Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit

Li, Ming; Sun, Min; Xia, Hong; Duan, Jinsheng; Du, Daolin

doi:10.3390/toxins11010006

Cited by 13 publications

(9 citation statements)

References 38 publications

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“…Compared with the commercial kits, the sensitivity and specificity is higher (3 ng/mL in the working buffer), the DON levels of samples were equivalent to a 100-fold dilution with an LOD of 300 ng/g in authentic agricultural samples, and the cross-reaction rate with 3-Ac-DON and 15-Ac-DON was less than 70%, less than 1%, respectively. Compared with the kit that was developed by Li et al [12], it has higher sensitivity and specificity (4.9 ng/mL in the working buffer), the DON levels of samples were equivalent to a 40-fold dilution with an LOD of 200 ng/g in authentic agricultural samples, and the cross-reaction rate with 3-Ac-DON and 15-Ac-DON was 5.7%, less than 0.5%, respectively. Accuracy and precision are measured by spiked sample recovery (%) and relative standard deviation (RSD%).…”

Section: Determination Of Dcelisa Kit Performancementioning

confidence: 94%

“…In view of its serious toxic effects, in the preliminary draft of the DON maximum levels (MLs), the Codex Alimentarius Food (FAO) Committee recommended the following limits: 2 mg/kg in unprocessed cereals, 1 mg/kg in semi-processed products using wheat, corn and barley as raw materials, and 0.5 mg/kg in cereals for infants and young children [10,11]. In China, the ML of DON in maize, wheat, and their products is regulated at 1 mg/kg [12,13]. The molecular formula of DON is C15H20O6, and its molecular weight is 296.32 [14].…”

Section: Introductionmentioning

confidence: 99%

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Development of a Direct Competitive ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat

et al. 2019

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This study was conducted to develop a self-assembled direct competitive enzyme-linked immunosorbent assay (dcELISA) kit for the detection of deoxynivalenol (DON) in food and feed grains. Based on the preparation of anti-DON monoclonal antibodies, we established a standard curve with dcELISA and optimized the detection conditions. The performance of the kit was evaluated by comparison with high-performance liquid chromatography (HPLC). The minimum detection limit of DON with the kit was 0.62 ng/mL, the linear range was from 1.0 to 113.24 ng/mL and the half-maximal inhibition concentration (IC 50 ) was 6.61 ng/mL in the working buffer; there was a limit of detection (LOD) of 62 ng/g, and the detection range was from 100 to 11324 ng/g in authentic agricultural samples. We examined four samples of wheat bran, wheat flour, corn flour and corn for DON recovery. The average recovery was in the range of 77.1% to 107.0%, and the relative standard deviation (RSD) ranged from 4.2% to 11.9%. In addition, the kit has the advantages of high specificity, good stability, a long effective life and negligible sample matrix interference. Finally, wheat samples from farms in the six provinces of Henan, Anhui, Hebei, Shandong, Jiangsu and Gansu in China were analyzed by the kit. A total of 30 samples were randomly checked (five samples in each province), and the results were in good agreement with the standardized HPLC method. These tests showed that the dcELISA kit had good performance and met relevant technical requirements, and it had the characteristics of accuracy, reliability, convenience and high-throughput screening for DON detection. Therefore, the developed kit is suitable for rapid screening of DON in marketed products.

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Section: Determination Of Dcelisa Kit Performancementioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Development of a Direct Competitive ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat

et al. 2019

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“…Studies to determine the mycotoxin content of food products produced in different countries and sold in markets have accelerated all over the world (Reddy et al, 2009;Houissa et al, 2019;Potortì et al, 2020;Costa et al, 2019). Li, Sun, Hong, Duan, and Du (2019) detected 88.7% contamination in their DON study on 328 agricultural products with ELISA, ranging from 0.2009 to 6.4806 μg kg −1 . In another study, El-Shanshoury, El-Sabbagh, Emara, and Saba (2014) investigated the AFT and AFB 1 values of cereal grains and peanut sold in central delta provinces markets, Egypt by using thin layer chromatography method.…”

Section: Discussionmentioning

confidence: 99%

Incidence of aflatoxins, ochratoxin A, zearalenone, and deoxynivalenol in food commodities from Turkey

