Survival and integration of transplanted postmitotic human neurons following experimental brain injury in immunocompetent rats

Abstract

Long-term survival and integration of NT2N cells in the periinjured cortex of immunocompetent rats provides the researcher with an important cellular system that can be used to study maturation, regulation, and neurite outgrowth of transplanted neurons following TBI.

Differential effects of spinal cord gray and white matter on process outgrowth from grafted human NTERA2 neurons (NT2N, hNT)

“…Based on the data presented here, we conclude that the NT2N neuronal model system can be exploited to develop novel strategies for investigating the neurobiology of transplanted human neurons and to develop new transplant therapies for patients with CNS diseases (Trojanowski et al, 1997;Borlongan et al, 1998;Phillips et al, 1999). Indeed, studies of grafted human NT2N neurons may clarify host molecular signals that support and direct the successful integration of grafted human neurons and their processes into diverse regions of the nervous system.…”

Migration, fate and in vivo imaging of adult stem cells in the CNS

“…In addition to information about grafted cells, MRI can provide information about the surrounding tissue (i.e., lesion size, edema or inflammation), which may have an effect on graft survival or the functional recovery of the tissue. [27][28][29][30] …”

Cell therapies for traumatic brain injury

“…65 Adult human neurons have been shown to survive up to 4 weeks posttransplantation into traumatically injured rat cortex, but no significant improvement in behavioral function has been identified. 49,51 More recently, these postmitotic human neural cells have been shown to survive for 12 weeks in immunosuppressed rats after delayed transplantation following TBI, although no improvement in behavioral function was reported. 79 Primed human NSCs transplanted into the hippocampus after fluid percussion injury in rats were found to differentiate to neurons, produce neurotropic factors, and mediate cognitive improvement.…”