Survey of the Allelic Frequency of a NOS2A Promoter Microsatellite in Human Populations: Assessment of the NOS2A Gene and Predisposition to Infectious Disease

Genetic determinants of rheumatoid arthritis: the inducible nitric oxide synthase (NOS2) gene promoter polymorphism

“…10 Highly significant differences have been reported in the CCTTT allele frequencies between ethnically diverse populations. 15 The observed frequencies of this marker in our cohort of 199 healthy subjects from southern Spain fell within the Caucasian pattern 15 showing 11 different alleles (repeat and bp ranges 7-17 and 171-221 respectively) in unimodal distribution with the peak at the 12 repeat allele (Figure 1). When we compared the distribution of the CCCTT repeat within the control subjects with that of 152 RA patients, both 10 and 15 repeat alleles (186 and 211 bp) showed a trend for increased frequency in the patients group (P = 0.01, OR 1.69, 95% CI 1.07-2.65 and P = 0.03, OR 2.52, 95% CI 0.99-6.57 respectively) that turned out nonsignificant after applying multiple testing (Pc = NS for both alleles).…”

Microsatellite polymorphism in intron 2 of human Toll-like receptor 2 gene is associated with susceptibility to acute pancreatitis in Japan

“…The racial/ethnic differences in allele prevalence of GT repeats agree with other reports showing racial/ethnic differences in microsatellite polymorphisms [36,37].…”

Linkage and association with the NOS2A locus on chromosome 17q11 in multiple sclerosis

“…NOS2 is situated in an important biological checkpoint, and its involvement in disease susceptibility also may go beyond autoimmune demyelination. Linkage in a subset of type I diabetes pedigrees (HLA-DR3/4-positives) 44 and genetic associations have been reported in diabetic renopathy, 45 dementia with Lewy bodies, 20 coronary artery disease, 21 and malaria. 46,47 The NOS2A locus comprises 27 exons with translation initiation and termination codons in exons 2 and 27, respectively.…”

“…The primer sequences for each polymorphism are reported elsewhere. 20,21 Exon 16 C/T SNP genotyping was performed using singlebase extension methodology with specific oligoprobes. Samples were electrophoresed in an ABI377 instrument and analyzed with GeneScan software.…”