Survey of the year 2000 commercial optical biosensor literature

Rich, Rebecca L.; Myszka, David G.

doi:10.1002/jmr.547

Cited by 82 publications

(36 citation statements)

References 597 publications

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“…Comprehensive lists of recently published articles describing works employing commercial biosensors and emerging applications in areas of drug discovery, clinical support, food and environment monitoring, and cell membrane biology are available (Rich and Myszka, 2001). In addition, reviews outlining the currently available biosensor hardware and highlighting unique features of each platform have been published recently (Baird and Myszka, 2001;Iqbal et al, 2000;Polla et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…The development of advanced analytical and bioseparation methodologies based on micro-or macroarrays and biosensors is one of the strategic objectives of the so-called postgenomics, but it also has an impact on strategic application fields, such as predictive oncology, diagnostics in the biomedical field, and drug research (Gastrock et al, 2001;Haake et al, 2000;McGlennen, 2001;Rich and Myszka, 2001;Van Regenmortel, 2001). Comprehensive lists of recently published articles describing works employing commercial biosensors and emerging applications in areas of drug discovery, clinical support, food and environment monitoring, and cell membrane biology are available (Rich and Myszka, 2001).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Levitation and movement of human tumor cells using a printed circuit board device based on software‐controlled dielectrophoresis

Altomare¹,

Borgatti²,

Medoro³

et al. 2003

Biotech & Bioengineering

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In this study we describe an original, efficient, and innovative printed circuit board (PCB) device able to generate dielectrophoresis-based, software-controlled cages that can be moved to any place inside a microchamber. Depending on their dielectrophoretic properties, eukaryotic cells can be "entrapped" in cages and moved under software control. The main conclusion gathered from the experimental data reported is that the PCB device based on dielectrophoresis permits levitation and movement of different tumor cells at different dielectrophoresis conditions. The results presented herein are therefore the basis for experiments aimed at forced interactions or separation of eukaryotic cells using "lab-on-a-chip." In fact, because many cages can be controlled at the same time, and two or more cages can be forced to share the same or a different location, it is possible, in principle, either to bring in contact cells of a differing histotype or to separate them.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Levitation and movement of human tumor cells using a printed circuit board device based on software‐controlled dielectrophoresis

Altomare¹,

Borgatti²,

Medoro³

et al. 2003

Biotech & Bioengineering

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“…[1,2] Major applications have been reported, not only for protein-protein interactions, including in conjunction with mass spectrometry, but also in SPR studies on nucleic acid-protein, carbohydrateprotein, and carbohydrate-carbohydrate interactions. [3][4][5][6] Qualitative SPR applications range from orphan-ligand and small-analyte screening to epitope mapping and complex assembly studies, whereas quantitative experiments include concentration measurements of active molecules in solution, evaluation of competition/inhibition events, and determination of rate and affinity constants. Nevertheless, since the SPR response is proportional to the accumulation of mass on the sensor surface, a serious constraint imposed by this technique concerns the dimensions of the molecules to be employed as analytes.…”

Section: Introductionmentioning

confidence: 99%

SPR Studies of Carbohydrate–Protein Interactions: Signal Enhancement of Low‐Molecular‐Mass Analytes by Organoplatinum(II)‐Labeling

et al. 2005

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The relatively insensitive surface plasmon resonance (SPR) signal detection of low‐molecular‐mass analytes that bind with weak affinity to a protein—for example, carbohydrate–lectin binding—is hampering the use of biosensors in interaction studies. In this investigation, low‐molecular‐mass carbohydrates have been labeled with an organoplatinum(II) complex of the type [PtCl(NCNR)]. The attachment of this complex increased the SPR response tremendously and allowed the detection of binding events between monosaccharides and lectins at very low analyte concentrations. The platinum atom inside the organoplatinum(II) complex was shown to be essential for the SPR‐signal enhancement. The organoplatinum(II) complex did not influence the specificity of the biological interaction, but both the signal enhancement and the different binding character of labeled compounds when compared with unlabeled ones makes the method unsuitable for the direct calculation of biologically relevant kinetic parameters. However, the labeling procedure is expected to be of high relevance for qualitative binding studies and relative affinity ranking of small molecules (not restricted only to carbohydrates) to receptors, a process of immense interest in pharmaceutical research.

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“…We can defend this statement because we read the literatureevery single article containing commercial biosensor results that has been published -and for the past decade we have written an annual review of the year's literature (13)(14)(15)(16)(17)(18)(19)(20)(21)(22). Most often the problems arise from poor experimental design and execution, as well as inadequate or inappropriate data analysis.…”

Section: Spr Pessimistsmentioning

confidence: 99%

“…15 Example shapes of published responses. The shape in (A) depicts a plausible binding response, while those in (B) are due to experimental artifacts.…”

mentioning

confidence: 99%