Cüce

2020

Journal of Food Safety

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Aspergillus, Penicillium, Fusarium, and Alternaria species which produce toxic metabolites, create a big problem in terms of the production of reliable human food and animal feeds. This study was conducted to determine the mycotoxin contents of foodstuff collected uniformly in each year of 2017, 2018, and 2019 in Şebinkarahisar, Turkey by enzyme-linked immunosorbent assay. The highest total aflatoxin, and aflatoxin B1 were determined in red pepper in 2018 with 6.47 ± 0.07 and 4.58 ± 0.01 μg kg −1 , respectively while the highest ochratoxin amount was obtained again in the red paper in 2019 with 7.97 ± 0.57 μg kg −1. Contrarily, the highest incidence of zearalenone and deoxynivalenol was determined in wheat and corn flour with 12.49 ± 0.3 and 397.6 ± 7.34 μg kg −1 , respectively. Weather changes have also been found to affect the incidence of mycotoxin. But, determined mycotoxin values do not pose a risk according to the Turkish Food Codex Contaminants Regulation. 1 | INTRODUCTION Mycotoxins are generally known as aflatoxins (AFs) (aflatoxin B 1 [AFB 1 ], aflatoxin B2 [AFB 2 ], aflatoxin G1 [AFG 1 ], aflatoxin G2 [AFG 2 ]) ochratoxin A (OTA), zearalenone (ZEN), and deoxynivalenol (DON). These substances are important secondary metabolites produced by particularly various strains of Aspergillus, Penicillium, Fusarium, and

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“…and mainly contaminates wheat, maize and other crops in the field and during storage ( Mishra et al, 2019 ). DON seriously threatens the health of humans and animals, as it shows enterotoxicity, immunotoxicity and teratogenicity ( Li et al, 2018a ; Liao et al, 2018 ). Pigs are the most sensitive species to DON and intestine is the most important target organ of DON which could induce intestinal inflammation and disrupt the intestinal barrier function ( Alassane-Kpembi et al, 2017 ; Ananthakrishnan et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Nontoxic-dose deoxynivalenol aggravates lipopolysaccharides-induced inflammation and tight junction disorder in IPEC-J2 cells through activation of NF-κB and LC3B

Liu

Lin

et al. 2020

Food and Chemical Toxicology

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Lipopolysaccharide (LPS) is the key factor in various intestinal inflammation which could disrupt the epithelial barrier function. Deoxynivalenol (DON), a well-known mycotoxin, can induce intestinal injury. However, the combined enterotoxicity of LPS and DON has rarely been studied. In this study, IPEC-J2 cell monolayers were exposed to LPS and nontoxic-dose DON for 12 and 24 h to investigate the effects of DON on LPS-induced inflammatory response and tight junction variation, and specific inhibitor and CRISPR-Cas9 were used to explore the underlying mechanisms. Our results showed that nontoxic-dose DON aggravated LPS-induced cellular inflammatory response, reflecting on more significant changes of inflammatory cytokines mRNA expression, higher protein expression of NOD-like receptor protein 3 (NLRP3) and procaspase-1. Moreover, nontoxic-dose DON aggravated LPS-induced mRNA and protein expression decreased, and distribution confused of tight junction proteins. We found that DON further enhanced LPS-induced phosphorylation and nucleus translocation of p65, and expression of LC3B-Ⅱ. NF-κB inhibitor and CRISPR-Cas9-mediated knockout of LC3B attenuated the effects of combination which indicated nontoxic-dose DON aggravated LPS-induced intestinal inflammation and tight junction disorder through activating NF-κB signaling pathway and autophagy-related protein LC3B. It further warns that ingesting low doses of mycotoxins may exacerbate the effects of intestinal pathogens on the body.

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Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit

Cited by 13 publications

References 38 publications

Development of a Direct Competitive ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat

Development of a Direct Competitive ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat

Incidence of aflatoxins, ochratoxin A, zearalenone, and deoxynivalenol in food commodities from Turkey

Nontoxic-dose deoxynivalenol aggravates lipopolysaccharides-induced inflammation and tight junction disorder in IPEC-J2 cells through activation of NF-κB and LC3B

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