Survival and Immunity of Marron Cherax cainii (Austin, 2002) Fed Bacillus mycoides Supplemented Diet under Simulated Transport

Abstract

The present study examined the effect of simulated transport on marron, Cherax cainii, (Austin, 2002) after a 10 week feeding trial using basal diet or customised probiotic, Bacillus mycoides supplemented diet by measuring intestinal bacterial population, total haemocyte count (THC), bacteraemia, morbidity, dehydration and mortality.Packing steps followed the established standard packing method for live transportation of marron. Each treatment group consisted of six polystyrene boxes (65 × 30 × 40 cm 3 ), and each box contained 30 marron from each feeding group. The sealed boxes were placed on a trolley at room temperature to give the effect of transportation. Boxes were opened at 24 th and 48 th hour post simulated transport and marron from each treatment group were returned to the culture tank. After temperature acclimation, the marron were observed for mortality and samples were collected to assess marron health and immunity.The results demonstrated that no mortality was observed at 24 h of transport both in basal diet and probiotic diet fed marron, however at 48h of transport the survival of probiotic fed marron was significantly higher (100 ± 0.0%) compared to survival (93.3 ± 2.8%) of basal diet fed marron. The higher survival rate of probiotic fed marron was also sustained by superior health and immune status indicated by higher intestinal bacterial population, higher total haemocyte count and lower haemolymph bacteria (bacteraemia) level. In brief, supplementation with host origin customized probiotic B. mycoides significantly improved marron tolerance to a live transport stress test, which resulted in no mortality up to 48h of transport.

Dietary Effects of Lactobacillus plantarum Combined with Galactooligosaccharide on Immunological and Biochemical Parameters, Gut Microbiota, Digestive Enzyme Activity, Body Composition, and Stress Resistance in Narrow-Clawed Crayfish, Pontastacus leptodactylus (Eschscholtz, 1823)

“…Similar to previous studies [3,5], during air exposure stress, no sign of mortality was detected in all GLP-supplemented and control groups. Consistent with the obtained results, studies conducted on marron (Cherax cainii) fed pre-or probiotic revealed that 24 h subjecting to air [59] as well as 36 h ( [1]) did not alter the survival rate. However, GLP-supplemented crayfish in the present study demonstrated better performance in response to stress due to enhance THC, SGC, HC, and CAT activities compared to the control.…”

“…Additionally in a study conducted on marron, different MOS inclusion levels could significantly enhance THC and LGC proportion compared to the unsupplemented group after air exposure ( [1]). Moreover, higher health status in marron fed Bacillus mycoides represented by higher THC levels following air exposure stress [59].…”

“…Air exposure stress is considered one of the most practical stressors applied at the end of the nutritional experiments to evaluate the health status of crustaceans [1,3,23,59]. In this regard, the hemolymph immunological indices such as THC and DHC ( [19,60,61]), PO [20,62], LYZ [5,21], SOD, and CAT [63][64][65], as well as biochemical parameters, e.g., glucose and TPP [17,18,66], have already been applied as sensitive biomarkers of air exposure stress effects in shellfish.…”

Dietary supplementation of black soldier fly (Hermetica illucens)meal modulates gut microbiota, innate immune response and health status of marron (Cherax cainii, Austin 2002) fed poultry-by-product and fishmeal based diets

“…Yet, the production has remained stagnant over the decade (Van Mai & Fotedar, 2018). To improve the production potential, several investigations have been conducted to find the suitable combination of feed for marron (Ambas, Suriawan & Fotedar, 2013; Ambas & Fotedar, 2015; Ambas, Buller & Fotedar, 2015). As an omnivorous animal, the protein requirement for marron is moderate (approximately 30%) (Ambas, Fotedar & Buller, 2017).…”