Survey of the fatty acid composition of Canadian beef: Backfat and longissimus lumborum muscle

Aldai, Noelia; Dugan, M. E. R.; Rolland, D. C.; Kramer, J. K. G.

doi:10.4141/cjas08126

Cited by 68 publications

(61 citation statements)

References 68 publications

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“…For FA analysis, 50 mg fat was freeze dried and directly methylated with 0.5 M sodium methoxide (Aldai et al, 2009). Fatty acid methyl esters were analysed by gas chromatography (GC) using a CP-Sil88 column (100 m, 25 µm ID, 0.2 µm film thickness) in a CP-3800 gas chromatograph equipped with an 8600-series autosampler (Varian Inc., Walnut Creek, Calif, USA).…”

Section: Methodsmentioning

confidence: 99%

Fatty acid composition of beef steers as affected by diet and fat depot

Mapiye¹,

Vahmani²,

Aalhus³

et al. 2015

SA J. An. Sci.

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Subcutaneous and perirenal fatty acid (FA) profiles were compared in steers fed a control diet (70 : 30 red clover silage (RC) : barley concentrate), a diet with sunflower seed (SS) substituted for barley, and diets with 15% or 30% wheat dried distillers' grain with solubles (DDGS-15 and DDGS-30) substituted for RC and SS. Perirenal fat (PRF) versus subcutaneous fat (SCF) had greater proportions of total saturated FA (SFA) and branched chain FA (BCFA), and lower proportions of total and major cis-monounsaturated FA (c-MUFA). Addition of SS to the diet did not change the proportions of total and major c-MUFA and n-6 polyunsaturated FA (PUFA), but led to decreases in the proportions of total and major SFA, BCFA and n-3 PUFA. Progressive substitutions with DDGS led to no further changes in the proportions of total and major SFA and n-3 PUFA, but decreased the proportions of BCFA and c9-16:1, and increased the proportions of c9-18:1 and n-6 PUFA. Feeding SS and DDGS-15 diets yielded the largest proportions of total and major t-18:1 (t11-and t13-/t14-18:1) isomers in PRF and conjugated lineolic acid (CLA) isomers (t7,c9-and t9,c11-18:2) in SCF, but responses were diminished when feeding the DDGS-30 diet. Subcutaneous fat versus PRF from steers fed SS and DGGS diets had larger proportions of non-conjugated 18:2 biohydrogenation products (i.e. atypical dienes) than the control diet. Overall, feeding SS and DDGS-15 diets raised the proportions of t11-18:1 in PRF and c9,t11-18:2 in SCF, which have potential human health benefits, but feeding DDGS-30 was less effective.

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Section: Methodsmentioning

confidence: 99%

Fatty acid composition of beef steers as affected by diet and fat depot

Mapiye¹,

Vahmani²,

Aalhus³

et al. 2015

SA J. An. Sci.

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“…Grain-fed beef in North America has been surveyed and shown to have more trans t10-18:1 than t11-18:1 (vaccenic acid; Aldai et al, 2009). This is attributed to feeding high levels of small grains like barley or wheat with rapidly fermentable starch, which causes rumen pH depression and a shift in the rumen bacterial population from vaccenic acid to t10-18:1 producing species (Zened et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Inclusion of sunflower seed and wheat dried distillers’ grains with solubles in a red clover silage-based diet enhances steers performance, meat quality and fatty acid profiles

Mapiye

Aalhus

Turner

et al. 2014

Animal

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The current study compared beef production, quality and fatty acid (FA) profiles of yearling steers fed a control diet containing 70 : 30 red clover silage (RCS) : barley-based concentrate, a diet containing 11% sunflower seed (SS) substituted for barley, and diets containing SS with15% or 30% wheat dried distillers' grain with solubles (DDGS). Additions of DDGS were balanced by reductions in RCS and SS to maintain crude fat levels in diets. A total of two pens of eight animals were fed per diet for an average period of 208 days. Relative to the control diet, feeding the SS diet increased ( P < 0.05) average daily gain, final live weight and proportions of total n-6 FA, non-conjugated 18:2 biohydrogenation products (i.e. atypical dienes) with the first double bond at carbon 8 or 9 from the carboxyl end, conjugated linoleic acid isomers with the first double bond from carbon 7 to 10 from the carboxyl end, t-18:1 isomers, and reduced ( P < 0.05) the proportions of total n-3 FA, conjugated linolenic acids, branched-chain FA, odd-chain FA and 16:0. Feeding DDGS-15 and DDGS-30 diets v. the SS diet further increased ( P < 0.05) average daily gains, final live weight, carcass weight, hot dressing percentage, fat thickness, rib-eye muscle area, and improved instrumental and sensory panel meat tenderness. However, in general feeding DGGS-15 or DDGS-30 diets did not change FA proportions relative to feeding the SS diet. Overall, adding SS to a RCS-based diet enhanced muscle proportions of 18:2n-6 biohydrogenation products, and further substitutions of DDGS in the diet improved beef production, and quality while maintaining proportions of potentially functional bioactive FA including vaccenic and rumenic acids.

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“…Details of this procedure have been published elsewhere (Aldai, Dugan, Rolland, & Kramer, 2009). Lipid aliquots (~10 mg) from each steak were methylated separately using acidic (methanolic HCl) and basic (sodium methoxide) reagents to ensure complete methylation of all lipids and avoid isomerisation of conjugated linoleic acids (CLAs), respectively (Aldai, Murray, Nájera, Troy, & Osoro, 2005; Kramer, Hernandez, Cruz-Hernandez, Kraft, & Dugan, 2008).…”

Section: Fatty Acid Analysismentioning

confidence: 99%