Survey of the Apoptotic Effect of Ginnalin A on Hep3b Human Hepatocellular Carcinoma Cell Line

Özden, Pınar; Avcı, Ebru; Vural, Hasibe Cıngıllı

doi:10.3390/proceedings1101064

Cited by 4 publications

(3 citation statements)

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“…Recently, the phenolic compound ginnalin A displayed an apoptotic effect on human hepatocellular carcinoma cell line (Hep3B). Additionally, it showed a promising preventive activity against colon cancer cells (HCT116, SW480, and SW620) with IC 50 values of 24.8 µM, 22.0 µM, and 39.7 µM, respectively [47,58].…”

Section: Assaymentioning

confidence: 99%

UHPLC–Q/Orbitrap/MS/MS Fingerprinting, Free Radical Scavenging, and Antimicrobial Activity of Tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) Lyophilized Decoction from Argentina and Chile

et al. 2019

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The decoction of Tessaria absinthioides is used in traditional medicine of South America as hypocholesterolemic, balsamic, and expectorant; but it is also useful for the prevention of hepatitis, renal insufficiency, and diabetes, and is used as digestive. A lyophilized decoction from the aerial parts of this plant (TLD) collected in San Juan (TLDSJ) and Mendoza (TLDM) provinces (Argentina) and one collection from Antofagasta, Chile (TLDCH) were characterized regarding antioxidant and antibacterial activities, phenolics and flavonoids content, and ultrahigh resolution liquid chromatography Orbitrap MS analysis UHPLC–PDA–OT-MS/MS metabolite profiling. The antioxidant properties were carried out "in vitro" using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and trolox equivalent antioxidant activity (TEAC) methods, ferric-reducing antioxidant power (FRAP), and lipoperoxidation in erythrocytes (LP). The antibacterial activity was evaluated following the Clinical and Laboratory Standards Institute (CLSI) rules. TLDSJ, TLDM, and TLDCH displayed a strong DPPH scavenging activity (EC50 = 42, 41.6, and 43 µg/mL, respectively) and inhibition of lipoperoxidation in erythrocytes (86–88% at 250 µg TLD/mL), while a less effect in the FRAP and TEACantioxidant assays was found. Additionally, the decoctions showed a content of phenolics compounds of 94 mg gallic acid equivalents (GAE)/g, 185 GAE/g, and 64 GAE/g, for TLDSJ, TLDM, and TLDCH samples, respectively. Regarding the flavonoid content, the Chilean sample was highlighted with 19 mg quercetin equivalents (QE)/g. In this work, several phenolic compounds, including sesquiterpenes, flavonoids, and phenolic acids, were rapidly identified in TLDSJ, TLDM, and TLDCH extracts by means UHPLC–PDA–OT-MS/MS for the first time, which gave a first scientific support to consider this medicinal decoction from both countries as a valuable source of metabolites with antioxidant effects, some with outstanding potential to improve human health.

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Section: Assaymentioning

confidence: 99%

UHPLC–Q/Orbitrap/MS/MS Fingerprinting, Free Radical Scavenging, and Antimicrobial Activity of Tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) Lyophilized Decoction from Argentina and Chile

et al. 2019

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“…It also showed some cytotoxicity against L1210, HL-60, K562, and B16F10 cancer cell lines in vitro by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay [ 11 ]. Ginnalin A inhibited the proliferation of HCT-116 and MCF-7 cell lines and it also obstructed the S and G2/M-phases of the cell cycle by down-regulating the levels of cyclin A and cyclin D1 [ 21 ]. Ginnalin A inhibited the proliferation of HCT-116, HT-29, and Caco-2 cell lines by blocking the S-phase of the cell cycle [ 22 ].…”

Section: Therapeutic Potential Of Ginnalin a And Hammentioning

confidence: 99%

Ginnalin A and hamamelitannin: the unique gallotannins with promising anti-carcinogenic potential

Rippin

Beniwal

Sharma

et al. 2023

Exploration of Targeted Anti-Tumor Therapy

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Tannins are secondary metabolites that belong to the family of polyphenolic compounds and have gained a huge interest among researchers due to their versatile therapeutic potential. After lignin, these are the second most abundant polyphenols found in almost every plant part like stem, bark, fruit, seed, leaves, etc. Depending upon their structural composition, these polyphenols can be divided into two distinct groups, namely condensed tannins and hydrolysable tannins. Hydrolysable tannins can be further divided into two types: gallotannins and ellagitannins. Gallotannins are formed by the esterification of D-glucose hydroxyl groups with gallic acid. The gallolyl moieties are bound by a depside bond. The current review focuses mainly on the anti-carcinogenic potential of recently discovered gallotannins, ginnalin A, and hamamelitannin (HAM). Both of these gallotannins possess two galloyl moieties linked to a core monosaccharide having anti-oxidant, anti-inflammatory, and anti-carcinogenic abilities. Ginnalin A is found in plants of the genus Acer whereas HAM is present in witch hazel plants. The biosynthetic pathway of ginnalin A along with the mechanism of the anti-cancer therapeutic potential of ginnalin A and HAM has been discussed. This review will certainly help researchers to work further on the chemo-therapeutic abilities of these two unique gallotannins.

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“…Hepatocellular carcinoma is one of the main reasons for cancer deaths worldwide [ 12 ]. Özden et al [ 50 ] investigated the apoptotic impact of Ginnalin A in a human hepatocellular carcinoma cell line (Hep3B). IC 50 of Ginnalin A was calculated to be 155 μM in Hep3B cells for 72h.…”

Section: Nutritional and Functional Properties Of Maple Syrupmentioning

confidence: 99%

Nutritional, pharmacological, and sensory properties of maple syrup: A comprehensive review

Mohammed,

Sibley,

Abdulwali

et al. 2023

Heliyon

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Survey of the Apoptotic Effect of Ginnalin A on Hep3b Human Hepatocellular Carcinoma Cell Line

Cited by 4 publications

References 0 publications

UHPLC–Q/Orbitrap/MS/MS Fingerprinting, Free Radical Scavenging, and Antimicrobial Activity of Tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) Lyophilized Decoction from Argentina and Chile

UHPLC–Q/Orbitrap/MS/MS Fingerprinting, Free Radical Scavenging, and Antimicrobial Activity of Tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) Lyophilized Decoction from Argentina and Chile

Ginnalin A and hamamelitannin: the unique gallotannins with promising anti-carcinogenic potential

Nutritional, pharmacological, and sensory properties of maple syrup: A comprehensive review

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