Survey on Actual Pest Management Condition of Persimmon Orchards in Sancheong, Gyeongsangnam-do and Yeongdong, Chungcheongbuk-do

Yu, Hwang Bin; Lim, Tae Heon; Jung, Mun Gi; Kim, Jung Eun; Park, Chang Je; Song, Jiazheng; Lee, Dong Woon

doi:10.7585/kjps.2017.21.4.417

Cited by 4 publications

(1 citation statement)

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“…Wild species in their natural habitat have greater genetic diversity without genetic bottlenecks and genetic uniformity and have been used for more than 100 years to increase resistance to pests and diseases (Mujeeb-Kazi and Kimber 1985). Despite the importance of wild plant species, studies have only been conducted on major cultivated crops in Korea (Yu et al 2017).…”

Section: Development Of One-step Mrt-pcr and Validationmentioning

confidence: 99%

A survey of viruses and viroids in astringent persimmon (Diospyros kakiThunb.) and the development of a one-step multiplex reverse transcription-polymerase chain reaction assay for the identification of pathogens

et al. 2022

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Astringent persimmon (Diospyros kaki Thunb.) is an important fruit crop in Korea; it possesses significant medicinal potential. However, knowledge regarding the pathogens affecting this crop, particularly, viruses and viroids, is limited. In the present study, reverse transcriptionpolymerase chain reaction (RT-PCR) and high-throughput transcriptome sequencing (HTS) were used to investigate the viruses and viroids infecting astringent persimmons cultivated in Korea. A one-step multiplex RT-PCR (mRT-PCR) method for the simultaneous detection of the pathogens was developed by designing species-specific primers and selecting the primer pairs via combination and detection limit testing. Seven of the sixteen cultivars tested were found to be infection-free. The RT-PCR and HTS analyses identified two viruses and one viroid in the infected samples (n = 51/100 samples collected from 16 cultivars). The incidence of single infections (n = 39/51) was higher than that of mixed infections (n = 12/51); the infection rate of the Persimmon cryptic virus was the highest (n = 31/39). Comparison of the monoplex and mRT-PCR results using randomly selected samples confirmed the efficiency of mRT-PCR for the identification of pathogens. Collectively, the present study provides useful resources for developing disease-free seedlings; further, the developed mRT-PCR method can be extended to investigate pathogens in other woody plants.

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Section: Development Of One-step Mrt-pcr and Validationmentioning

confidence: 99%