This study aims to determine the influence of oxytetracycline and prostaglandin F2α during the puerperium period, on uterine recovery and post-parturient reproductive characteristics in Baladi cows. Twenty Baladi cows were used immediately after birth and were divided into two groups of 10 cows per group. The first group (n= 10 cows) was treated with 20 ml intrauterine infusion of oxytetracycline hydrochloride solution for three consecutive days during the puerperium period and 5ml intramuscular prostaglandin F2α injection once after three hours from calving, while the second group (n= 10 cows) was used as a control group. The present findings demonstrated the interval from parturition to uterine involution in Baladi cows was significantly (P <0.05) lower, (28.4 ± 4.5, days) in treated cows compared to 45.7 ± 3.5, days in control group cows. However, the period from calving to the resumption of estrus activity postpartum was significantly (P <0.05) below, 45.2±9.1, days in treated cows compared to 68.2±6.2, days in control cows. Meanwhile, the time from calving to conceived was significantly (P <0.05) less (96.5±12.3, days) in treated cows than (155.3 ±15.2, days) in controlled cows. In addition, the conception rate from first mating was significantly (P <0.05) higher, 60% in treated cows compared to 30% in control cows. In conclusion, the current study demonstrated improvement in post-partum reproductive performance in cows which treated with oxytetracycline and prostaglandinF2αduring the puerperium period. So, the study recommends that small breeders should treat their cows during the puerperium period.