Survey of strain distribution and antibiotic resistance pattern of group B streptococci (Streptococcus agalactiae) isolated from clinical specimens

Mousavi, Seyed Masoud; Nasaj, Mona; Hosseini, Seyed Mostafa; Arabestani, Mohammad Reza

doi:10.3205/dgkh000278

Cited by 13 publications

(3 citation statements)

References 24 publications

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“…In the present study, the rates of resistance to erythromycin and clindamycin were reported as 73.7% and 52.6%, respectively. However, the erythromycin resistance rate among GBS isolates in the present study was relatively higher when compared with other studies in Iran, 34 35 36 but the high rates of resistance to erythromycin in GBS were reported in China (92.5% and 84.6%), Iraq (58.6%) and the USA (50.7%). 37 38 39 40 Furthermore, high resistance rates of GBS strains to clindamycin were reported from Iran (92.2%) and other countries such as China (55.7% and 87.5%), Iraq (45.6%) and Italy (32.20%).…”

Section: Discussioncontrasting

confidence: 87%

Antibiotic Susceptibility Patterns and Prevalence of Streptococcus Agalactiae Rectovaginal Colonization Among Pregnant Women in Iran

Dashtizade

Zolfaghari

Yousefi

et al. 2020

Rev Bras Ginecol Obstet

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Objective Streptococcus agalactiae is an important pathogen in neonates and pregnant women. Neonatal invasive infections due to S. agalactiae are life-threatening and preventive strategies for this challenge of human have become a concern. The aim of the present study was to determine the prevalence of rectovaginal colonization, related risk factors and antibiotic resistance pattern of S. agalactiae among pregnant women in Iran. Methods The present study was performed on 240 pregnant women. Vaginal and rectal swabs were obtained from all of the women and then were transferred to the laboratory. The isolation and identification of S. agalactiae was performed by standard microbiological tests and polymerase chain reaction (PCR) assay. The antimicrobial susceptibility patterns of the isolates were determined by the Kirby-Bauer disk diffusion. Polymerase chain reaction was used to detect ermB and mefA genes in erythromycin-nonsusceptible isolates. Results Out of 240 pregnant women, 16 cases (6.7%) were colonized by S. agalactiae. There is no significant association between demographic-obstetric factors and maternal S. agalactiae colonization in the pregnant women. Linezolid, vancomycin and ampicillin were the most effective antibiotics against S. agalactiae. The ermB gene was present in 6 (35.29%) S. agalactiae isolates. However, the mefA gene was not detected in any of the isolates. Conclusion Given the relatively significant prevalence of S. agalactiae colonization in the pregnant women in the present study and the risk of serious neonatal infections, the screening of pregnant mothers for the bacteria seems necessary. Our findings highlight the importance of appropriate antibiotic prophylaxis during pregnancy for the prevention of early onset S. agalactiae-neonatal infection and comorbidity.

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Section: Discussioncontrasting

confidence: 87%

Antibiotic Susceptibility Patterns and Prevalence of Streptococcus Agalactiae Rectovaginal Colonization Among Pregnant Women in Iran

Dashtizade

Zolfaghari

Yousefi

et al. 2020

Rev Bras Ginecol Obstet

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“…Briefly, the inhibition zone sizes were recorded for each antimicrobial agent and interpreted according to the Clinical Laboratory Standards Institute (CLSI) 2022 guidelines [ 5 ]. For this method, erythromycin and clindamycin disks were adjacent and 1 cm apart to identify clindamycin resistance induction via the D-zone test, as previously described [ 5 , 40 ]. Briefly, blunting (or D appearance) or bacterial growth within the clindamycin zone of inhibition proximal to the erythromycin disk was documented as inducible resistance, commonly mediated by MLS B -inducible methylation.…”

