Survey Of Infectious Agents Associated With Porcine Respiratory Disease Complex (PRDC) In Serbian Swine Herds Using Polymerase Chain Reaction (PCR) Detection

Božidar, Savić; Oliver, Radanović; Jovičić, Dubravka; Nešić, Ksenija; Snežana, Ivanović; Stevančević, Ognjen; Cvetojević, Đorđe; Dragan, Kasagić

doi:10.1515/acve-2015-0006

Cited by 9 publications

(2 citation statements)

References 22 publications

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“…However, comparability of IAV detection rates amongst different specimens is limited, as IAV RNA was only detected in a total of 29 samples. Low positivity rates for IAV RNA (6.1%) from all samples that were tested for the pathogen (n = 479) were already demonstrated before [34,54]. This could be linked to various reasons.…”

Section: Discussionmentioning

confidence: 96%

Retrospective Analysis of the Detection of Pathogens Associated with the Porcine Respiratory Disease Complex in Routine Diagnostic Samples from Austrian Swine Stocks

Renzhammer,

Auer,

Loncaric

et al. 2023

Veterinary Sciences

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The diagnostic workup of respiratory disease in pigs is complex due to coinfections and non-infectious causes. The detection of pathogens associated with respiratory disease is a pivotal part of the diagnostic workup for respiratory disease. We aimed to report how frequently certain viruses and bacteria were detected in samples from pigs with respiratory symptoms in the course of routine diagnostic procedures. Altogether, 1975 routine diagnostic samples from pigs in Austrian swine stocks between 2016 and 2021 were analysed. PCR was performed to detect various pathogens, including porcine reproductive and respiratory syndrome virus (PRRSV) (n = 921), influenza A virus (n = 479), porcine circovirus type 2 (PCV2) (n = 518), Mycoplasma (M.) hyopneumoniae (n = 713), Actinobacillus pleuropneumoniae (n = 198), Glaesserella (G.) parasuis (n = 165) and M. hyorhinis (n = 180). M. hyorhinis (55.1%) had the highest detection rate, followed by PCV2 (38.0%) and Streptococcus (S.) suis (30.6%). PRRSV was detected most frequently in a pool of lung, tonsil and tracheobronchial lymph node (36.2%). G. parasuis was isolated more frequently from samples taken after euthanasia compared to field samples. PRRSV-positive samples were more likely to be positive for PCV2 (p = 0.001), M. hyopneumoniae (p = 0.032) and Pasteurella multocida (p < 0.001). M. hyopneumoniae-positive samples were more likely to be positive for P. multocida (p < 0.001) and S. suis (p = 0.046), but less likely for M. hyorhinis (p = 0.004). In conclusion, our data provide evidence that lung samples that were positive for a primary pathogenic agent were more likely to be positive for a secondary pathogenic agent.

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Section: Discussionmentioning

confidence: 96%

Retrospective Analysis of the Detection of Pathogens Associated with the Porcine Respiratory Disease Complex in Routine Diagnostic Samples from Austrian Swine Stocks

Renzhammer,

Auer,

Loncaric

et al. 2023

Veterinary Sciences

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Pasteurella multocida (P. multocida) is a highly infectious pathogen capable of co-infecting poorly maintained livestock with other pathogens like Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, Bordetella bronchiseptica, porcine reproductive and respiratory syndrome virus, and porcine Circovirus, resulting in severe respiratory diseases or atrophic rhinitis in the swine industry (4,6,15,17,21,22). The serotype distributions and phenotypic characteristics of P. multocida show both temporal and geographical variations.

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confidence: 99%

Capsular types, LPS genotypes, and virulence-associated genes of Pasteurella multocida strains isolated from pigs

Li¹,

Fang²,

Luo³

et al. 2020

Medycyna Weterynaryjna

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Pasteurella multocida (P. multocida), an opportunistic zoonotic pathogen associated with high morbidity and mortality in livestock, shows significant temporal and geographical variation in its serotype distributions and phenotypic characteristics. The aim of this study was To investigate capsular types, lipopolysaccharide (LPS) genotypes, and virulence-associated genes of P. multocida strains isolated from pigs. A total of 801 samples (lungs, tonsils, nasal swabs) were collected from slaughterhouses and various regions of the Hunan province. P. multocida strains were isolated from various samples, classified, and virulence-associated genes were detected by polymerase chain reaction (PCR). 124 P. multocida strains were assigned to six groups based on both capsular type and LPS genotype, namely A: L3 (capsular type A and LPS genotype 3, 64/124); A: L6 (16/124); D: L6 (38/124); F: L3 (4/124); L3 (1/124) and 1 untypable strain. Of the 23 virulence-associated genes investigated in this study, 14 were highly expressed in 98% to 100% of the 124 strains. While tbpA was undetectable in any of the isolated strains, hsf-1, pfhA, tadD, toxA, pmHAS, hgbA, hgbB, and nanB showed differential distribution among the strain groups. Interestingly, pfhA (Mutation or inactivation of pfhA was reported to decrease the virulence of P. multocida) was found in 46% of group A: L3 strains and in 100% of group F: L3 strains, but not found in other groups. Further investigation is needed to determine whether strains in group A: L3show greater virulence than the A: L6 P. multocida strains.

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Pig Diseases in Papua Province, Indonesia: Aetiology, Eco-epidemiology and Control Options

Nugroho

Kirkwood

Reichel

2016

Springer Science Reviews

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Survey Of Infectious Agents Associated With Porcine Respiratory Disease Complex (PRDC) In Serbian Swine Herds Using Polymerase Chain Reaction (PCR) Detection

Cited by 9 publications

References 22 publications

Retrospective Analysis of the Detection of Pathogens Associated with the Porcine Respiratory Disease Complex in Routine Diagnostic Samples from Austrian Swine Stocks

Retrospective Analysis of the Detection of Pathogens Associated with the Porcine Respiratory Disease Complex in Routine Diagnostic Samples from Austrian Swine Stocks

Capsular types, LPS genotypes, and virulence-associated genes of Pasteurella multocida strains isolated from pigs

Pig Diseases in Papua Province, Indonesia: Aetiology, Eco-epidemiology and Control Options

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