Survey for the presence of Neospora caninum in frozen bull's semen samples by PCR assay

Doosti, Abbas; Khamesipour, Faham; Nekoei, Shahin; Lutvikadić, Ismar

doi:10.1016/s2222-1808(14)60618-6

Cited by 7 publications

(5 citation statements)

References 37 publications

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“…Also, a high infection (57.3%) in North of Iran is related to moderate and rainy climate, which is favorite for sporulation of oocysts (Youssefi et al 2010 ). This rate was 10–95.2% in milk samples (Shakerian et al 2015 ; Binaei 2016 ) and 7.4–10.5% in semen samples (Sharifzadeh et al 2012 ; Doosti et al 2015 ). Bulk milk examination is using routinely for detection of antibodies to N. caninum in dairy farms due to ease of sampling, and the tests are cheap and rapid (Hurkova et al 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Neospora caninum infection in Iran (2004–2020): A review

2020

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Knowledge on neosporosis and associated risk factors in different species of animals are so important for designing the control programs and reduce the economic losses globally. This literature review targeted for evaluating the infection rate of Neospora caninum in animals in Iran. Until April 2020, all of published documents in the main English and Persian-language databases were searched. A total number of 110 documents (English = 85 and Persian = 25) were extracted. Most of reports were seroepidemiological studies using ELISA in Iranian cattle population. The range of Neospora infection was 3.8-76.2% in cattle, 0-54.6% in dogs, 0.9-9.9% in sheep, 6.2% in goats, 19.2-55.9% in buffaloes, 20-42.2% in horses, 52% in donkeys, 3.2-27% in camels, 14% and 19% in cats, and 0-20.4% in rodents. This rate in birds was 17.3% in chicken, 9.8% and 30.4% in pigeons, 2.8% and 3.7% in sparrows, and 9.9% in hooded crows. This is a comprehensive literature review on Neospora infection in Iran at the first time. The infection of N. caninum is widespread in Iran especially in dogs and cattle population. This review can provide baseline information for future research. Study on other hosts especially on wild and exotic animals is recommended for exact estimate of neosporosis in Iran. Investigations into molecular diagnosis and genotyping of N. caninum strains are also needed; this will be helpful for developing vaccines and finding the connection among wild and domestic cycles of disease. Education on the risk factors associated with N. caninum infection for is suggested farmers and rural public. Keywords Neospora caninum Á Neosporosis Á Animal Á Bird Á Risk factor Á Review article Á Iran

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Section: Discussionmentioning

confidence: 99%

Neospora caninum infection in Iran (2004–2020): A review

2020

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“…So, it is essential to test the animals annually or semi-annually especially bulls in AIC centers to monitor the disease status. were recorded by Doosti et al [28] in bovine frozen semen used for artificial insemination.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Buffalo Bull Semen for Some Venereal Diseases Using PCR

Abdel-hameed¹,

Mahmoud²,

Sakr

et al. 2017

NIDOC-ASRT

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A RTIFICIAL INSEMINATION using unaccredited frozen semen is a major approach for diseases transmission. This work intended to scan the buffalo bull semen for Br. melitensis, Toxoplasma gondii and Neospora caninum using Polymerase Chain Reaction (PCR). Fifty bulls were used in this study. Semen was assessed for volume, motility, viability, and chromatin damage. Total genomic DNA was extracted from buffalo semen and amplified by PCR. The results showed that, semen samples were positive to Toxoplasma gondii in 6% of the semen samples, while all the semen samples were totally negative to Br. Melitensis and Neospora caninum. No significant differences were found in semen characteristics between bulls positive or negative to Toxoplasma gondii.In conclusion, the present trial suggests the opportunity of venereal transmission of T. gondii among buffaloes. However, further investigations are required using high number of semen samples from different localities of Egypt.

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“…Studies have reported data on the presence of several pathogens in bovine semen, such as Brucella abortus (Nardi Junior et al, 2014), Mycoplasma bovis (Haapala et al, 2018), Ureaplasma diversum (Marques et al, 2009), Histophilus somnus (Dias et al, 2014), and Neospora caninum (Doosti et al, 2015).…”

Section: Arqmentioning

confidence: 99%

Investigation of Tritrichomonas foetus in cryopreserved bovine semen by culture and polymerase chain reaction

Ribeiro

Silva

Duarte

et al. 2021

Arq. Bras. Med. Vet. Zootec.

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Bovine Trichomoniasis (BT) is an infectious disease caused by Tritrichomonas foetus that can be transmitted either sexually or by fomites. In males, the disease is asymptomatic and permanent. T. foetus has been detected in semen samples where it is able to remain viable even when frozen. The objective of this study was to investigate the presence of T. foetus in 27 samples of commercial frozen bovine semen by culture and Polymerase Chain Reaction (PCR). Samples were thawed in water at 37°C. Part of the samples was inoculated in a test tube containing Diamond’s medium and incubated at 35°C. Growth was evaluated every 24 hours via direct examination under a microscope. The other part was placed in an Eppendorf tube and frozen for later molecular analysis. After 10 days of culture, all samples were negative for T. foetus. The Quick-DNA Miniprep Kit (Zymo Research) without proteinase K was used for DNA extraction. The primers used in PCR were TRF3 and TRF4. PCR results were negative for all samples. In conclusion, bovine semen samples were negative for T. foetus in both diagnostic methods, according to the adopted methodology.

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Survey for the presence of Neospora caninum in frozen bull's semen samples by PCR assay

Cited by 7 publications

References 37 publications

Neospora caninum infection in Iran (2004–2020): A review

Neospora caninum infection in Iran (2004–2020): A review

Evaluation of Buffalo Bull Semen for Some Venereal Diseases Using PCR

Investigation of Tritrichomonas foetus in cryopreserved bovine semen by culture and polymerase chain reaction

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