Surveillance of Zika virus in field-caught Aedes aegypti and Aedes albopictus suggests important role of male mosquitoes in viral populations maintenance in Medellín, Colombia

Calle-Tobón, Arley; Pérez-Pérez, J.R.; Rojo, Raúl; Rojas-Montoya, Winston; Triana-Chávez, Omar; Rúa‐Uribe, Guillermo; Gómez-Palacio, Andrés

doi:10.1016/j.meegid.2020.104434

Cited by 12 publications

(15 citation statements)

References 23 publications

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“…Natural infection of Ae. albopictus with dengue and Zika viruses has already been reported [ 9 , 42 , 44 ]; however, its role as an arbovirus vector in the country should be further clarified, especially considering the results in our study. The low amount of Ae.…”

Section: Discussionmentioning

confidence: 65%

“…aegypti is the main vector in Medellín, the continuous increase of Ae. albopictus abundancy, its presences across at least 70% of the city neighborhoods [ 41 ], and the possibility this species may not be well controlled contribute to increase its role as a vector, not only in Medellín, but also in other cities where it is distributed [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

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Entomovirological Surveillance in Schools: Are They a Source for Arboviral Diseases Transmission?

Pérez-Pérez

Peña-García

Calle-Tobón

et al. 2021

IJERPH

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Surveillance and control activities for virus-transmitting mosquitoes have primarily focused on dwellings. There is little information about viral circulation in heavily trafficked places such as schools. We collected and analyzed data to assess the presence and prevalence of dengue, chikungunya, and Zika viruses in mosquitoes, and measured Aedes indices in schools in Medellín (Colombia) between 2016–2018. In 43.27% of 2632 visits we collected Aedes adults, creating 883 pools analyzed by RT-PCR. 14.27% of pools yielded positive for dengue or Zika (infection rates of 1.75–296.29 for Aedes aegypti). Ae. aegypti was more abundant and had a higher infection rate for all studied diseases. Aedes indices varied over time. There was no association between Aedes abundance and mosquito infection rates, but the latter did correlate with cases of arboviral disease and climate. Results suggest schools are important sources of arbovirus and health agencies should include these sites in surveillance programs; it is essential to know the source for arboviral diseases transmission and the identification of the most population groups exposed to these diseases to research and developing new strategies.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Entomovirological Surveillance in Schools: Are They a Source for Arboviral Diseases Transmission?

Pérez-Pérez

Peña-García

Calle-Tobón

et al. 2021

IJERPH

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“…aegypti and Ae . albopictus are infected with DENV and ZIKV [ 9 – 11 ]. Since 2000, the number of dengue cases in Medellín has increased continuously, with periodic outbreaks, and nearly 20,000 dengue cases have been reported in each of the last major outbreaks occurring in 2010 and 2016 [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Local-scale virome depiction in Medellín, Colombia, supports significant differences between Aedes aegypti and Aedes albopictus

et al. 2022

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Aedes spp. comprise the primary group of mosquitoes that transmit arboviruses such as dengue, Zika, and chikungunya viruses to humans, and thus these insects pose a significant burden on public health worldwide. Advancements in next-generation sequencing and metagenomics have expanded our knowledge on the richness of RNA viruses harbored by arthropods such as Ae. aegypti and Ae. albopictus. Increasing evidence suggests that vector competence can be modified by the microbiome (comprising both bacteriome and virome) of mosquitoes present in endemic zones. Using an RNA-seq-based metataxonomic approach, this study determined the virome structure, Wolbachia presence and mitochondrial diversity of field-caught Ae. aegypti and Ae. albopictus mosquitoes in Medellín, Colombia, a municipality with a high incidence of mosquito-transmitted arboviruses. The two species are sympatric, but their core viromes differed considerably in richness, diversity, and abundance; although the community of viral species identified was large and complex, the viromes were dominated by few virus species. BLAST searches of assembled contigs suggested that at least 17 virus species (16 of which are insect-specific viruses [ISVs]) infect the Ae. aegypti population. Dengue virus 3 was detected in one sample and it was the only pathogenic virus detected. In Ae. albopictus, up to 11 ISVs and one plant virus were detected. Therefore, the virome composition appears to be species-specific. The bacterial endosymbiont Wolbachia was identified in all Ae. albopictus samples and in some Ae. aegypti samples collected after 2017. The presence of Wolbachia sp. in Ae. aegypti was not related to significant changes in the richness, diversity, or abundance of this mosquito’s virome, although it was related to an increase in the abundance of Aedes aegypti To virus 2 (Metaviridae). The mitochondrial diversity of these mosquitoes suggested that the Ae. aegypti population underwent a change that started in the second half of 2017, which coincides with the release of Wolbachia-infected mosquitoes in Medellín, indicating that the population of wMel-infected mosquitoes released has introduced new alleles into the wild Ae. aegypti population of Medellín. However, additional studies are required on the dispersal speed and intergenerational stability of wMel in Medellín and nearby areas as well as on the introgression of genetic variants in the native mosquito population.

