Survival and growth of human preantral follicles after cryopreservation of ovarian tissue, follicle isolation and short-term xenografting

Paulini, Fernanda; Vilela, Janice M.V.; Chiti, Maria Costanza; Donnez, Jacques; Jadoul, Pascale; Dolmans, Marie-Madeleine; Amorim, Christiani Andrade

doi:10.1016/j.rbmo.2016.05.003

Cited by 91 publications

(81 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although knowledge of ovarian function and follicle population is necessary for implementation of reliable contraceptive methods to control fertility, ovarian reserve of preantral follicles and follicular density have not, to our knowledge, been studied in white-tailed deer. Conversely, studies on preantral follicle population have been conducted for several species, including: laboratory animals (rodents: [11] ; rabbits: [12] ), livestock (sheep: [13] ; goats: [14] ; horse: [15] ; cattle: [16] ), wildlife (macaques: [17] ; elephants: [18] ), and humans [19, 20] . Furthermore, studies have shown that ovarian reserve reduces as the female ages [21–23] .…”

Section: Introductionmentioning

confidence: 99%

Ovarian features in white-tailed deer (Odocoileus virginianus) fawns and does

et al. 2017

View full text Add to dashboard Cite

The knowledge about ovarian reserve is essential to determine the reproductive potential and to improve the methods of fertility control for overpopulated species, such as white-tailed deer (Odocoileus virginianus). The goal of this study was to evaluate the effect of age on the female reproductive tract of white-tailed deer, focusing on ovarian features. Genital tracts from 8 prepubertal and 10 pubertal females were used to characterize the preantral follicle population and density, morphology, distribution of follicular classes; stromal cell density; and apoptosis in the ovary. In addition, uterus and ovary weights and dimensions were recorded; and the number and the size of antral follicles and corpus luteum in the ovary were quantified. Overall, fawns had a greater (P < 0.05) preantral follicle population, percentage of normal follicles, and preantral follicle density than does. The mean stromal cell density in ovaries of fawns and does differed among animals but not between age groups. The apoptotic signaling did not differ (P > 0.05) between the ovaries of fawns and does. However, apoptotic ovarian cells negatively (P < 0.001) affected the preantral follicle morphology and density, and conversely, a positive correlation was observed with stromal cell density. As expected, the uteri and ovaries were larger (P < 0.002) and heavier (P < 0.001) in does than in fawns. In conclusion, this study has shown, for the first time, the preantral follicle population and distribution of classes, rate of morphologically normal follicles, and density of preantral follicles and stromal cells in white- tailed deer. Therefore, the findings herein described lead to a better understanding of the white-tailed deer ovarian biology, facilitating the development of new methods of fertility control.

show abstract

Section: Introductionmentioning

confidence: 99%

Ovarian features in white-tailed deer (Odocoileus virginianus) fawns and does

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Previous dissociation methods have been described in the literature: they’ve been used to isolate follicles from ovarian cortex [29–32] or discarded medulla tissue from women undergoing fertility preservation [33, 34]. The current study does not aspire to isolate ovarian follicles, but it aims to get an analyzable ovarian cell suspension for subsequent analysis by MFC.…”

Section: Discussionmentioning

confidence: 99%

Validation of an automated technique for ovarian cortex dissociation: isolation of viable ovarian cells and their qualification by multicolor flow cytometry

et al. 2017

View full text Add to dashboard Cite

BackgroundOvarian tissue cryopreservation is a technique for fertility preservation addressed to prepubertal girls or to patients for whom no ovarian stimulation is possible before initiation of gonadotoxic treatments. Autotransplantation of frozen-thawed ovarian tissue is the only available option for reuse but presents some limitations: ischemic tissue damages post-transplant and reintroduction of malignant cells in cases of cancer. It is therefore essential to qualify ovarian tissue before autograft on a functional and oncological point of view. Here, we aimed to isolate viable cells from human ovarian cortex in order to obtain an ovarian cell suspension analyzable by multicolor flow cytometry.MethodsOvarian tissue (fresh or frozen-thawed), from patients with polycystic ovarian syndrome (reference tissue) and from patients who underwent ovarian tissue cryopreservation, was used for dissociation with an automated device. Ovarian tissue-dissociated cells were analyzed by multicolor flow cytometry; the cell dissociation yield and viability were assessed. Two automated dissociation protocols (named laboratory and commercial protocols) were compared.ResultsThe effectiveness of the dissociation was not significantly different between reference ovarian tissue (1.58 × 106 ± 0.94 × 106 viable ovarian cells per 100 mg of ovarian cortex, n = 60) and tissue from ovarian tissue cryopreservation (1.70 × 106 ± 1.35 × 106 viable ovarian cells, n = 18). However, the viability was slightly different for fresh ovarian cortex compared to frozen-thawed ovarian cortex whether we used reference tissue (p = 0.022) or tissue from ovarian cryopreservation (p = 0.018). Comparing laboratory and commercial protocols, it appeared that cell yield was similar but cell viability was significantly improved when using the commercial protocol (81.3% ± 12.3% vs 23.9% ± 12.5%).ConclusionBoth dissociation protocols allow us to isolate more than one million viable cells per 100 mg of ovarian cortex, but the viability is higher when using the commercial dissociation kit. Ovarian cortex dissociation is a promising tool for human ovarian cell qualification and for ovarian residual disease detection by multicolor flow cytometry.

