Survival, Adhesion and Migration of Metastatic Breast Tumor Cells: Roles of Parvins (PARVA and PARVB)

Abstract

Changes in cell-ECM interaction can lead to the anchorage-independent proliferation, resistance to apoptosis, and improved motility that characterize breast metastatic cells. The parvin, alpha (Parva) and parvin, beta (Parvb) localizes to focal adhesions and play a part in cell attachment, migration, and persistence. The assumption of our work is that parvins contribute to the capacity of tumor cells to spread. Following the extraction of primary (4T1T) and metastatic (4T1B and 4T1L) breast tumor cells from mouse metastatic breast cancer model, MTT assay and scratch test were utilized to determine the vitality and motility of the cells. qPCR was performed to examine the expression of parvins. Tumor cells did not show any difference in cellular viability in 2- or 3-dimensional cell culture. Our research illustrated that metastatic tumor cells have a higher capacity of migration. Molecular analysis revealed that parvins expression was significantly increased in metastatic breast carcinoma cells. These findings revealed new information about a major change in parvins expression in metastatic breast carcinoma cells. A targeted therapeutic approach against breast cancer metastasis could be developed using examination of the genetic characteristics of metastatic tumor cells.