Survival and growth characteristics of bacteria in irradiated meat

Abstract

6.2 Introduction 15 8 6.3 Materials and Methods 160 6.3.1 Meat samples 6.3.2 Microbial recovery studies 6.3.3 Microbiological analyses 6.3.4 FCM analyses of spoiling pork patties 6.3.5 FCM analysis of retail samples 6.3.6 Quantification of bacterial cells using FCM 6.3.7 Evaluation of column materials 6.3.8 Clarification of meat suspensions for column material evaluation 6.3.8 Clarification of meat suspensions for flow cytometry 6.3.9 Flow cytometry 165 6.3.10 Staining protocol 165 6.3.12 Statistics 165 6.4 Results 165 6.4.1 Evaluation of column materials 165 6.4.2 Quantification of bacterial cells in meat homogenate using FCM 167

The ecology of Listeria monocytogenes in ready-to-eat (RTE) meats

“…Other methods of detecting VBNC cells include flow cytometry with fluorescent probes, fluorescent in situ hydridization (FISH) with 16S rRNA as the target, radiolabelling, and CTC-DAPI double staining to detect active electron transport chain (19,233,283,294).…”