Survey of environmental biocontamination on board the International Space Station

Novikova, Natalia; Boever, Patrick De; Poddubko, S. V.; Deshevaya, Elena; Polikarpov, N.; Rakova, N.; Coninx, Ilse; Mergeay, Max

doi:10.1016/j.resmic.2005.07.010

Cited by 216 publications

(183 citation statements)

References 22 publications

Supporting

Mentioning

174

Contrasting

Unclassified

Order By: Relevance

“…Especially in prokaryotes, where the number of sequenced genomes will soon be counted in thousands, understanding how contemporary organisms evolved their range of functions is challenging and fascinating. Prokaryotes certainly provide an interesting opportunity for studying the mechanisms of evolution: they harbor a previously unsuspected diversity even within species and populations [1], they are found in small [2] to very large population sizes [3], they can survive or prosper in most inhospitable environments from the inside of eukaryotic cells [2] to hot springs [4] or spaceships [5], and they frequently acquire and use genetic material from distantly related organisms.…”

mentioning

confidence: 99%

Comparative genomics and the evolution of prokaryotes

Abby

Daubin

2007

Trends in Microbiology

View full text Add to dashboard Cite

mentioning

confidence: 99%

Comparative genomics and the evolution of prokaryotes

Abby

Daubin

2007

Trends in Microbiology

View full text Add to dashboard Cite

“…Подавляющее большинство исследований с жи-выми организмами проводилось на НОО, характе-ризующейся высоким уровнем радиации, вакуумом и экстремальными температурами [2,3,5,11]. Наше исследование также проходило на НОО.…”

Section: обсуждение результатовunclassified

“…Что касается астробиологических исследований, проводившихся с грибами, то, по данным литера-туры, микромицеты обнаружены внутри Между-народной космической станции (МКС) [11] и станции "Мир" [12]. Кроме того, чёрные дрожже-подобные грибы выжили как в искусственно соз-данных условиях открытого космоса и Марса в ла-боратории, так и после 1,5-летнего космического полёта при наличии защиты от полного спектра космического излучения [3].…”

unclassified

The survival of micromycetes exposed to space conditions

Ponizovskaya

Dyakov

Antropova

et al. 2017

Moscow Univ. Biol.Sci. Bull.

View full text Add to dashboard Cite

Изучение жизнеспособности микромицетов в условиях космического полёта проводили на российском научно-исследовательском спутнике Фотон-М4. Длительность полёта спутника составила 45 сут. Для исследования были отобраны 13 видов грибов из 10 ро-дов, выделенные из строительных материалов на минеральной основе. Споры грибов вносили в стерильный кварцевый песок; песок со спорами помещали в полипропиленовые пробирки фирмы Eppendorf (Германия). Во время космического полёта пробирки со спо-рами находились либо внутри спутника в контейнерах "Биоконт", либо снаружи спут-ника в контейнерах "Экзобиофрост". При взлёте и посадке контейнеры "Экзобиофрост" были плотно закрыты крышкой, а на орбите во время всего полёта крышка модуля с кон-тейнерами была открыта для помещения образцов в условия открытого космоса. Споры 10 из 13 (77%) исследованных видов грибов (Acremonium charticola, Aspergillus niger, Aspergillus versicolor, Chaetomium globosum, Cladosporium sphaerospermum, Penicillium chrysogenum, Penicillium verrucosum, Purpureocillium lilacinum, Sarocladium kiliense и Trichoderma harzianum) выжили после полёта как внутри, так и снаружи спутника. Споры лишь 3 видов (23% от всех исследуемых видов), Acremonium furcatum, Engyodontium album и Verticillium zaregamsianum, полностью погибли после полёта как внутри, так и снаружи спутника. Выживае-мость спор варьировала в зависимости от вида гриба. Таким образом, споры грибов обла-дали способностью выживать при действии комплекса стрессовых факторов, таких как низкая температура, радиация и т.д. Микромицеты можно рассматривать как модель вы-сокой устойчивости эукариотических организмов к стрессовым факторам, предел эколо-гической пластичности которых крайне высок и даже выходит за границы этого показа-теля для земных местообитаний.Ключевые слова: экзобиология, Фотон-М4, космический полёт, факторы космического полёта, грибы, микромицеты, жизнеспособность.

show abstract

“…However various experimental results in space have shown that thick layers of spores, having the appropriate nutrients, could survive as long as 6 years (Horneck et al, 2001;Horneck et al 2002) and certain types of fungi, not only can survive inside a space vehicle at low gravity conditions, but also indicate intense bioactivity causing biodegradation of spacecraft's materials (Kern and Hock, 1993;Zimmermann et al, 1994;Hahn and Hock 1999). For example, the fungal growth inside the MIR station in 1995 was from the descendants of fungi from 1988 and further investigations identified the presence of several microorganisms, which not only effected the optimal performance of the space crew, but also damaged the metals and the polymeric parts of the functional units, causing in some cases serious malfunction of the vehicle's systems (Novikova et al, 2006). It is evident therefore that additional data concerning long duration exposure of fungal spores under space conditions and at different wavelengths is needed in order to: 1) either sustain the hypothesis of interplanetary or interstellar transport of life or 2) find the optimum conditions for minimal growth and survival of fungi in space vehicles.…”

Section: Introductionmentioning

confidence: 99%

Interplanetary survival probability of Aspergillus terreus spores under simulated solar vacuum ultraviolet irradiation

Sarantopoulou¹,

Gomoiu²,

Kollia³

et al. 2011

Planetary and Space Science

View full text Add to dashboard Cite

This work is a part of ESA/EU SURE project aiming to quantify the survival probability of fungal spores in space under solar irradiation in the vacuum ultraviolet (VUV) (110-180 nm) spectral region. The contribution and impact of VUV photons, vacuum, low temperature and their synergies, on the survival probability of Aspergillus terreus spores is measured at simulated space conditions on Earth. To simulate the solar VUV irradiation, the spores are irradiated with a continuous discharge VUV hydrogen photon source and a molecular fluorine laser, at low and high photon intensities at 10 15 photon m -2 s -1 and 3.9 x 10 27 photons pulse -1 m -2 s -1 respectively. The survival probability of spores is independent from the intensity and the fluence of photons, within certain limits, in agreement with previous studies. The spores are shielded from a thin carbon layer, which is formed quickly on the external surface of the proteinaceous membrane at higher photon intensities at the start of the VUV irradiation. Extrapolated the results in space conditions, for an interplanetary direct transfer orbit from Mars to Earth, the spores will be irradiated with 3.3 x 10 21 solar VUV photons m -2 . This photon fluence is equivalent to the irradiation of spores on Earth with 54 laser pulses with an experimental ~92% survival probability, disregarding the contribution of space vacuum and low temperature, or to continuous solar VUV irradiation for 38 days in space near the Earth with an extrapolated ~61% survival probability. The experimental results indicate that the damage of spores is mainly from the dehydration stress in vacuum. The high survival probability after 4 days in vacuum (~34 %), is due to the exudation of proteins on the external membrane, preventing thus further dehydration of spores. In addition, the survival probability is increasing to (~54%) at 10 K with 0.12 K/s cooling and heating rate.

show abstract

Survey of environmental biocontamination on board the International Space Station

Cited by 216 publications

References 22 publications

Comparative genomics and the evolution of prokaryotes

Comparative genomics and the evolution of prokaryotes

The survival of micromycetes exposed to space conditions

Interplanetary survival probability of Aspergillus terreus spores under simulated solar vacuum ultraviolet irradiation

Contact Info

Product

Resources

About