show abstract

“…A small volume of ovarian cortical tissue is sufficient for cryopreservation because most follicles reside in ovarian cortex rather than the medulla. However, it is reasonable to remove the entire ovary prior to gonadotoxic cancer treatment because it provides a sufficiently large pool of follicles even after follicular loss during the processing (Dolmans et al, ; J. Kim, Perez et al, ; Paulini et al, ; Smith et al, ). Therefore, if patients have high risks of losing fertility, cryopreserving one entire ovary can result in more satisfactory results.…”

Section: Issues Of Preserving Ovarian Tissuementioning

confidence: 99%

“…A superior efficacy of liberase, a mixture of purified enzymes, was reported in regard to follicular isolation outcomes (Dolmans et al, ). Many steps of follicular washing during the isolation procedure have been included to decrease the risk of malignant cell inclusion (Paulini et al, ). However, the retrieval rate of viable follicles with intact morphology and complete granulosa cell (GC) layers is still not satisfactory.…”

Section: Follicular Isolation and Selectionmentioning

confidence: 99%

“…Many studies reported transplanted follicular development in vivo and changes in follicular stage composition after grafting; however, the overall follicle recovery rate after transplantation has not fully been evaluated. A few studies reported the follicular recovery rates, which are useful in comparing the overall efficiencies of different candidate biomaterials (Paulini et al, ). They may help determine the number and distribution pattern of follicles in the delivery‐vehicle biomaterials.…”

Section: Designing Scaffolds For Isolated Follicle Deliverymentioning

confidence: 99%

See 1 more Smart Citation

A new possibility in fertility preservation: The artificial ovary

Cho

Kim

Noh

et al. 2019

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Conventional fertility preservation methods such as oocyte or embryo cryopreservation are currently insufficient to treat including those patients with prepubertal cancer and premature ovarian failure. Ovarian tissue cryopreservation presents as an alternative but has limitations with a potential risk of reintroducing malignant cells in patients who recover from cancer, those of chemotherapy prior to tissue cryopreservation. The so called “artificial ovary” aims to resolve this issue by transplanting isolated follicles with or without a biological scaffold. The artificial ovary may also offer an effective alternative option for those who cannot benefit from traditional assisted reproductive techniques such as in vitro fertilisation. To date, in animal studies and human trial, the artificial ovary restored endocrine function, achieved in vivo follicular development, and resulted in successful pregnancies. However, development of a technique for higher follicular recovery rate and a more optimised design of delivery scaffold, better transplantation techniques to prevent postsurgical ischemia, and consideration for genetic safety are required for safer and consistent human clinical applications. Ideas from different transplantation surgeries (e.g., entire ovary, ovarian cortex, and transplantation with tissue‐engineered products) can be applied to enhance the efficacy of artificial ovarian transplantation. For the better application of artificial ovary, a deeper understanding of mechanical and biochemical properties of the ovary and folliculogenesis after cryopreservation, transplantation with or without scaffold, and development of sophisticated in vivo imaging techniques of transplanted artificial ovary need to precede its efficient clinical application.

show abstract

Survival and growth of human preantral follicles after cryopreservation of ovarian tissue, follicle isolation and short-term xenografting

Cited by 91 publications

References 33 publications

Ovarian features in white-tailed deer (Odocoileus virginianus) fawns and does

Ovarian features in white-tailed deer (Odocoileus virginianus) fawns and does

Validation of an automated technique for ovarian cortex dissociation: isolation of viable ovarian cells and their qualification by multicolor flow cytometry

A new possibility in fertility preservation: The artificial ovary

Contact Info

Product

Resources

About