Survey of Benign Theileria Parasites of Cattle and Buffaloes in Thailand using Allele-Specific Polymerase Chain Reaction of Major Piroplasm Surface Protein Gene.

Abstract

ABSTRACT. During a year from 1999 to 2000, a total of 247 blood samples were collected from 214 cattle and 33 water buffaloes in 16 distinct geographical locations of Thailand and analyzed by allele-specific PCR amplification of major piroplasm surface protein (MPSP) genes of benign Theileria parasites. Four allelic MPSP gene types were determined namely C-type, I-type, B-type and Thai-type, which were originally designated from Japanese Theileria orientalis (Chitose, Ikeda), Australian T. buffeli (Warwick) and Thai T. sp. (Kamphaeng Saen), respectively. Only two allelic MPSP gene types were successively amplified from 204 (82.6%) blood samples. Among positive cases, 138 (67.6%) and 17 (8.3%) samples contained either Thai-type or C-type parasites, respectively, while 49 (24%) s amples contained both types. However, nucleotide sequences of MPSP genes of Thai T. sp. amplified by C-type specific primers revealed higher (96.3%) similarity to Indonesian T. sp. rather than (87.8% similarity) to Japanese T. orientalis (Chitose) designated as C-type. KEY WORDS: benign Theileria, Thailand.

Epidemiological, Clinical and Diagnostic Studies on Blood Parasites in Cattle and Buffaloes in ElBehera Provence

“…But in this study found that T.annulata may be transmitted by Boophilus tick and observed decrease in Hyalomma anatolicum, the proven vector of T. annulata in cattle as shown in table (3). This agree with Sarataphan et al (2003) and Changbunjong et al (2009) who surveyed ticks in cattle in 25 provinces of Thailand and found the cattle tick R.(B) was dominant and had extensive distribution more than 70% were infested with this type of tick.…”

Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

“…They have a cosmopolitan distribution and infect cattle and buffalo in Africa, Australia, Asia, Europe and the United States of America (USA) (Chae et al, 1998;Chansiri et al, 1999;Cossio-Bayugar et al, 2002;Sarataphan et al, 2003;Aktas et al, 2007;Altay et al, 2008;M'ghirbi et al, 2008;Gimenez et al, 2009, Liu et al, 2010a, Wang et al, 2010. The cosmopolitan distribution of these species has been attributed to the global movement of cattle (and buffalo) without any regard to infection, and therefore their distribution mainly depends on the availability of a suitable tick vector (Chae et al, 1999c;Cossio-Bayugar et al, 2002).…”

“…(Allsopp et al, 1994;Chae et al, 1998, Chansiri et al, 1999Gubbels et al, 2000Gubbels et al, , 2002Sarataphan et al, 2003;M'ghirbi et al, 2008;Liu et al, 2010a;Wang et al, 2010;Kamau et al, 2011). We recently identified T .buffeli in some buffalo populations in South Africa (Chaisi et al, 2011).…”

Rapid discrimination and quantification of Theileria orientalis types using ribosomal DNA internal transcribed spacers

“…These types include type1 (Chitose), type2 (Ikeda), type3 (Buffeli) and type-4-8 yet to be classified with taxonomically (Kim et al, 1998;Ota et al, 2009). Mixed infection with different types of T. orientalis in cattle are common in Japan (Kim et al, 2004;Kubota et al, 1995), Korea (Baek et al, 2002) and Thailand (Sarataphan et al, 2003) but their clinical significance remain unclear. According to Shimizu et al, (1992;, at least one to two billion yen (one to two million US$) per year is spent to fight theileria infections in Japan.…”