Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city

Carramaschi, Isabel Nogueira; Lopes, Jonathan Christian Oliveira; Leite, Jéssica Albuquerque; Carneiro, Marcos Tavares; Barbosa, Rodrigo Rocha; Boas, Maria Helena Villas; Rangel, Karyne; Chagas, Thiago Pavoni Gomes; Queiroz, Margareth Mc; Zahner, Viviane

doi:10.1016/j.actatropica.2021.105962

Cited by 8 publications

(6 citation statements)

References 44 publications

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“…Following an early study in 1983, in which nalidixic-resistant Campylobacter was reported in flies in Norway [70], there was little interest until 2005. Thereafter there have been numerous reports on a variety of bacteria and resistance genes in flies from Libya [19], North America [24,[71][72][73][74], Morocco [75], Taiwan [26], Japan [76], the Netherlands [77], Spain [78], Zambia [79], Germany [80,81], Brazil [82,83], Bangladesh [84], Ethiopia [85], Thailand [68,86], Nigeria [69], and India [81] (Table 1). Flies were collected in multiple places, including hospitals, streets, abattoirs, poultry farms, cattle farms, pig farms, fish, and fast-food restaurants.…”

Section: Potential Of Flies To Be Sentinels For Antimicrobial Resistancementioning

confidence: 99%

Flies as Vectors and Potential Sentinels for Bacterial Pathogens and Antimicrobial Resistance: A Review

Yin

Kelly

Wang

2022

Veterinary Sciences

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The unique biology of flies and their omnipresence in the environment of people and animals makes them ideal candidates to be important vectors of antimicrobial resistance genes. Consequently, there has been increasing research on the bacteria and antimicrobial resistance genes that are carried by flies and their role in the spread of resistance. In this review, we describe the current knowledge on the transmission of bacterial pathogens and antimicrobial resistance genes by flies, and the roles flies might play in the maintenance, transmission, and surveillance of antimicrobial resistance.

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Section: Potential Of Flies To Be Sentinels For Antimicrobial Resistancementioning

confidence: 99%

Flies as Vectors and Potential Sentinels for Bacterial Pathogens and Antimicrobial Resistance: A Review

Yin

Kelly

Wang

2022

Veterinary Sciences

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“…2009; Carramaschi et al . 2021). Enterococcus species represented the most frequent genus of microorganisms identified among the Diptera sampled, of which 80% of the isolates belonged to the E. faecalis and E. faecium species.…”

Section: Resultsmentioning

confidence: 99%

“…The environmental pathogens are transmitted to the mammary gland through the soil, water, vegetation, organic matter, animal bedding and contaminated utensils and are commonly associated with clinical cases of short duration, occasionally with high severity (Schukken et al 2012) Enterococcus species are found in the gastrointestinal tract of humans and animals and have revealed opportunistic behaviour in animals and human infections. They are usually refractory to conventional antimicrobial therapy (Gao et al 2020), including species isolated from dipterans (Akhtar et al 2009;Carramaschi et al 2021). Enterococcus species represented the most frequent genus of microorganisms identified among the Diptera sampled, of which 80% of the isolates belonged to the E. faecalis and E. faecium species.…”

Section: Environmentalmentioning

confidence: 99%

Prevalence of bovine mastitis-related pathogens, identified by mass spectrometry in flies (Insecta, Diptera) captured in the milking environment

Bertolini

Prado

Thyssen

et al. 2022

Letters in Applied Microbiology

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Significance and Impact of the Study: The high diversity of Diptera (Insecta) species (>157 000) worldwide, feeding habits, and the access to contaminated environments, make these insects potential vector-borne of more than 200 pathogens to humans and animals. Here, 217 adult specimens of Diptera were collected in the environment of nine dairy farms with a history of clinical mastitis. From these, 275 microorganisms were identified, with a predominance of pathogens related to environmental bovine mastitis (Enterococcus spp. and Escherichia coli), in addition to agents with well-known zoonotic nature (e.g. Bacillus cereus), indicating that control of this insect in dairy farms pose relevance in human and animal health.

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“…According to the literature, flies, from the Muscidae and Calliphoridae families are the most frequent in hospitals, farms, and aviaries (Akter et al, 2020;Carramaschi et al, 2021;Evers, Blaak, Hamidjaja, de Jonge, & Schets, 2016). The use of antimicrobials in these places is frequent, thus, the habits of these insects to travel through different environments, to regurgitate and defecate on food and surfaces, in addition to having a body full of numerous bristles and always very close to man, increases the risk of contamination by antibiotics bacteria resistant (Evers et al, 2016;Stoffolano, 2019).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural and pathogenicity of Brevibacillus laterosporus against sinantropic muscoid dipterans

Pereira¹,

Queiroz

Faria

et al. 2021

Microscopy Res & Technique

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Brevibacillus laterosporus has entomopathogenic potential against several orders of insects and its wide bioactivity is associated with a variety of strain-specific molecules.In order to avoid the use of synthetic insecticides, along with the need to control insect pests, microbial control has been widely used. Muscoid dipterans are known for their medical-veterinary and sanitary importance, and synanthropy. The enormous biotechnological potential of B. laterosporus has been demonstrated, but there are still few studies with muscoid dipterans. The aim of the study was to verify the mortality of B. laterosporus NRS590 on synanthropic flies and to characterize its different cell stages ultrastructurally. The flies were collected from garbage bins and the colonies were adapted to the laboratory conditions. Bioassays with neo larvae were carried out from the bacterial growth in the phases: vegetative (6 hr), sporangium (20 hr), and free spores (44 hr). An aliquot of each phase was collected for Transmission (TEM) and Scanning Electron Microscopy (SEM). The effectiveness of NRS590 was observed in the sporulation phase, where the corrected mortality was 83.3, 85.1, and 99% for Chrysomya megacephala, Chrysomya putoria, and Musca domestica, respectively. The parasporal body was observed in detail on the entire spore surface. Although our knowledge of this bacterium is growing, it remains to be determined the real virulence factors responsible for the wide entomopathogenic activity observed on muscoid dipterans. Therefore, this study can provide subsidies for the improvement of efficient and safe microbial control techniques for the environment and living beings.

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Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city

Cited by 8 publications

References 44 publications

Flies as Vectors and Potential Sentinels for Bacterial Pathogens and Antimicrobial Resistance: A Review

Flies as Vectors and Potential Sentinels for Bacterial Pathogens and Antimicrobial Resistance: A Review

Prevalence of bovine mastitis-related pathogens, identified by mass spectrometry in flies (Insecta, Diptera) captured in the milking environment

Ultrastructural and pathogenicity of Brevibacillus laterosporus against sinantropic muscoid dipterans

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