Survival and distribution of Escherichia coli on diverse fresh-cut baby leafy greens under preharvest through postharvest conditions

Tomás‐Callejas, Alejandro; López-Velasco, Gabriela; Camacho, Alex B.; Artés, Francisco; Artés–Hernández, Francisco; Suslow, Trevor

doi:10.1016/j.ijfoodmicro.2011.08.027

Cited by 87 publications

(61 citation statements)

References 34 publications

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“…1 Table 3). This survival profile is not uncommon for both phyllosphere-competent isolates (Lindow & Brandl, 2003) and surrogates of enteric pathogens introduced to the phyllosphere by inoculation of lab-grown (Delaquis, Bach, & Dinu, 2007;GutierrezRodriguez et al, 2012;Moyne et al, 2011;Tomas-Callejas et al, 2011;Wood, Bezanson, Gordon, & Jamieson, 2010). Moyne et al (2011) using the same attenuated strain of E. coli O157:H7 as was used in this study, were able to recover the applied microorganism from lettuce surfaces by sample enrichment only after 2 days of inoculation with log 5 CFU/mL of a broth inoculum culture and up to 14 days post-inoculation.…”

Section: Discussionmentioning

confidence: 99%

Factors affecting cell population density during enrichment and subsequent molecular detection of Salmonella enterica and Escherichia coli O157:H7 on lettuce contaminated during field production

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Factors affecting cell population density during enrichment and subsequent molecular detection of Salmonella enterica and Escherichia coli O157:H7 on lettuce contaminated during field production

et al. 2015

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“…Changes in O 2 and CO 2 partial pressures within the baskets were monitored daily throughout storage, by withdrawing a 0.5 ml sample of the headspace from the baskets with a gas-tight syringe and determining O 2 and CO 2 levels by gas chromatography (Tomas-Callejas et al 2011). …”

Section: Post-harvest Product Management and Analysismentioning

confidence: 99%

Nutrient solution aeration and growing cycles affect quality and yield of fresh-cut baby leaf red lettuce

Conesa

Hernández

Niñirola

et al. 2015

AFSci

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The objective of this research was to study the effects of nutrient solution aeration (no aeration [NA], low aeration [LA] or high aeration [HA]) and growing cycle (autumn, winter and summer) on the yield, quality, and shelf life of red lettuce as a fresh-cut product grown in a floating system. The specific leaf area, yield and root diameter were affected by the growing cycle. The percentage of dry matter and the nitrate content were affected by growing cycle and aeration, total phenolics and mesophilic microorganism by aeration and storage time, hue angle and chromacity by growing cycle and storage time, and antioxidant capacity, vitamin C, lightness and psychrophilic microorganisms were affected by all three factors. NA conditions increased the antioxidant capacity in summer and vitamin C content in winter. The lowest mesophilic and psychrophilic count was observed in autumn. The effect of aeration on most of the quality parameters measured was influenced by the growing cycle.

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“…While the occurrence of the pathogen in leafy greens on that same sample date was relatively low, subsequent dissemination of high levels of E. coli O157:H7 from the feedlot surface to the plots may have increased the contamination of leafy greens that were sampled on the later dates. Numerous studies have evaluated the risks associated with preharvest contamination of leafy vegetables and have found that E. coli O157:H7 can persist on spinach and lettuce for days up to weeks (31)(32)(33)(34)(35)(36)(37)(38). Both growth chamber and field studies indicate that after inoculation onto leafy greens to mimic a contamination event, populations of E. coli O157:H7 initially decrease rapidly, followed by a more gradual rate of decline of remaining cells.…”

Section: Resultsmentioning

confidence: 99%

Effect of Proximity to a Cattle Feedlot on Escherichia coli O157:H7 Contamination of Leafy Greens and Evaluation of the Potential for Airborne Transmission

Berry

Wells

Bono

et al. 2015

Appl Environ Microbiol

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cThe impact of proximity to a beef cattle feedlot on Escherichia coli O157:H7 contamination of leafy greens was examined. In each of 2 years, leafy greens were planted in nine plots located 60, 120, and 180 m from a cattle feedlot (3 plots at each distance). Leafy greens (270) and feedlot manure samples (100) were collected six different times from June to September in each year. Both E. coli O157:H7 and total E. coli bacteria were recovered from leafy greens at all plot distances. E. coli O157:H7 was recovered from 3.5% of leafy green samples per plot at 60 m, which was higher (P < 0.05) than the 1.8% of positive samples per plot at 180 m, indicating a decrease in contamination as distance from the feedlot was increased. Although E. coli O157:H7 was not recovered from air samples at any distance, total E. coli was recovered from air samples at the feedlot edge and all plot distances, indicating that airborne transport of the pathogen can occur. Results suggest that risk for airborne transport of E. coli O157:H7 from cattle production is increased when cattle pen surfaces are very dry and when this situation is combined with cattle management or cattle behaviors that generate airborne dust. Current leafy green field distance guidelines of 120 m (400 feet) may not be adequate to limit the transmission of E. coli O157:H7 to produce crops planted near concentrated animal feeding operations. Additional research is needed to determine safe set-back distances between cattle feedlots and crop production that will reduce fresh produce contamination.T he consumption of fresh produce increasingly has been linked to human food-borne disease (1-4). In particular, leafy vegetables, such as spinach and lettuce, have become significant vehicles for the transmission of food-borne pathogens. Produce-associated outbreak surveillance data from the Centers for Disease Control and Prevention (CDC) for the period from 2000 to 2009 showed that among produce commodities, leafy greens were the most frequently linked to outbreaks (5). An analysis of published reports of U.S. fresh produce-associated outbreaks with an attribution risk ranking tool categorized the combination of leafy greens and Escherichia coli O157:H7 with the highest risk ranking score, followed by Salmonella enterica in tomatoes ranking second and S. enterica in leafy greens ranking third (6). For all U.S. foodborne disease outbreaks caused by Shiga toxin-producing E. coli (STEC) in the period of 1998 to 2008, leafy vegetables were the second most common implicated commodity, following beef (2). In addition, spinach, lettuce, and cilantro were commodities most associated with the recovery of STEC in produce market basket surveys conducted within the USDA Agricultural Marketing Service Microbiological Data Program from 2002 to 2012 (7). Leafy greens typically are consumed raw, so protecting these products from microbial contamination in the preharvest environment is critical to reducing the risk for food-borne illness. Potential sources of pathogens for preharvest produ...

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Survival and distribution of Escherichia coli on diverse fresh-cut baby leafy greens under preharvest through postharvest conditions

Cited by 87 publications

References 34 publications

Factors affecting cell population density during enrichment and subsequent molecular detection of Salmonella enterica and Escherichia coli O157:H7 on lettuce contaminated during field production

Factors affecting cell population density during enrichment and subsequent molecular detection of Salmonella enterica and Escherichia coli O157:H7 on lettuce contaminated during field production

Nutrient solution aeration and growing cycles affect quality and yield of fresh-cut baby leaf red lettuce

Effect of Proximity to a Cattle Feedlot on Escherichia coli O157:H7 Contamination of Leafy Greens and Evaluation of the Potential for Airborne Transmission

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