Survey of Anaplasmataceae bacteria in sheep from Senegal

Abstract

Our studies demonstrated the great variety of pathogenic bacteria from the Anaplasmataceae family in the blood of clinically ill sheep. A. ovis was identified unexpectedly often. For the first time, A. phagocytophilum was found in sub-Saharan Africa, and its further epidemiology may be now reconsidered. The roles of canine pathogen, A. platys, and yet undescribed Anaplasma sp. "Badiouré" in ovine pathology should be more closely studied.

Molecular investigation and phylogeny of Anaplasmataceae species infecting domestic animals and ticks in Corsica, France

“…Seventy-one percent (93/131) of Anaplasmataceae -positive sheep samples were infected by A. ovis . The 23S sequences obtained were identical to each other and showed 100% identity with A. ovis strain KMND Niayes-14 reported in sheep from Senegal [33]. All the other 38 qPCR-positive sheep (29%) were found to be infected by several as yet uncharacterised and potentially new species of Anaplasma .…”

Molecular Survey ofAnaplasmaSpecies in Small Ruminants Reveals the Presence of Novel Strains Closely Related toA. phagocytophilumin Tunisia

“…In sheep, A. ovis prevalence (overall 93.8%, minimum 91.4% in El Alia, maximum 96.4% in Khetmine) was similar to the prevalence reported by de la Fuente et al (2005) in Italy (87%) and by Renneker et al (2013) in Portugal (82.5%). It was higher than that observed in Sudan (41.7%; Renneker et al 2013), Turkey (31.4%;Renneker et al 2013), and Senegal (11.5%;Djiba et al 2013). Overall prevalence in goats (65.3%; range, 44.4-78.8%) was similar to the prevalence reported by Altay et al (2014) in Turkey (66.4%), lower than that estimated in Angola (100%; Kubelová et al 2012), and higher than that observed in China (15.3-25.6%;Liu et al, 2012, Chi et al, 2013.…”

Development of a new PCR-based assay to detect Anaplasmataceae and the first report of Anaplasma phagocytophilum and Anaplasma platys in cattle from Algeria

“…Among those tested, the rpoB and groEL genes were considered too diverse, with few common patterns within the different species of Anaplasmataceae (data not shown). 16S rRNA (rrs) was determined to be not sufficiently diverse for discriminating among closely related species, such as Anaplasma ovis, A. marginale, and A. centrale [12]. The gene encoding the 23S subunit of ribosomal RNA (rrl) was selected for its large size and the presence of, on the one hand, highly conserved regions that enabled developing the family-specific oligonucleotide and, on the other hand, sufficient discrimination between closely related species of Anaplasmataceae.…”

“…The specificity of the Taqman ® assay and the conventional PCR assay was tested using DNA extracted from the following organisms: A. phagocytophilum, A. marginale, A. centrale, A. ovis [12], A. platys (kindly supplied by Pr. L. Chabanne (VetAgro Sup Lyon, France) obtained from the blood of a dog from the Gard department, France), E. canis, E. ruminantium, Neorickettsia sennetsu, Neorickettsia risticii, Rickettsia conorii, Rickettsia felis, Rickettsia typhi, Rickettsia massiliae, Rickettsia raoultii, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, Enterococcus faecalis, Enterococcus faecium, Enterobacter cloacae, Enterobacter aerogenes, Streptococcus agalactiae, Streptococcus oralis, Citrobacter koseri, Haemophilus influenza, Serratia marcescens, Klebsiella oxytoca, Gardnerella vaginalis, Stenotrophomonas maltophilia and human DNA.…”