Survey of Methicillin-Resistant
            <i>Staphylococcus aureus</i>
            Strains from Two Hospitals in El Paso, Texas

O’Brien, Frances; Lim, Tien Tze; Winnett, David C.; Coombs, Geoffrey W.; Pearson, Julie; Delgado, A.; Langevin, Mark J.; Cantore, Stephanie A.; Gonzalez, Leti; Gustafson, John E.

doi:10.1128/jcm.43.6.2969-2972.2005

Cited by 15 publications

(9 citation statements)

References 21 publications

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“…It is of public health and scientific importance, therefore, to determine if MRSA/SCC mec in the border region is evolving differently than elsewhere in the United States, and whether the uniquely transitional environment of the border region may promote the emergence of new, more serious strains of MRSA that have the potential to spread throughout the rest of the United States. To date, limited studies have been undertaken to study MRSA/SCC mec along the U.S./Mexico border, and those that have, have focused on strains obtained from a hospital setting [5, 14, 17]. Thus very little is currently known about MRSA, and their SCC mec elements, in communities located along the Texas border with Mexico.…”

Section: Introductionmentioning

confidence: 99%

An Exploratory Study of Methicillin-Resistant Staphylococcus aureus and SCCmec Elements Obtained from a Community Setting Along the Texas Border with Mexico

Ammons¹,

Puttagunta²,

Granados³

et al. 2009

Curr Microbiol

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An exploratory study of methicillin-resistant Staphylococcus aureus (MRSA) and SCCmec elements in bacteria along the Mexican border of south Texas was performed. Between September and December of 2008, 375 swabs of anterior nares were self-collected by students attending the University of Texas-Pan American (UTPA) and cultured for MRSA. Fifty seven bacterial isolates were kept for further analysis that included suspected MRSA and other SCCmec-containing bacteria. Isolates were examined for the presence of nuc, mecA, lukS-PV, and spa genes using PCR. SCCmec and spa typing were also performed. Seven S. aureus isolates were found of which six were classified as MRSA. SCCmec typing showed five of the six MRSA strains to be type IV, while one MRSA strain, and most of the non-S. aureus strains, were untypeable, producing results that were indicative of mixed SCCmec types. Five of the six MRSA strains contained known spa types (two of which corresponded to USA300 and one to USA600), while one strain had a novel spa type. Only one isolate, a USA300 MRSA, was positive for lukS-PV. Easy access by the Texas border community to antibiotics in Mexico without a prescription, and the strong partition in SCCmec types between MRSA and non-S. aureus bacteria suggest that this border region of Texas may be uniquely suited for the study of emerging SCCmec types, their horizontal transfer, and perhaps other aspects of antibiotic resistance in bacteria.

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Section: Introductionmentioning

confidence: 99%

An Exploratory Study of Methicillin-Resistant Staphylococcus aureus and SCCmec Elements Obtained from a Community Setting Along the Texas Border with Mexico

Ammons¹,

Puttagunta²,

Granados³

et al. 2009

Curr Microbiol

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“…Since 2000, the New York/Japan clone has been reported in several US states, [7][8][9] Canada, 10 Brazil, 11 Mexico, 12 China, 13 and Korea. 14,15 Although infrequently reported in Europe, the New York/Japan clone has recently been described as a major epidemic MRSA (EMRSA) clone in Hungary.…”

mentioning

confidence: 99%

Controlling a Multicenter Outbreak Involving the New York/Japan Methicillin-ResistantStaphylococcus aureusClone

Coombs

Gessel

Pearson

et al. 2007

Infect. Control Hosp. Epidemiol.

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objective. To describe the control of an outbreak of infection and colonization with the New York/Japan methicillin-resistant Staphylococcus aureus (MRSA) clone in multiple healthcare facilities, and to demonstrate the importance of making an MRSA management policy involving molecular typing of MRSA into a statewide public health responsibility.setting. A range of healthcare facilities, including 2 metropolitan teaching hospitals and a regional hospital, as well as several community hospitals and long-term care facilities in a nonmetropolitan healthcare region.interventions. A comprehensive, statewide MRSA epidemiological investigation and management policy.

