Survey of Theileria Parasite Infection in Cattle in Taiwan.

Wang, Chun-Tshen; Kubota, Shuichi; Kakuda, Tsutomu; Kuo, Chin‐Chi; Hsu, Tien-Lai; Onuma, Misao

doi:10.1292/jvms.60.253

Cited by 15 publications

(11 citation statements)

References 8 publications

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“…Previously, one Okinawa isolate exhibited an SDS-PAGE protein profile similar to that of T. buffeli (Zakimi et al, unpublished data). According to epidemiological studies, I-type, rather than C-and Btype parasites, tends to be associated with clinical theileriosis [9,21]. However, no obvious correlations between MPSP types and clinical signs as measured by PCV were evident in this study, (data not shown).…”

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confidence: 66%

Genetic Diversity of Benign Theileria Parasites of Cattle in the Okinawa Prefecture

Zakimi

Kim

Oshiro

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. Benign Theileria parasites of cattle distributed in the Okinawa prefecture were characterized by allele-specific polymerase chain reaction (PCR) and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. Using universal or allele-specific primer sets, parasite DNA was amplified in 31 out of 48 blood samples obtained from beef cattle. Among the positive cases, mixed infections involving various combinations of I-, C-, and B-type parasites were detected in 24 (77.4%) samples. Phylogenetic analysis based on the MPSP gene sequences revealed that parasites with the MPSP types 1-5 and 7, exist within the Okinawa prefecture.

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confidence: 66%

Genetic Diversity of Benign Theileria Parasites of Cattle in the Okinawa Prefecture

Zakimi

Kim

Oshiro

et al. 2006

The Journal of Veterinary Medical Science

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“…Clinical infection can also result in decreased milk production in dairy cattle (7). T. orientalis is a conditional pathogen, and while pathogenic forms are largely limited to Eastern Asia and Australasia (5, 6, 8-10), it is frequently detected in asymptomatic animals; these benign forms are globally spread (5,(11)(12)(13)(14). Analysis of the most common genotyping locus (p32), encoding the variable major piroplasm surface protein (MPSP), currently identifies 11 distinct T. orientalis genotypes (13,15,16).…”

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confidence: 99%

Development and Validation of a Quantitative PCR Assay Using Multiplexed Hydrolysis Probes for Detection and Quantification of Theileria orientalis Isolates and Differentiation of Clinically Relevant Subtypes

et al. 2015

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T heileria orientalis is a vector-borne hemoprotozoan that infects cattle and buffalo and is generally spread by ticks of the Haemaphysalis genus (1-3). Historically, this organism has been referred to as Theileria sergenti, Theileria buffeli, or the T. orientalis/T. sergenti/T. buffeli complex; however, the name T. sergenti is now considered invalid (4), and T. orientalis is commonly used to refer to all (5). From a clinical perspective, T. orientalis can cause anemia, lethargy, jaundice, fever, abortion, and mortality in cattle (6). Clinical infection can also result in decreased milk production in dairy cattle (7). T. orientalis is a conditional pathogen, and while pathogenic forms are largely limited to Eastern Asia and Australasia (5, 6, 8-10), it is frequently detected in asymptomatic animals; these benign forms are globally spread (5,(11)(12)(13)(14). Analysis of the most common genotyping locus (p32), encoding the variable major piroplasm surface protein (MPSP), currently identifies 11 distinct T. orientalis genotypes (13,15,16). Of these genotypes, type 2 (Ikeda) and to a lesser extent type 1 (Chitose) are typically found in association with clinical disease (6,9,10,(17)(18)(19)(20)(21)(22). The presence of pathogenic and benign forms of T. orientalis greatly complicates its clinical diagnosis, with standard blood film analysis unable to identify the pathogenic genotypes.Multiple conventional PCR (cPCR) assays have been published for the identification of T. orientalis in blood samples (9,21,23,24). The most commonly cited assays detect and genotype T. orientalis by amplifying unique regions of the MPSP gene (21). These assays use two universal primers to detect T. orientalis infection and specific forward primers to identify the Ikeda, Chitose, and Buffeli genotypes. While this method is highly sensitive and has been validated in prior studies (19,21), it is not multiplexed and is highly susceptible to PCR inhibitors (6), which are often found in nucleotide extractions obtained from blood (25,26). To overcome inhibition, undiluted and diluted nucleotide extracts can be examined in parallel to prevent false-negative results (6,19,27). However, if multiple reactions per sample are required to determine the presence of infection, the procedure becomes both expensive and time-consuming. Furthermore, cPCR does not give an accurate representation of parasite load and therefore cannot provide an indication of the severity of T. orientalis infection. A recently developed multiplexed tandem PCR (28) is able to discriminate between four genotypes of T. orientalis, but it is only semiquantitative and therefore cannot be used to define a clinical threshold.Hydrolysis probe quantitative PCR (qPCR) assays employ sequence-specific, fluorescently labeled probes attached to duplex-

