Survival and metabolic activity of the GanedenBC³⁰ strain of Bacillus coagulans in a dynamic in vitro model of the stomach and small intestine

Abstract

We have investigated the survival and activity of GanedenBC(30) during passage through the upper gastro-intestinal tract. GanedenBC(30) was tested in a dynamic, validated, in vitro model of the stomach and small intestine (TIM-1) on survival and its potential to aid in digestion of milk protein, lactose and fructose. The survival of GanedenBC(30) was high (70%), although germination of the spores was minimal (<10%) under the conditions tested. Survival of the strain in the presence of lactose and fructose was markedly lower (56-59%) than in the absence of the sugars. The amount of digested milk protein available for absorption was somewhat higher (+0.2 g) when GanedenBC(30) was added to the milk. When GanedenBC(30) was tested with lactose or fructose added to the meal, the cumulative amount of lactate produced was slightly higher (+0.12-0.18 mmol) compared to the GanedenBC(30) alone. In conclusion, although the differences in survival of GanedenBC(30) are small, these results show the potential of GanedenBC(30) to aid in protein digestion and in the digestion of lactose and fructose. If a larger fraction of the Bacillus coagulans cells had germinated, the influence on protein and carbohydrate digestion would probably have been much greater. Importance of the findings: the potential of GanedenBC(30) to aid in the digestion of lactose and fructose could be used to prevent occurrence of intestinal symptoms in individuals sensitive to these carbohydrates.

Evaluation of the stability of Bacillus coagulans MTCC 5856 during processing and storage of functional foods

“…After every time interval for the stability studies, 1.0 g of sample (baked food or other studied product containing B. coagulans MTCC 5856) was thoroughly mixed in sterile saline (0.9% NaCl, w/v) and then incubated in water bath for 30 min at 75°C, followed by immediate cooling to below 45°C. This suspension was further serially diluted in sterile saline and the viable count was enumerated by plating on glucose yeast extract agar (HiMedia, Mumbai, India) by pour plate method (Maathuis et al, 2010). The plates were incubated at 37°C for 48-72 h. Each analysis was performed in triplicate at two different occasions.…”

“…Waffles were stored in freezer (À20°C AE 2) and the viable count of B. coagulans MTCC 5856 was enumerated by dissolving in sterile saline (0.9% NaCl, w/v). Samples were analysed at 0, 1, 2, 3, 6 and 12 months (ICH (International Conference on Harmonisation), 2003) by serial dilution method (Maathuis et al, 2010).…”

“…It was baked for 20-25 min at 205°C and then cooled completely and stored in the freezer (À20°C AE 2). The viable count of B. coagulans MTCC 5856 was enumerated by dissolving muffins in sterile saline (0.9% NaCl, w/v) followed by serial dilution method (Maathuis et al, 2010). Samples were analysed at 0, 1, 2, 3, 6 and 12 months (ICH (International Conference on Harmonisation), 2003).…”

“…Co.). Samples were withdrawn before and after brewing followed by holding the temperature at 77°C for 0.5, 1.0, 2.0 and 4.0 h. Samples were analysed for viable spore counts by serial dilution method (Maathuis et al, 2010).…”

Preparation of File (Sohan) by using the Probiotics with Chemical and Microbiologic Analysis for Individual's Health

“…Probiotics help to the treatment of kidney stones, preventing from constipation in the elderly, absorption and production of vitamin, digestion of lactose in the colon and reducing blood cholesterol and carcinogens and preventing from allergy etc. [4,5].…”