Survey of citrus tristeza virus (CTV) strains in Citrus x limon (L) Burm f. (lemon) in Tucumán Province, Argentina

Read, David Alan; Palacios, M.F.; Figueroa, Julia; Foguet, Lucas; Kleynhans, Jackie; Stein, Beatriz; Pietersen, Gerhard

doi:10.1007/s10658-017-1245-2

Cited by 4 publications

(4 citation statements)

References 18 publications

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“…In most cases, populations were dominant for RB or Kpg3/SP/T3-like sequences. This supports the previous results of Read and Pietersen (2016) who showed that the distribution of strains within "Star Ruby", as well as a number of other grapefruit cultivars, was often highly heterogeneous, both in South Africa and Argentina (Read et al, 2017). Harper et al (2015) showed that CTV populations tended to reach equilibrium at the same point within clones of plants, regardless of the viral titre in the original inoculum.…”

Section: Results Of Direct Sanger Sequences Of 35 Samples Are Listed Insupporting

confidence: 89%

Survey of Citrus tristeza virus (CTV) on Citrus paradisi (Macfad.) cv. “Marsh” in South Africa

Read

Palacios²,

Figueroa³

et al. 2018

Eur J Plant Pathol

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Citrus tristeza virus (CTV) is endemic throughout the citrus production areas of South Africa and mild-strain cross-protection is employed to combat the negative effects of severe-strain infections. The country is among the world"s leading producers of grapefruit, however crossprotected trees often display severe stem grooving, regardless of the rootstock. Previously, cross-protection sources were evaluated empirically, through graft inoculations onto indicator hosts. Recent research has shown that cross-protection, possibly mediated by super-infection exclusion, is strain-specific. This, coupled with the varying affinities that cultivars display for different CTV strains, has shown the need to determine which CTV strains are associated with specific commercial cultivars. In this study, thirty-six Citrus paradisi (Macfad.) cv. "Marsh" plants were sampled in the South African grapefruit production areas of Malelane and Pongola. CTV populations from these samples were analysed through the amplification of the p33 gene and subsequent direct Sanger sequencing. A subset of twelve of these amplicons was selected for deep Illumina MiSeq sequencing. The resulting data showed high intra-and inter-orchard diversity of CTV, with populations showing similar genotype compositions to those found in recent studies on the "Star Ruby" cultivar. Populations were 2 composed of a clearly dominant component, in most cases RB or Kpg3/SP/T3-like sequences, with at least two minor sequence types. AT-1-like and VT-like sequences were found to be dominant in only four and one of the analysed populations respectively. HA16-5, Taiwan-Pum/M/T5, T30 and B165 were present as intermittent minor components (less than 10% of mapped reads) across the populations.

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Section: Results Of Direct Sanger Sequences Of 35 Samples Are Listed Insupporting

confidence: 89%

Survey of Citrus tristeza virus (CTV) on Citrus paradisi (Macfad.) cv. “Marsh” in South Africa

Read

Palacios²,

Figueroa³

et al. 2018

Eur J Plant Pathol

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“…In the study of CTV diversity on pre-immunized Star Ruby plants in South Africa ( When compared with a previous study of CTV diversity on Citrus x limon (Lemon) cultivars in Tucumán province (Read et al 2017), strain profiles of the pigmented grapefruit cultivars from northwestern Argentina appear to be more diverse as well as more erratic in terms of the relative abundances of reads mapping to their references. The populations associated with lemon cultivars showed a much greater homogeneity with a relatively stable abundance of reads mapping to RB, Kpg3/SP/T3 and HA16-5 reference sequences.…”

Section: Discussionmentioning

confidence: 91%

“…In general, CTV diversity is poorly studied in South America (Benítez-Galeano et al 2015;Read et al 2017). The study by Iglesias et al (2008) has until now been the most recent, regarding genetic diversity of CTV within Argentinean grapefruit.…”

Section: Discussionmentioning

confidence: 99%

“…Other, minor components within the CTV populations associated with lemon were below or at the 20 limit of detection. Interestingly, greenhouse analyses of CTV populations associated with lemon cultivars from Tucumán province showed that they generally induced only mild stempitting symptoms on a limited number of experimental Duncan grapefruit plants (Read et al 2017). Therefore, CTV populations associated with lemon cultivars are probably not responsible for the stem-pitting and decline of grapefruit cultivars in northwestern Argentina.…”

Section: Discussionmentioning

confidence: 99%

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Survey of Citrus tristeza virus (CTV) diversity in pigmented Citrus x paradisi (Macfad.) (Grapefruit) trees in north-western Argentina

Read

Palacios²,

Kleynhans

et al. 2017

Eur J Plant Pathol

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Citrus tristeza virus (CTV) is the most severe viral pathogen of citrus and is responsible for eliciting a wide range of devastating disease symptoms. Grapefruit cultivars (Citrus x paradisi) are the most sensitive among citrus to the effects of CTV infections. Grapefruit is an important crop within the north-western Argentine citrus industry; however, production has been affected by CTV stem-pitting. In general, CTV diversity within South America is poorly studied, with data on grapefruit CTV populations being particularly limited. In this study, 50 samples were collected from Star Ruby, Henninger's Ruby and Ruben Pink cultivars, within the provinces of Tucumán, Salta and Jujuy in north-western Argentina. The CTV p33 gene was PCR amplified and the resulting amplicons sequenced with Sanger sequencing. A subset of these amplicons was sequenced with Illumina MiSeq sequencing.AT-1-like sequences were dominant within the majority of populations, as determined by Sanger sequencing, followed by sequences clustering within the unresolved Kpg3/SP/T3 and RB clades. Sequencing by Illumina MiSeq confirmed this, as well as detecting minor sequence types within the HA 16-5, VT, B165 and A18 clades.

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Citrus spp. (Citrus aurantifolia; C. aurantium; C. clementina; C. decumana; C. grandis; C. jambhiri; C. limon; C. medica; C. paradisi; C. reticulata; C. sinensis; C. unshia; C. volkameriana)

Sastry¹,

Mandal²,

Scott³

et al. 2019

Encyclopedia of Plant Viruses and Viroids

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Survey of citrus tristeza virus (CTV) strains in Citrus x limon (L) Burm f. (lemon) in Tucumán Province, Argentina

Cited by 4 publications

References 18 publications

Survey of Citrus tristeza virus (CTV) on Citrus paradisi (Macfad.) cv. “Marsh” in South Africa

Survey of Citrus tristeza virus (CTV) on Citrus paradisi (Macfad.) cv. “Marsh” in South Africa

Survey of Citrus tristeza virus (CTV) diversity in pigmented Citrus x paradisi (Macfad.) (Grapefruit) trees in north-western Argentina

Citrus spp. (Citrus aurantifolia; C. aurantium; C. clementina; C. decumana; C. grandis; C. jambhiri; C. limon; C. medica; C. paradisi; C. reticulata; C. sinensis; C. unshia; C. volkameriana)

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