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“…The minority (14/28) was C-type parasites which were found as a mixed population with Thai-and B1-type parasites. Similarly, the Australian Italian and Taiwan benign Theileria parasites are also composed of B1-and C-type [17,23]. Nucleotide sequences of Australian C-type MPSP gene was similar to that of Japanese C-type parasite with 98.5% homology [11].…”

Section: Resultsmentioning

confidence: 99%

Genetic Diversity of Major Piroplasm Surface Protein Genes and Their Allelic Variants of Theileria Parasites in Thai Cattle.

Sarataphan¹,

Nilwarangkoon

Tananyutthawongese

et al. 1999

The Journal of Veterinary Medical Science

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ABSTRACT. Twenty-eight field isolated Theileria parasite DNAs obtained from dairy and beef cattle in distinct geographical areas of Thailand were characterized by using polymerase chain reaction (PCR) amplification with six sets of oligonucleotide primers. Three sets of them were modified from two genes of immunodominant major piroplasm surface protein (MPSP) coding for 32 kDa (p32) of T. sergenti and 33/34 kDa (p33/34) of T. buffeli, and MPSP of Theileria spp.(Thai-isolate). The other three sets of primers were basically generated from three alleles of MPSP which were specific for Japanese T. sergenti-Ikeda stock (I-type), Japanese T. sergenti-Chitose stock (C-type) and Australian T. buffeli-Warwick stock (B1-type), respectively. The results indicated that 14 out of 28 isolates were amplified by the Thai-specific primer whereas 6 isolates were amplified by the p32 specific primer and the other 5 isolates were amplified by the p32 and Thai-specific primers. In addition, by using the allele-specific PCR, 14 out of 28 isolates contained C-type MPSP whereas 3 isolates contained B1 type parasites. Interestingly, 20 out of 28 isolates could be amplified by the Thai-specific primer. The majority of Theileria parasites distributed in Thailand contained Thai type parasites, whereas C-type parasites showed the mixed population with B1 and Thai type parasites. No I type parasite was detected.-KEY WORDS: major piroplasm surface protein, PCR, Theileria.J. Vet. Med. Sci. 61(9): 991-994, 1999 well as B1-type MPSP allele. This study revealed that T.

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Survey of Theileria Parasite Infection in Cattle in Taiwan.

Cited by 15 publications

References 8 publications

Genetic Diversity of Benign Theileria Parasites of Cattle in the Okinawa Prefecture

Genetic Diversity of Benign Theileria Parasites of Cattle in the Okinawa Prefecture

Development and Validation of a Quantitative PCR Assay Using Multiplexed Hydrolysis Probes for Detection and Quantification of Theileria orientalis Isolates and Differentiation of Clinically Relevant Subtypes

Genetic Diversity of Major Piroplasm Surface Protein Genes and Their Allelic Variants of Theileria Parasites in Thai Cattle.

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