Survey of Crimean-Congo Hemorrhagic Fever Enzootic Focus, Spain, 2011–2015

Negredo, Anabel; Habela, M.; Arellano, Eva Ramírez de; Díez, F. Najarro; Lasala, Fátima; López, Pablo; Sarria, Ana; Labiod, Nuria; Calero‐Bernal, Rafael; Arenas, Miguel; Tenorio, Antonio; Estrada-Peña, Agustı́n; Sánchez-Seco, María Paz

doi:10.3201/eid2506.180877

Cited by 47 publications

(42 citation statements)

References 36 publications

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“…In 2016, the first autochthonous human infection was recognised in a man who travelled to the province of Ávila, 300 km south-west of Caceres where the infected ticks had been identified [25]. After an extensive effort of public health vigilance, the virus was identified in ticks feeding on domestic and wild animals in western Spain [26][27][28]. This could be attributed to the silent circulation of CCHFV introduced some time ago from West Africa by migratory birds [26,29].…”

Section: Introductionmentioning

confidence: 99%

Crimean-Congo haemorrhagic fever (CCHF) virus-specific antibody detection in blood donors, Castile-León, Spain, summer 2017 and 2018

et al. 2020

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Background: Crimean-Congo haemorrhagic fever virus (CCHFV) is considered an emerging or even a probable re-emerging pathogen in southern Europe. Presence of this virus had been reported previously in Spain in 2010. Aim: We aimed to evaluate the potential circulation of CCHFV in western Spain with a serosurvey in asymptomatic adults (blood donors). Methods: During 2017 and 2018, we conducted a CCHFV serosurvey in randomly selected asymptomatic blood donors from western Spain. Three assays using specific IgG antibodies against CCHFV were performed: the VectoCrimea ELISA test, an in-house ELISA and indirect immunofluorescence (EuroImmun) test with glycoprotein and nucleoprotein. Results: A total of 516 blood donors participated in this cross-sectional study. The majority of the study participants were male (68.4%), and the mean age was 46.3 years. Most of the participants came from rural areas (86.8%) and 68.6% had contact with animals and 20.9% had animal husbandry practices. One in five participants (109/516, 21.1%) were engaged in at-risk professional activities such as agriculture and shepherding, slaughtering, hunting, veterinary and healthcare work (mainly nursing staff and laboratory technicians). A total of 15.3% of the participants were bitten by ticks in the days or months before the date of sampling. We detected anti-CCHFV IgG antibodies with two diagnostic assays in three of the 516 individuals and with one diagnostic assay in six of the 516 individuals. Conclusion: Seroprevalence of CCHFV was between 0.58% and 1.16% in Castile-León, Spain. This is the first study in western Spain that showed circulation of CCHFV in healthy people.

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Section: Introductionmentioning

confidence: 99%

Crimean-Congo haemorrhagic fever (CCHF) virus-specific antibody detection in blood donors, Castile-León, Spain, summer 2017 and 2018

et al. 2020

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“…More recently, some human cases have been reported for the first time in South-Eastern Europe (Mertens, Schmidt, Ozkul, & Groschup, 2013) and Spain (Negredo et al, 2019). The transmission to humans occurs through tick bites or exposure to blood or other body fluids of an infected animal or human (Bente et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Development of a multiplex assay for antibody detection in serum against pathogens affecting ruminants

Hoste

Ruiz²,

Fernández‐Pacheco

et al. 2020

Transbound Emerg Dis

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“…Moreover, pairwise distances between sequences were calculated using the Kimura model. In agreement with previous works, branch positions, pairwise distances, and collection countries were used to identify separate CCHFV clades within the phylogenetic tree [5,9,10,11,12,13,14,15,16,17,18,19,20,21,22].…”

Section: Methodsmentioning

confidence: 68%

“…Most of the isolates causing outbreaks in Eastern Europe belong to Clade V, whereas Clade VI includes largely divergent strains isolated from ticks in Greece (including the strain AP92) [4,18] and Turkey [19]. Isolates belonging to the “African” Clade III were also collected in Spain from infected ticks in 2010 [20] and during 2011–2015 [21], which were found to have caused human infections in 2016 [22]. One more recent human infection in Spain in 2018 were caused by a CCHFV not yet characterized [23].…”

Section: Introductionmentioning

confidence: 99%

Geographical Variability Affects CCHFV Detection by RT–PCR: A Tool for In-Silico Evaluation of Molecular Assays

Gruber¹,

Bartolini²,

Castilletti³

et al. 2019

Viruses

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The Crimean–Congo hemorrhagic fever virus (CCHFV) is considered to be a major emerging infectious threat, according to the WHO R&D blueprint. A wide range of CCHFV molecular assays have been developed, employing varied primer/probe combinations. The high genetic variability of CCHFV often hampers the efficacy of available molecular tests and can affect their diagnostic potential. Recently, increasing numbers of complete CCHFV genomic sequences have become available, allowing a better appreciation of the genomic evolution of this virus. We summarized the current knowledge on molecular methods and developed a new bioinformatics tool to evaluate the existing assays for CCHFV detection, with a special focus on strains circulating in different geographical areas. Twenty-two molecular methods and 181 sequences of CCHFV were collected, respectively, from PubMed and GenBank databases. Up to 28 mismatches between primers and probes of each assay and CCHFV strains were detected through in-silico PCR analysis. Combinations of up to three molecular methods markedly decreased the number of mismatches within most geographic areas. These results supported the good practice of CCHFV detection of performing more than one assay, aimed for different sequence targets. The choice of the most appropriate tests must take into account patient’s travel history and geographic distribution of the different CCHFV strains.

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Survey of Crimean-Congo Hemorrhagic Fever Enzootic Focus, Spain, 2011–2015

Cited by 47 publications

References 36 publications

Crimean-Congo haemorrhagic fever (CCHF) virus-specific antibody detection in blood donors, Castile-León, Spain, summer 2017 and 2018

Crimean-Congo haemorrhagic fever (CCHF) virus-specific antibody detection in blood donors, Castile-León, Spain, summer 2017 and 2018

Development of a multiplex assay for antibody detection in serum against pathogens affecting ruminants

Geographical Variability Affects CCHFV Detection by RT–PCR: A Tool for In-Silico Evaluation of Molecular Assays

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