Section: Methodsmentioning

confidence: 99%

Genomic Analysis Reveals New Integrative Conjugal Elements and Transposons in GBS Conferring Antimicrobial Resistance

et al. 2023

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Streptococcus agalactiae or group B streptococcus (GBS) is a leading cause of neonatal sepsis and increasingly found as an invasive pathogen in older patient populations. Beta-lactam antibiotics remain the most effective therapeutic with resistance rarely reported, while the majority of GBS isolates carry the tetracycline resistance gene tet(M) in fixed genomic positions amongst five predominant clonal clades. In the UK, GBS resistance to clindamycin and erythromycin has increased from 3% in 1991 to 11.9% (clindamycin) and 20.2% (erythromycin), as reported in this study. Here, a systematic investigation of antimicrobial resistance genomic content sought to fully characterise the associated mobile genetic elements within phenotypically resistant GBS isolates from 193 invasive and non-invasive infections of UK adult patients collected during 2014 and 2015. Resistance to erythromycin and clindamycin was mediated by erm(A) (16/193, 8.2%), erm(B) (16/193, 8.2%), mef(A)/msr(D) (10/193, 5.1%), lsa(C) (3/193, 1.5%), lnu(C) (1/193, 0.5%), and erm(T) (1/193, 0.5%) genes. The integrative conjugative elements (ICEs) carrying these genes were occasionally found in combination with high gentamicin resistance mediating genes aac(6′)-aph(2″), aminoglycoside resistance genes (ant(6-Ia), aph(3′-III), and/or aad(E)), alternative tetracycline resistance genes (tet(O) and tet(S)), and/or chloramphenicol resistance gene cat(Q), mediating resistance to multiple classes of antibiotics. This study provides evidence of the retention of previously reported ICESag37 (n = 4), ICESag236 (n = 2), and ICESpy009 (n = 3), as well as the definition of sixteen novel ICEs and three novel transposons within the GBS lineage, with no evidence of horizontal transfer.

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“…However, the resistance to erythromycin and clindamycin has increased globally from 13–20% to 25–32% over the past 20 years as seen by the reports published previously [10] . A study done in the USA concluded that the resistance rates of erythromycin and clindamycin were 54% and 33%, respectively [22] .…”

Section: Discussionmentioning

confidence: 97%

Antibiotic Sensitivity of Group B Streptococcus from Pregnant Mothers and Its Association with Resistance Genes

Dilrukshi¹,

Kottahachchi²,

Dissanayake³

et al. 2023

Med Princ Pract

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Objective This study assessed the antibiotic susceptibility and characterized antibiotic resistance genes of GBS isolates from selected tertiary care hospitals in Western province, Sri Lanka Methods A descriptive cross-sectional study was carried to determine antibiotic sensitivity of GBS among 175 pregnant women of >35 weeks of gestation attending antenatal clinics in four teaching hospitals. Low vaginal and rectal swabs were collected separately, GBS was identified by standard microbiological methods. Antibiotic sensitivity and minimum inhibitory concentration were done according to CLSI guidelines. DNA was extracted from culture isolates and resistance mechanisms were identified by PCR using ermB, ermTR, mefA and linB genes. Results GBS colonization in study sample was 25.7% (45/175) with detection rate of 22.9% and (40/175) and 2.9% (5/175) in vaginal and rectal samples respectively. All isolates were susceptible to penicillin with a MIC range of 0.03-0.12μg/ml. Seventeen (37.7%) were non-susceptible to erythromycin while 6 were intermediate and 11 were resistant. There were 15 (33.3%) non-susceptible isolates for clindamycin with 5 intermediate and ten resistant isolates. Of them seven had inducible clindamycin resistance (iMLSB). Erythromycin and clindamycin MICs ranged 0.03–0.32 μg/ml and 0.06–0.32 μg/ml respectively. The ermB gene was detected in 7(15.5%). The ermTR was found in 16 (35.6%) was significantly associated with iMLSB phenotype (P = 0.005). The mefA gene was detected in two (4.4%) isolates. The linB gene was not detected in tested isolates. Conclusion All isolates were sensitive to penicillin and the most prevalent resistance genotype was ermTR in the study population.

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Survey of strain distribution and antibiotic resistance pattern of group B streptococci (Streptococcus agalactiae) isolated from clinical specimens

Cited by 13 publications

References 24 publications

Antibiotic Susceptibility Patterns and Prevalence of Streptococcus Agalactiae Rectovaginal Colonization Among Pregnant Women in Iran

Antibiotic Susceptibility Patterns and Prevalence of Streptococcus Agalactiae Rectovaginal Colonization Among Pregnant Women in Iran

Genomic Analysis Reveals New Integrative Conjugal Elements and Transposons in GBS Conferring Antimicrobial Resistance

Antibiotic Sensitivity of Group B Streptococcus from Pregnant Mothers and Its Association with Resistance Genes

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