Survey of bacteria diversity associated with diseased Apostichopus japonicus and bacterial pathologies during the winter

Li, Hua; Qiao, Guo; Gu, J; Zhou, Wei; Li, Qiang; Park, Soo-Il

doi:10.1016/j.jbiotec.2008.07.1423

Cited by 6 publications

(3 citation statements)

References 2 publications

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“…Therefore, winter is the high incidence period of skin ulceration syndrome (SUS), which is the most common disease in sea cucumber (Li et al, 2010). SUS is caused by the infection of pathogenic bacteria such as Vibrio splendidus , which causes skin ulcers and the death of sea cucumber (Deng et al, 2009; Li et al, 2008). During the process of water exchange in culturing, the sea cucumber is prone to body damage and infection, which cause SUS.…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation with Sporosarcina aquimarina MS4 enhances juvenile sea cucumber ( Apostichopus japonicus ) growth, immunity and disease resistance against Vibrio splendidus infection at low temperature

Zhou

Liu

et al. 2021

Aquacult. Nutr.

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Probiotics are important biological control products in the aquaculture of sea cucumber, but probiotics used specifically in low‐temperature environments are rare. The present study used treatment with low‐temperature‐resistant Sporosarcina aquimarina MS4 and investigated changes in growth, immunity, intestinal flora and immune‐related gene expression in sea cucumber at low water temperature (8°C). After 60 days of feeding, S. aquimarina MS4 significantly improved growth performance and immune enzyme activity and reduced mortality in sea cucumbers infected with Vibrio splendidus compared with the commercial diet. The intestinal flora structure of the sea cucumbers fed S. aquimarina MS4 differed from that of the control group. Compared with that of the control group, the intestinal flora of sea cucumbers fed S. aquimarina MS4 was always dominated by Proteobacteria and had a higher abundance of Verrucobacteria, and the flora structure was more stable. The expression of the TLR, MyD88, IRAK4, p38 and NFκB genes was upregulated significantly after the addition of S. aquimarina MS4 to the diet. In summary, low‐temperature‐resistant S. aquimarina MS4 could regulate the intestinal flora and immune‐related gene expression of sea cucumber, thereby improving its immunity and resistance to pathogenic bacteria such as V. splendidus.

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Section: Introductionmentioning

confidence: 99%

Dietary supplementation with Sporosarcina aquimarina MS4 enhances juvenile sea cucumber ( Apostichopus japonicus ) growth, immunity and disease resistance against Vibrio splendidus infection at low temperature

Zhou

Liu

et al. 2021

Aquacult. Nutr.

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“…During the last decade, sea cucumber farming has developed rapidly and become a vigorous industry in northern China Yang et al, 2009). Despite of the rapid expansion and industrialization of sea cucumber farming, this industry has been negatively affected by infectious diseases, mainly skin ulceration syndrome, causing huge economic losses and presenting a major constraint to the expansion of this industry (Wang et al, 2004Wang et al, 2007;Li et al, 2008;Deng et al, 2009). With strict regulations on the use of antibiotics in aquaculture, the development of environmentally friendly approach including pro-, pre-or syn-biotics has been of great interest to confer better health of farmed species.…”

Section: Introductionmentioning

confidence: 99%

Influence of dietary probiotic Bacillus TC22 and Prebiotic fructooligosaccharide on growth, immune responses and disease resistance against Vibrio splendidus infection in sea cucumber Apostichopus japonicus

Zhao

Mai

et al. 2011

J. Ocean Univ. China

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The effects of probiotic Bacillus TC22 (isolated from intestine of infected sea cucumber) and prebiotic fructooligosaccharide (FOS) on growth, immunity and disease resistance in sea cucumber Apostichopus japonicus were studied. Six experimental diets were formulated with combinations of three levels of TC22 (0, 10 7 and 10 9 CFU g −1 diet) and two levels of FOS (0 and 0.5%) in a 3 × 2 factorial experiment. At the end of the 8-week feeding trial, animals were challenged by injecting Vibrio splendidus. The results revealed that the specific growth rates (SGR) of sea cucumbers were not affected by TC22 and FOS, or the interaction between TC22 and FOS (P > 0.05). However, there were significant interactions between TC22 and FOS for immune response and disease resistance in sea cucumbers (P < 0.05). When sea cucumbers were fed with TC22 at 10 9 CFU g −1 feed and 0.5% FOS alone or in combination, the phagocytosis, respiratory burst and phenoloxidase activity of sea cucumber coelomocytes were significantly enhanced; the disease resistance against V. splendidus infection was also increased significantly (P < 0.05). However, dietary combination of TC22 at 10 9 CFU g −1 diet and 0.5% FOS did not yield significantly higher efficiency than either TC22 at 10 9 CFU g −1 diet or 0.5% FOS used alone (P > 0.05). Therefore, further studies should examine the effects of combinations of other levels of FOS (> 0.5% or < 0.5%) and TC22 on the immunity and disease resistance of sea cucumbers.

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“…Sea cucumber, Apostichopus japonicus (Selenka), is one of the most economically important holothurian species, which is the main breed in the north of China. Despite the rapid expansion and industrialization of sea cucumber farming, this industry has been negatively affected by infectious diseases causing huge economic losses and presenting major constraint to the expansion of this industry (Wang et al , , ; Li et al ; Deng et al ). The use of antibiotics has partially solved this problem.…”

mentioning

confidence: 99%

Immunopotentiating Effect of Small Peptides on Primary Culture Coelomocytes of Sea Cucumber, Apostichopus japonicus

Wang

et al. 2015

J World Aquaculture Soc

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The in vitro effect of small peptides on the nonspecific immune response of sea cucumber, Apostichopus japonicus, coelomocytes was studied. Coelomocytes were cultured in L-15 medium containing different concentrations of small peptide (0, 5, 25, and 100 g/mL). Coelomocytes were incubated with the above immunostimulant for 1, 3, 6, 12, and 24 h. Coelomocytes incubated in the medium without immunostimulants were taken as controls at each incubation time. After exposure to small peptides, the coelomocytes were sampled and immune parameters namely, phagocytosis activity, respiratory burst activity, as well as activities of acid phosphatase (ACP), alkaline phosphatase (AKP), and superoxide dismutase (SOD) were determined. All immune parameters were significantly (P < 0.05) enhanced by small peptides. Both phagocytic capacity and respiratory burst activity were up to maximum values at 6 h, and these values were 1.6 and 1.1 times as high as the control group, respectively. Among the humoral responses, the activities of ACP, AKP, and SOD were also up to maximum values at 6 h. The values were 1.4, 1.3, and 1.4 times as high as the control group, respectively. These results suggest that small peptides can enhance the nonspecific immune response of sea cucumber in vitro.

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Survey of bacteria diversity associated with diseased Apostichopus japonicus and bacterial pathologies during the winter

Cited by 6 publications

References 2 publications

Dietary supplementation with Sporosarcina aquimarina MS4 enhances juvenile sea cucumber ( Apostichopus japonicus ) growth, immunity and disease resistance against Vibrio splendidus infection at low temperature

Dietary supplementation with Sporosarcina aquimarina MS4 enhances juvenile sea cucumber ( Apostichopus japonicus ) growth, immunity and disease resistance against Vibrio splendidus infection at low temperature

Influence of dietary probiotic Bacillus TC22 and Prebiotic fructooligosaccharide on growth, immune responses and disease resistance against Vibrio splendidus infection in sea cucumber Apostichopus japonicus

Immunopotentiating Effect of Small Peptides on Primary Culture Coelomocytes of Sea Cucumber, Apostichopus japonicus

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