Survey of Pathogens Isolated from Mussels Perna Perna Collected in Rocky Shore and Fishmarket of Niterói, RJ, and Their Respective Resistance Profile to Antimicrobial Drugs

Abstract

To assess bacteriological safety of mussels Perna perna, harvested in Itaipu, Niter oi, RJ and purchased at São Pedro fishmarket at the same city, fecal coliform counts and detection of Escherichia coli, Salmonella spp. and Vibrio spp. was performed in 27 mussel and 9 seawater samples collected between March 2012 and April of 2013. The pathogens isolated were submitted to antimicrobial susceptibility test.The majority (74%) of the mussel samples were unfit for human consumption and 89% of the seawater samples had unsatisfactorily high levels of contamination according to Brazilian laws. From the 77 E. coli and 51 Vibrio spp. isolated, 13% and 68.6% showed multiresistance and 15.6% and 72.5% showed multidrug resistant, respectively. The only Salmonella strain isolated was susceptible to all antimicrobial tested. Serotypes of Salmonella spp. and V. cholerae were not detected from the enrichment broths. Water pollution and food manipulation, throughout the food production chain, were factors which influenced the contamination of mussels. PRACTICAL APPLICATIONSThis is the first report studying microbial quality of mussels along their process before arriving at the market; including characteristics of virulence genes and antimicrobial resistance of pathogens. Our study demonstrated that after collection, the manipulation realized by mussel's pickers is an important source of contamination, warning to the necessity of a better monitoring in the commercialization of this seafood.

Occurrence and distribution of fecal indicators and pathogenic bacteria in seawater and Perna perna mussel in the Gulf of Annaba (Southern Mediterranean)

“…Data obtained showed that the levels of FIB were alarmingly higher in P. perna than in the surrounding seawaters during all the study period. These outcomes are consistent with those reported in other coastal regions worldwide, suggesting that this strong accumulation capacity is mainly related to the filter-feeding behavior of these sentinel organisms, which make them one of the best bio-indicators of fecal pollution in coastal waters (Stabili et al 2005;Martinez and Oliveira 2010;Jayme et al 2016;Bozcal and Dagdeviren 2020). Furthermore, the levels of intestinal indicators in all sampling sites were well above the permissible limits recommended according to Regulation (854/2004/EC) of 29 April 2014 for human consumption, which recommends less than 230 E. coli/100g.…”

Assessing the Microbial Quality of Shrimp (Xiphonaeus kroyeri) and Mussels (Perna perna) Illegally Sold in the Vitória Region, Brazil, and Investigating the Antimicrobial Resistance of Escherichia coli Isolates

“…However, β-lactam antibiotics are commonly reported in aquatic environments mainly due to wastewater, sewage, and farm discharges as common contamination sources [44]. This contributes to the detection of isolates of E. coli resistant to β-lactams, which have been widely reported in Brazil, such as in aquatic environments [45,46], sea fishes [47], bivalves [48][49][50], and shrimp [51], highlighting the potential risks to public health, as shown by our data.…”