Survival ability of cytotoxic strains of motile Aeromonas spp. in different types of water

Brandi, Giorgio; Sisti, Maurizio; Giardini, Franco; Schiavano, Giuditta Fiorella; Albano, A

doi:10.1046/j.1365-2672.1999.00584.x

Cited by 48 publications

(25 citation statements)

References 18 publications

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“…The initial rapid growth seen with the A. hydrophila aroA strain after inoculation of nonsterilized water microcosms has also been reported for other A. hydrophila strains (10,18) as well as for other species, such as Vibrio vulnificus (11), Pseudomonas aeruginosa (35,69), and Photobacterium damselae subsp. damselae (21), and appears to be correlated with the water volume and the recipient surface (34).…”

Section: Discussionsupporting

confidence: 59%

“…Several studies concerning the survival of A. hydrophila strains in aquatic environments, such as distilled water (44), mineral water (18,45,47), tap water (43), and seawater (40), have been published. Microcosms are often used in these studies, most of them applied to assess the health impact of the presence of motile Aeromonas species in drinking water, because the production of a wide range of virulence factors is a common feature of virulent strains (10,13,53,54,64).…”

Section: Cfu MLmentioning

confidence: 99%

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Behavior of an Aeromonas hydrophila aroA Live Vaccine in Water Microcosms

Vivas¹,

Carracedo²,

Riaño³

et al. 2004

Appl Environ Microbiol

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Genetically modified auxotrophic mutants of different fish pathogens have been used as live vaccines in laboratory experiments, but the behavior of the strains after release into aquatic ecosystems has not been characterized. We previously constructed and characterized an aroA mutant of Aeromonas hydrophila and studied the protection afforded by this mutant as a live vaccine in rainbow trout. In this work, we describe the survival of this strain in aquatic microcosms prepared from fish water tanks. The aroA mutant disappeared rapidly in nonfiltered, nonautoclaved fish tank water, declining below detection levels after 15 days, suggesting an inhibitory effect of the autochthonous microflora of the water. When the aroA strain was used to inoculate sterilized water, its culturability was lower than that of wild-type strain A. hydrophila AG2; after long periods of incubation, aroA cells were able to enter a viable but nonculturable state. Entry into this nonculturable state was accompanied by changes in the cell morphology from rods to spheres, but the cells appeared to remain potentially viable, as assessed by the preservation of cell membrane integrity. Supplementation of the culture medium with sodium pyruvate favored the culturability and resuscitation of the two A. hydrophila strains at low temperatures (6 and 16°C). These results contribute to a better understanding of the behavior of the aroA strain in natural environments and suggest that the inactivation of the aroA gene may be beneficial for the safety of this live vaccine for aquacultures.

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Section: Discussionsupporting

confidence: 59%

Section: Cfu MLmentioning

confidence: 99%

Behavior of an Aeromonas hydrophila aroA Live Vaccine in Water Microcosms

Vivas¹,

Carracedo²,

Riaño³

et al. 2004

Appl Environ Microbiol

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“…VBNC forms appeared during the first days of the microcosm incubation ( figure 1a). Similar results were reported by LAABErKi (1999), LAKhAL (2003) and MAALEj et al (2004) for the survival of A. hydrophila in marine water, and for BrANDi et al (1999), MAry et al (2002) and MESSi et al (2002) for survival ability of A. hydrophila in different water types. The results obtained for A. hydrophila survival in marine water after incubation in waste water showed a greater progressive decline in the plate count number (2.74 log units) (figure 1b).…”

Section: Resultssupporting

confidence: 82%

Effect of Previous Incubation of Aeromonas hydrophila in Wastewater Prior to Its Transfer Into Marine Water Microcosms

Mejri

Bour

Boukef

et al. 2008

rseau

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AbstractThe occurrence of the mesophilic motile Gram negative non enterobacterial speciesA. hydrophilain the wild is a major problem that deserves to be resolved since it is a potentially pathogen able to enter into a non-culturable state on routine bacteriological plating media. These non-culturable forms can be detected by several direct or indirect visualization methods. This species has frequently been isolated from pathological forms in fish farming marine areas, especially near wastewater discharges. Consequently, we studiedA. hydrophilain marine water microcosms placed during a 24 hour period in treated waste waters and compared with the homologous strain not placed in the same conditions. Thus, two kinds of microcosms were prepared using filtered and autoclaved marine water or waste water, inoculated byA. hydrophilaand maintained at 25°C in darkness. The results obtained indicated thatA. hydrophilapopulation incubated at 25°C in marine water declined rapidly (3.21 log units in plate count number) during the first three days. Additionally, we noted thatA. hydrophilaincubated in marine water after a previous treatment in waste water declined progressively to 2.74 log units (in plate count number). Nevertheless, we showed no significant variations of the number of total bacterial cells forA. hydrophiladeveloped in marine water after prior treatment in waste water, despite the appearance of the VBNC form. During this state, rods of normal size, elongated cells and cocci were obtained. Concomitantly, we determined several changes in biochemical and antimicrobial patterns of stressedA. hydrophila, notably the acquisition of adipate metabolization and an increase of resistance to antimicrobial compounds, especially forA. hydrophilaincubated in marine water after treatment in waste water.

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“…Survival of Aeromonas in water microcosms is the subject of much debate as high-survival rates (Warburton et al 1994;Tsai and Yu 1997;Brandi et al 1999) and lowsurvival rates (Lowcock and Edwards 1994;Kersters et al 1996) have been reported. However, it is likely that interpretation of water viability studies depends, to a large extent, upon the specific conditions during and even before the actual experiments, including the bacterial strains used and their growth history (Rozen and Belkin 2001).…”

Section: Discussionmentioning

confidence: 99%

The effects of temperature, water activity and pH on the growth of Aeromonas hydrophila and on its subsequent survival in microcosm water

et al. 2003

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Aims: The influence of temperature, water activity and pH on the growth of Aeromonas hydrophila, and on its survival after transfer in nutrient-poor water were assessed. Methods and Results: Experiments were carried out according to a Box-Behnken matrix at 10-30°C, 0AE95-0AE99 water activity (a w ) and pH 5-9. The effect of each factor on the kinetic parameters of growth (i.e. the maximal specific growth rate, l max , and the lag time, k) and on the decline of the bacteria in microcosm water (time to obtain a reduction of 5 log, T 5 log ) were studied by applying central composite design. Conclusions: The major effect of temperature and water activity on the growth of A. hydrophila was highlighted, whereas the effect of pH in these experimental conditions was not significant. Models describing the effect of environmental parameters on the growth of A. hydrophila were proposed. The effect of the growth environment, and particularly the incubation temperature, have an influence on the survival ability of the bacteria in nutrient-poor water. Significance and Impact of the Study: The Box-Behnken design was well suited to determine the influence of environmental factors on the growth of A. hydrophila and to investigate the effect of previous growth conditions on its survival in microcosm water.

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Survival ability of cytotoxic strains of motile Aeromonas spp. in different types of water

Cited by 48 publications

References 18 publications

Behavior of an Aeromonas hydrophila aroA Live Vaccine in Water Microcosms

Behavior of an Aeromonas hydrophila aroA Live Vaccine in Water Microcosms

Effect of Previous Incubation of Aeromonas hydrophila in Wastewater Prior to Its Transfer Into Marine Water Microcosms

The effects of temperature, water activity and pH on the growth of Aeromonas hydrophila and on its subsequent survival in microcosm water

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