Survival and growth of triploid eastern oysters, Crassostrea virginica, produced from wild diploids collected from low-salinity areas

Bodenstein, Sarah; Callam, Brian R.; Walton, William C.; Rikard, F. Scott; Tiersch, Terrence R.; Peyre, Jérôme F. La

doi:10.1016/j.aquaculture.2022.739032

Cited by 27 publications

(17 citation statements)

References 61 publications

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“…The presence of multiple stressors preceding mortality events is consistent with both Guévélou et al (2019) and Matt et al (2020), who have noted that C. virginica triploid mortalities observed within Chesapeake Bay, VA are not correlated with the presence of elevated seawater temperature alone. Instead, common farm stressors, including tumbling stress and desiccation, as well as hyposalinity have been shown to contribute to triploid mortality (Bodenstein, 2019; Bodenstein et al, 2021, 2023). These results are consistent with the findings of this study that suggest that the synergistic impact of multiple environmental stressors can result in ploidy-specific differences in energy balance that contribute to differential survival.…”

Section: Discussionmentioning

confidence: 99%

“…However, field assessments of triploid and diploid oyster survivorship over the course of a growing cycle suggest that summer mortality is more complicated than would be expected from differences in thermal tolerance limits alone. Triploid C. gigas, C. virginica, and C. hongkongensis have been shown to exhibit lower (Gagnaire et al, 2006;Qin et al, 2019), higher (Bodenstein et al, 2021(Bodenstein et al, , 2023Guévélou et al, 2019;Houssin et al, 2019;Matt, 2018;Matt et al, 2020;Wadsworth, 2018;Wadsworth et al, 2019), and comparable (Dégremont et al, 2012;Ibarra et al, 2017) mortality rates when compared with diploids depending on the environmental conditions present. For example, Wadsworth et al (2019) found higher cumulative mortality in triploid C. virginica than diploids across growing sites within the Gulf of Mexico; mortalities were associated with sudden drops in salinity (< 5 ppt) and high temperature (> 30°C).…”

Section: Triploid Oysters Display Higher Mortality Rates Within Multi...mentioning

confidence: 99%

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Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following marine heatwaves

George

Cattau

Middleton

et al. 2023

Preprint

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Polyploidy has been shown to negatively impact environmental stress tolerance, resulting in increased susceptibility to extreme climate events such as marine heatwaves (MHWs). In this study, we used the response of the Pacific oysterCrassostrea gigasto MHWs as a model system to identify key ploidy-specific differences in the physiological and transcriptomic response of oysters to environmental stress. In this study, adult diploid (2n) and triploid (3n) oysters were exposed to elevated seawater temperature (single stressor; 30°C), elevated temperature followed by acute desiccation stress (multiple stressor; 30°C + emersion at an aerial temperature of 44°C for 4h), or a control (17°C) within a hatchery setting. Oyster mortality rate was elevated within stress treatments with respect to the control and was significantly higher in triploids than diploids following multiple stress exposure (36.4% vs. 14.8%). Triploids within the multiple stressor treatment exhibited signs of energetic limitation, including metabolic depression, a significant reduction in ctenidium Na+/K+ATPase activity, and the dysregulated expression of key genes associated with heat tolerance, the inhibition of apoptosis, and mitochondrial function. Functional enrichment analysis of ploidy-specific gene sets identified that biological processes associated with metabolism, stress tolerance, and immune function were overrepresented within triploids across stress treatments. Our results demonstrate that triploidy impacts the transcriptional regulation of key metabolic processes that underly the environmental stress response of Pacific oysters, resulting in downstream shifts in physiological tolerance limits that may be detrimental to survival. The impact of chromosome set manipulation on the climate resilience of marine populations has important implications for the adaptability of marine populations and domestic food security within future climate scenarios, especially as triploidy induction becomes an increasingly popular tool to elicit reproductive control across a wide range of marine organisms used within marine aquaculture.

