Survey on diversity of marine/saline anaerobic Heterolobosea (Excavata: Discoba) with description of seven new species

Pánek, Tomáš; Ptáčková, Eliška; Čepička, Ivan

doi:10.1099/ijs.0.063487-0

Cited by 19 publications

(21 citation statements)

References 44 publications

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“…The enigmatic electron‐dense structures that were found in the cytoplasm of the UD12 cells, along with mitochondria with well defined discoid cristae, had no internal membranes and thus looked similar both to the acristate organelles described in anaerobic heteroloboseans (Pánek et al. ) and to some inclusions documented by Fawcett (). Comparison of dozens of ultrathin sections through the globular UD12 cells revealed certain signs of transformation of the organelle in question.…”

Section: Discussionsupporting

confidence: 67%

“…Pánek et al. (, ,b) described “acristate mitochondria (hydrogenosomes)” in obligate heterolobosean anaerobes or microaerophils. For structures similar to hydrogenosomes in which putative cristae could be recognized only exceptionally they coined the term “mitochondrion‐related organelles (MROs)”.…”

Section: Discussionmentioning

confidence: 99%

“…), led Pánek et al. () to consider existence of a complex metabolism in some anaerobic heteroloboseans that they described as new species.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, surprising details of the ultrastructure of microaerophilic or anaerobic heteroloboseans (Pánek and Cepicka ; Pánek et al. ,b) advocate revision of heterolobosean characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…) of immensely diverse protists (Pánek and Cepicka ; Pánek et al. , ). Of those, the most attention has been paid to vahlkampfiids (Vahlkampfiidae Jollos, ).…”

mentioning

confidence: 99%

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Neovahlkampfia nana n. sp. Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea

Tyml

Lares-Jiménez

Kostka

et al. 2016

J Eukaryotic Microbiology

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The study provides robust genetic evidence that a newly isolated naked ameba with morphological and ultrastructural features indicative of Heterolobosea is a new species. Neovahlkampfia nana n. sp. associates with the yet underrepresented subclade of Tetramitia I. Considerable differences found in 18S rRNA gene sequences of individual molecular clones derived from DNA of five clonal cultures, using a low fidelity DNA polymerase, raised the issue of intragenomic sequence variation, a phenomenon that has not been previously studied in Heterolobosea. However, as proved using a higher fidelity DNA polymerase, the sequence variability observed was introduced by PCR mediated by the low fidelity polymerase and fixed by molecular cloning. This points to the potentially dubious validity of some current nominal species of Heterolobosea that differ from one another in just one or two base positions.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

“…), led Pánek et al. () to consider existence of a complex metabolism in some anaerobic heteroloboseans that they described as new species.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…) of immensely diverse protists (Pánek and Cepicka ; Pánek et al. , ). Of those, the most attention has been paid to vahlkampfiids (Vahlkampfiidae Jollos, ).…”

mentioning

confidence: 99%

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