Survey of multiply resistant Providencia stuartii in a chronic care unit

Swiatlo, Edwin; Kocka, Frank E.; Chittom, A L; Kantor, Harvey S.; Gac, Sylvie Le; Waiters, Lorna

doi:10.1016/0195-6701(87)90058-2

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…Strains of P. stuartii are commonly resistant to many drugs (9,14,27,28). The finding in the present study that 32.6% of the strains were resistant to ciprofloxacin, which has not been reported previously, is probably due to the frequent use of this antibiotic in nursing homes.…”

Section: Discussioncontrasting

confidence: 44%

Molecular epidemiology of catheter-associated bacteriuria in nursing home patients

et al. 1994

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Urine samples from 19 nursing home patients with long-term urinary catheters were cultured every 3 months for 18 months. Providencia stuartii, present in 74% of the elderly and in 59% of urine specimens, was the most frequently isolated bacteria. The persistence of P. stuartii was significantly higher among females than among males. In order to study the epidemiology of bacteriuria in this nursing home, bacteria were characterized by biochemical tests, antibiotic susceptibility patterns, and restriction fragment length polymorphism (RFLP) analysis. The antibiotic susceptibility pattern indicated that each patient had two to three different strains of P. stuartii during the 18 months of follow-up. In contrast, the RFLP analysis revealed that a specific strain had persisted in the urinary tract of the patient during the entire follow-up period. According to the biochemical profile, 74% of the patients had the same bacteria in urine cultures, pointing to a common source of transmission. RFLP analysis, however, demonstrated different patterns of RFLP, suggesting concomitant multiple sources of infection.

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Section: Discussioncontrasting

confidence: 44%

Molecular epidemiology of catheter-associated bacteriuria in nursing home patients

et al. 1994

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Preventing infection in healthcare settings

Gould¹,

Brooker²

2008

Infection Prevention and Control

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Numerical analysis of electrophoretic protein patterns of Providencia stuartii strains from urine, wound and other clinical sources

Costas¹,

Holmes²,

Wood³

1990

Journal of Applied Bacteriology

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Eighty-six strains of Providencia stuartii (mainly of human origin) were characterized by one-dimensional SDS-PAGE of cellular proteins. The strains came from various countries; 52 were from urine, 11 from wounds, five from blood (one of these also from urine), four from ear infections, two each from faeces and sputum, one from 'alimentation' and nine from unknown sources. The protein patterns, which contained 45 to 50 discrete bands, were highly reproducible. The patterns of 46 Prov. stuartii strains (selected to represent the full range of protein pattern diversity) plus those of the type strains of the four other Providencia species were used as the basis for two numerical analyses. In the first, which included all the protein bands, the Prov. stuartii strains formed 13 clusters at the 88% S level. In the second analysis, in which the principal protein bands (in the 33.8-40.7 kDa range) were excluded, 45 of the 46 Prov. stuartii strains formed a single cluster at the 82% S level, whilst the four Providencia reference strains remained unclustered. The 40 strains of Prov. stuartii not included in the cluster analysis were assigned to a protein type by calculating their similarity with the strains in the database used for the cluster analysis. We conclude that high resolution PAGE combined with computerized analysis of protein patterns provides the basis for typing clinical strains of Prov. stuartii. Reference strains of each of the 13 PAGE types identified are available from NCTC for inclusion in future studies.

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Survey of multiply resistant Providencia stuartii in a chronic care unit

Cited by 4 publications

References 9 publications

Molecular epidemiology of catheter-associated bacteriuria in nursing home patients

Molecular epidemiology of catheter-associated bacteriuria in nursing home patients

Preventing infection in healthcare settings

Numerical analysis of electrophoretic protein patterns of Providencia stuartii strains from urine, wound and other clinical sources

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