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“…The MLST website revealed that strain H29 is ST5 (1,4,1,4,12,1,10), an ST that contains human MRSA isolates and is found within CC5. The presence of ST5 MRSA strains circulating in Paso del Norte area hospitals located near the dairy investigated has previously been reported (O’Brien et al, 2005; Delgado et al, 2007). …”

Section: Resultsmentioning

confidence: 82%

“…The plugs were electrophoresed in a 1% (wt/vol) agarose gel for 23 h at 6 V/cm, with an initial switch time of 1 s and final switch time of 30 s at 11.3°C using the CHEF DR III system (Bio-Rad Laboratories Inc., Hercules, CA). Band patterns were compared using the Fingerprinting II Informatix software (Bio-Rad Laboratories Inc.) to determine the clonal relatedness (O’Brien et al, 2005). Strains with 80% or greater pattern similarity were considered clonal (Tenover et al, 1995).…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of Staphylococcus aureus strains from a Paso del Norte dairy

Matyi

Dupre

Johnson

et al. 2013

Journal of Dairy Science

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The primary purpose of this study was to determine if methicillin-resistant Staphylococcus aureus (MRSA) strains could be identified in the milk of dairy cattle in a Paso del Norte region dairy of the United States. Using physiological and PCR-based identification schemes, a total of 40 Staph. aureus strains were isolated from 29 raw milk samples of 133 total samples analyzed. Pulsed-field gel electrophoresis after digestion with the SmaI enzyme revealed that the 40 confirmed strains were represented by 5 pulsed-field types, which each contained 3 or more strains. Of 7 hospital strains isolated from cows undergoing antibiotic therapy, 3 demonstrated resistance to 3 or more antimicrobial classes and displayed similar pulsed-field gel electrophoresis patterns. A secondary purpose of this study was to elucidate the evolutionary relationships of strains isolated in this study to genomically characterized Staph. aureus strains. Therefore, Roche 454 GS (Roche Diagnostics Corp., Dallas, TX) pyrosequencing was used to produce draft genome sequences of an MRSA raw milk isolate (H29) and a methicillin-susceptible Staph. aureus (PB32). Analysis using the BLASTn database (http://blast.ncbi.nlm.nih.gov/) demonstrated that the H29 draft genome was highly homologous to the human MRSA strain JH1, yet the β-lactamase plasmid carried by H29 was different from that carried by JH1. Genomic analysis of H29 also clearly explained the multidrug resistance phenotype of this raw milk isolate. Analysis of the PB32 draft genome (using BLASTn) demonstrated that this raw milk isolate was most related to human MRSA strain 04-02981. Although PB32 is not a MRSA, the PB32 draft genome did reveal the presence of a unique staphylococcal cassette mec (SCCmec) remnant. In addition, the PB32 draft genome revealed the presence of a novel bovine staphylococcal pathogenicity island, SaPIbovPB32. This study demonstrates the presence of clones closely related to human and (or) bovine Staph. aureus strains circulating in a dairy herd.

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Survey of Methicillin-Resistant Staphylococcus aureus Strains from Two Hospitals in El Paso, Texas

Cited by 15 publications

References 21 publications

An Exploratory Study of Methicillin-Resistant Staphylococcus aureus and SCCmec Elements Obtained from a Community Setting Along the Texas Border with Mexico

An Exploratory Study of Methicillin-Resistant Staphylococcus aureus and SCCmec Elements Obtained from a Community Setting Along the Texas Border with Mexico

Controlling a Multicenter Outbreak Involving the New York/Japan Methicillin-ResistantStaphylococcus aureusClone

Isolation and characterization of Staphylococcus aureus strains from a Paso del Norte dairy

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