainland China from 2000 to 2018: A systematic review and meta-analysis

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