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“…aegypti and Ae. albopictus are infected with DENV and ZIKV [9][10][11]. Since 2000, the number of dengue cases in Medellín has increased continuously, with periodic outbreaks, and nearly 20,000 dengue cases have been reported in each of the last major outbreaks occurring in 2010 and 2016 [12,13].…”

mentioning

confidence: 99%

“…aegypti, but Ae. albopictus may potentially be a secondary vector as it can be naturally infected with DENV2 [10,32] and ZIKV [11,33]. Both Medellín and its adjacent city, Bello, sustain arboviruses at consistent rates.…”

mentioning

confidence: 99%

Local-scale virome depiction supports significant differences between Aedes aegypti and Aedes albopictus

Calle-Tobón

Pérez-Pérez

Forero-Pineda

et al. 2022

Preprint

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Aedes spp. comprise the primary group of mosquitoes that transmit arboviruses such as dengue, Zika, and chikungunya viruses to humans, and thus these insects pose a significant burden on public health worldwide. Advancements in next-generation sequencing and metagenomics have expanded our knowledge on the richness of RNA viruses harbored by arthropods such as Ae. aegypti and Ae. albopictus ; increasing evidence suggests that vectorial competence can be modified by the microbiome (comprising both bacteriome and virome) of mosquitoes present in endemic zones. Using an RNA-seq-based metataxonomic approach, this study determined the virome structure of field-caught Ae. aegypti and Ae. albopictus mosquitoes in Medellín, Colombia, a municipality with a high incidence of mosquito-transmitted arboviruses. The two species are sympatric, but their core viromes differed considerably in richness, diversity, and abundance; the viromes were dominated by a few viruses. BLAST searches of assembled contigs suggested that at least 17 virus species (16 of which are insect-specific viruses [ISVs]) infect the Ae. aegypti population. Dengue virus 3 was detected in one sample. In Ae. albopictus , up to 11 ISVs and one plant virus were detected. Therefore, the virome composition was species-specific. The bacterial endosymbiont Wolbachia was identified in all Ae. albopictus samples and in some Ae. aegypti samples collected after 2017. The presence of Wolbachi a sp. in Ae. aegypti was not related to significant changes in the richness, diversity, or abundance of this mosquito’s virome, although it was related to an increase in the abundance of Aedes aegypti To virus 2 (unclassified). The mitochondrial diversity of these mosquitoes suggested that the Ae. aegypti population underwent a change that started in the second half of 2017, which coincides with the release of Wolbachia -infected mosquitoes in Medellín, indicating that the population of w Mel-infected mosquitoes has expanded. However, additional studies are required on the dispersal speed and intergenerational stability of w Mel in Medellín and nearby areas as well as on the introgression of genetic variants in the native mosquito population.

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Surveillance of Zika virus in field-caught Aedes aegypti and Aedes albopictus suggests important role of male mosquitoes in viral populations maintenance in Medellín, Colombia

Cited by 12 publications

References 23 publications

Entomovirological Surveillance in Schools: Are They a Source for Arboviral Diseases Transmission?

Entomovirological Surveillance in Schools: Are They a Source for Arboviral Diseases Transmission?

Local-scale virome depiction in Medellín, Colombia, supports significant differences between Aedes aegypti and Aedes albopictus

Local-scale virome depiction supports significant differences between Aedes aegypti and Aedes albopictus

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