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Section: Discussionmentioning

confidence: 99%

Section: Triploid Oysters Display Higher Mortality Rates Within Multi...mentioning

confidence: 99%

Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following marine heatwaves

George

Cattau

Middleton

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Triploid C. gigas , C. virginica , and C. hongkongensis have been shown to exhibit lower (Gagnaire et al, 2006; Qin et al, 2019), higher (Bodenstein et al, 2021, 2023; Guévélou et al, 2019; Houssin et al, 2019; Matt, 2018; Matt et al, 2020; Wadsworth, 2018; Wadsworth et al, 2019), and comparable (Dégremont et al, 2012; Ibarra et al, 2017) mortality rates when compared with diploids depending on the environmental conditions present. For example Wadsworth et al (2019) found higher cumulative mortality in triploid C. virginica than diploids across growing sites within the Gulf of Mexico; mortalities were associated with sudden drops in salinity (<5 ppt) and high temperature (>30°C).…”

Section: Discussionmentioning

confidence: 99%

“…The presence of multiple stressors preceding mortality events is consistent with both Guévélou et al (2019) and Matt et al (2020), who have noted that C. virginica triploid mortalities within Chesapeake Bay, VA are not correlated with the presence of elevated seawater temperature alone. Instead, common farm stressors, including tumbling stress and desiccation, as well as hyposalinity have been shown to contribute to triploid mortality (Bodenstein, 2019; Bodenstein et al, 2021, 2023). These results are consistent with the findings of this study that suggest that the synergistic impact of multiple environmental stressors motivates triploid mortality.…”

Section: Discussionmentioning

confidence: 99%

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Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves

George

Cattau

Middleton

et al. 2023

Global Change Biology

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Polyploidy has been suggested to negatively impact environmental stress tolerance, resulting in increased susceptibility to extreme climate events. In this study, we compared the genomic and physiological response of diploid (2n) and triploid (3n) Pacific oysters (Crassostrea gigas) to conditions present during an atmospheric heatwave that impacted the Pacific Northwestern region of the United States in the summer of 2021. Climate stressors were applied either singly (single stressor; elevated seawater temperature, 30°C) or in succession (multiple stressor; elevated seawater temperature followed by aerial emersion at 44°C), replicating conditions present within the intertidal over a tidal cycle during the event. Oyster mortality rate was elevated within stress treatments with respect to the control and was significantly higher in triploids than diploids following multiple stress exposure (36.4% vs. 14.8%). Triploids within the multiple stressor treatment exhibited signs of energetic limitation, including metabolic depression, a significant reduction in ctenidium Na+/K+ ATPase activity, and the dysregulated expression of genes associated with stress response, innate immunity, glucose metabolism, and mitochondrial function. Functional enrichment analysis of ploidy‐specific gene sets identified that biological processes associated with metabolism, stress tolerance, and immune function were overrepresented within triploids across stress treatments. Our results suggest that triploidy impacts the transcriptional regulation of key processes that underly the stress response of Pacific oysters, resulting in downstream shifts in physiological tolerance limits that may increase susceptibility to extreme climate events that present multiple environmental stressors. The impact of chromosome set manipulation on the climate resilience of marine organisms has important implications for domestic food security within future climate scenarios, especially as triploidy induction becomes an increasingly popular tool to elicit reproductive control across a wide range of species used within marine aquaculture.

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In-situ valve opening response of eastern oysters to estuarine conditions

Lavaud,

Archer,

La Peyre

et al. 2024

Mar Biol

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High-frequency recordings of valve opening behavior (VOB) in bivalves are often used to detect changes in environmental conditions. However, generally a single variable such as temperature or the presence of toxicants in the water is the focus. A description of routine VOB under non-stressful conditions is also important for interpreting responses to environmental changes. Here we present the first detailed quantitative investigation of the in-situ VOB of eastern oysters (Crassostrea virginica) to environmental variables typically not considered stressful. The VOB of eight individuals was monitored for seven weeks in a Louisiana estuary. We examined the relationships between VOB metrics (variance in mean % max opening among oysters, the probability of an oyster being closed, and the rate of valve closure), and temperature, salinity, chlorophyll-a (chl-a) concentration, the rate of change in those environmental variables, and the rate of change in water depth. Relationships were analyzed through statistical models including rates of change over 0, 0.25, 1-, 6-, 12-, and 24-hours. All the responses were best explained by the 12-hour time step model. The interaction effect between salinity and the rate of change of salinity had the greatest impact on variance in oysters’ behavior. Oysters closed faster at higher salinities and were more likely to be closed at lower chl-a concentrations. Significant interactions were found between many environmental variables, indicating a high level of complexity of oyster behavior in the natural environment. This study contributes to a better understanding of the impact of environmental conditions on oyster behavior and can help inform predictive tools for restoration initiatives and fisheries practices.

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Survival and growth of triploid eastern oysters, Crassostrea virginica, produced from wild diploids collected from low-salinity areas

Cited by 27 publications

References 61 publications

Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following marine heatwaves

Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following marine heatwaves

Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following heatwaves

In-situ valve opening response of eastern oysters to estuarine conditions

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