Survey on antimicrobial resistance and virulence-related genes in Enterococcus faecium and Enterococcus faecalis collected from hospital environment in the north of Iran

Marghmalek, Saba Asgharzadeh; Valadan, Reza; Gholami, Mehrdad; Nasrolahei, Mohtaram; Goli, Hamid Reza

doi:10.1016/j.genrep.2021.101233

Cited by 4 publications

(5 citation statements)

References 33 publications

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“…Amongst the erm genes detected, ermB was the most prevalent gene in erythromycin resistant Enterococcus faecalis isolates. This finding concurred with the findings of earlier studies (Ahmadpoor , et al, 2021;Marghmalek, et al, 2021;Kim, et al, 2019;Tian, et al, 2019). tetM was the most frequently detected gene among the tetracycline resistant Enterococcus faecalis isolates.…”

Section: Discussionsupporting

confidence: 92%

Antimicrobial Resistance Profiles of Enterococcus Faecalis and Enterococcus Faecium Isolated from Clinical Specimens at Kitwe Teaching Hospital in Zambia

et al. 2024

IJFMR

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This research examined the occurrence of Enterococcus faecalis and Enterococcus faecium in clinical samples processed at Kitwe Teaching Hospital Laboratory, along with their resistance to antimicrobials and presence of virulence determinants. A total of 230 clinical specimens were analyzed to identify suspected Enterococcus strains. Standard laboratory techniques were employed to isolate, characterize, and confirm the species of Enterococcus, followed by testing their susceptibility to antimicrobials and detection of resistance and virulence genes using polymerase chain reaction. Among the 230 cultured specimens, 89 were stool samples, 3 were swabs, and 138 were urine specimens. Out of these, 71 isolates were confirmed Enterococcus by PCR using genus specific primers. The resulting prevalence rate of 30.9% was thus obtained. The most prevalent species was Enterococcus faecalis, accounting for 56.3% of the 71 Enterococcus isolates. The prevalence of E. faecium was 14.1%. All tested E. faecalis isolates displayed resistance to ciprofloxacin and erythromycin. Furthermore, high rates of resistance were observed among E. faecalis isolates for chloramphenicol and tetracycline (97.5%), vancomycin (90.0%), nitrofurantoin (75.0%), penicillin (75.0%) and ampicillin (67.5%). Only two E. faecium isolates exhibited susceptibility to tetracycline and vancomycin, while the remaining isolates were 100% resistant to all other tested antimicrobials. Multidrug resistance was detected in all E. faecium isolates. In addition, various antibiotic resistance genes (aac(6′)-Ie-aph(2″)-LA, ermA, ermB, tetK, tetL, tetM, tetX, vanA) were identified in both E. faecalis and E. faecium isolates. A positive association between phenotype and genotype was found for tetracycline and erythromycin.

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Section: Discussionsupporting

confidence: 92%

Antimicrobial Resistance Profiles of Enterococcus Faecalis and Enterococcus Faecium Isolated from Clinical Specimens at Kitwe Teaching Hospital in Zambia

et al. 2024

IJFMR

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“…It should be noted that E. faecalis and E. faecium cause nosocomial infections in humans (Asgharzadeh Marghmalek et al ., 2021; Souillard et al ., 2022). Moreover, other enterococci such as E. casseliflavus can also be pathogenic to humans (Yoshino, 2023).…”

Section: Resultsmentioning

confidence: 99%

“…In addition, we isolated E. devriesei, E. gilvus and E. hirae from pork meat samples. However, other researchers have isolated other species such as E. durans, E. gallinarum, E. raffinosus, (Asgharzadeh Marghmalek et al, 2021;Souillard et al, 2022). Moreover, other enterococci such as E. casseliflavus can also be pathogenic to humans (Yoshino, 2023).…”

Section: Enumeration and Prevalence Of Enterococci Isolated From Pork...mentioning

confidence: 99%

“…Enterococci are used as stool indicator microorganisms (Byappanahalli et al ., 2012). On the other hand, enterococci are very important opportunistic bacteria that cause a range of infections (Asgharzadeh Marghmalek et al ., 2021; Souillard et al ., 2022). As they have the ability to survive in adverse ambient conditions, these bacteria appear in nosocomial diseases with increasing frequency.…”

Section: Introductionmentioning

confidence: 99%

“…As they have the ability to survive in adverse ambient conditions, these bacteria appear in nosocomial diseases with increasing frequency. Enterococcus fecalis and Enterococcus faecium cause common infections in humans mainly in hospitals (Asgharzadeh Marghmalek et al ., 2021). Most of the studies on enterococci in poultry meat have been carried out in chicken and turkey meat, while no information is available in duck and quail meat (Hayes et al ., 2003; McGowan et al ., 2006; Kročko et al ., 2011; Maasjost et al ., 2015; Tyson et al ., 2018; Manson et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterisation of antimicrobial resistance of Enterococcus spp. isolated from pork and poultry meat

Martínez‐Laorden

Arraiz‐Fernandez

González‐Fandos

2023

Int J of Food Sci Tech

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There is a great concern on the presence of antimicrobial-resistant bacteria in meat, since they can cause adverse effects in the consumers' health. The purpose of this work was to identify and characterise the antimicrobial resistance of Enterococcus spp. isolated from meat. A total of 209 meat samples (forty pork, fifty chicken, fifty-one turkey, thirty-one duck, and thirty-seven quail) were collected at retail level. E. faecalis was the dominant enterococci isolated from all the types of meat evaluated, followed by E. faecium, E. gallinarum, and E. hirae, among others which were also isolated. Of the 317 strains evaluated for antimicrobial resistance 48 (15.14%) were multi-resistant. Most of the multi-resistant strains were E. faecium and E. faecalis, and in lesser extent E. gallinarum and E. hirae. Multi-resistant strains were isolated from all the types of meat studied, mainly from turkey (sixteen strains), chicken (fifteen strains), and quail (fourteen strains). Two multi-resistant strains showed resistance to vancomycin (E. faecium isolated from turkey meat, and E. faecalis isolated from quail meat). Resistance to tigecycline, teicoplanin, linezolid and quinolones was found among enterococci isolates. Special measures should be taken to avoid faecal contamination of carcasses in order to reduce enterococci prevalence in meat.

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Rapid and specific detection of Enterococcus faecium with an isothermal amplification and lateral flow strip combined method

Wang,

Zhang,

Huo

et al. 2023

Arch Microbiol

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Survey on antimicrobial resistance and virulence-related genes in Enterococcus faecium and Enterococcus faecalis collected from hospital environment in the north of Iran

Cited by 4 publications

References 33 publications

Antimicrobial Resistance Profiles of Enterococcus Faecalis and Enterococcus Faecium Isolated from Clinical Specimens at Kitwe Teaching Hospital in Zambia

Antimicrobial Resistance Profiles of Enterococcus Faecalis and Enterococcus Faecium Isolated from Clinical Specimens at Kitwe Teaching Hospital in Zambia

Identification and characterisation of antimicrobial resistance of Enterococcus spp. isolated from pork and poultry meat

Rapid and specific detection of Enterococcus faecium with an isothermal amplification and lateral flow strip combined method

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