Survival and multiplication ofRhizobium japonicum strains in silt loam

Pugashetti, Balappa K.; Wagner, George H.

doi:10.1007/bf02205850

“…While strains IRC291, JRC29 and MI-50A attained the highest numbers (logs 10.0, 10.4 and 10.2 respectively), cell numbers of JRW3 reached log 9.1 during 17-24 days at 30°C (Fig. Similar results, such as an initial increase followed by a decline in numbers of R. japontcum in sterile soil, were also obtained by Pugashetti and Wagner [18]. Survival of strains JRC29 and JRW3 in bauxitic soil was significantly affected by temperature as both of the strains survived poorly at 37°C compared to at 30°C (Fig.…”

Section: Resultssupporting

confidence: 71%

Growth and survival of cowpea rhizobia in bauxitic silt loam and sandy clay loam soils

Aarons¹

1987

FEMS Microbiology Letters

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Survival of 4 cowpea Rhizobium strains, IRC291, MI‐50A, JRW3 and JRC29, in two soil types (bauxitic silt loam and sandy clay loam) undergoing drying at 30°C and 37°C was examined. While all strains except JRW3 showed a general pattern of increase in their numbers during the first 3 weeks in sterile soils, none of the strains showed any increase in their population in non‐sterile soils. Cowpea rhizobia showed better survival in non‐sterile bauxitic silt loam than in clay loam soils at 30°C. However, the long‐term survival (examined up to 6 months) of rhizobia in both soils was poor at 37°C as compared to 30°C. We also found that cowpea rhizobia survived better in soils undergoing drying than in moist soils at 30°C. Our results suggest that (a) cowpea rhizobia survived better in bauxitic silt loam than in clay loam soil and (b) the low indigenous cowpea rhizobial population in Jamaican soils may be due to their poor long‐term survival and weak saprophytic competence.

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“…2a, c). Similar results, such as an initial increase followed by a decline in numbers of R. japontcum in sterile soil, were also obtained by Pugashetti and Wagner [18]. When the effect of soil type (bauxitic silt loam and sandy clay loam) on the survival of cowpea rhizobia was examined in sterile soils, there was no significant difference (p = 0.05) in their survival at 30°C (Figs.…”

Section: Io-ilsdsupporting

confidence: 83%

Growth and survival of cowpea rhizobia in bauxitic silt loam and sandy clay loam soils

Aarons

¹

,

Ahmad

²

1987

FEMS Microbiology Letters

3

0

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Survival of 4 cowpea Rhizobium strains, IRC291, MI‐50A, JRW3 and JRC29, in two soil types (bauxitic silt loam and sandy clay loam) undergoing drying at 30°C and 37°C was examined. While all strains except JRW3 showed a general pattern of increase in their numbers during the first 3 weeks in sterile soils, none of the strains showed any increase in their population in non‐sterile soils. Cowpea rhizobia showed better survival in non‐sterile bauxitic silt loam than in clay loam soils at 30°C. However, the long‐term survival (examined up to 6 months) of rhizobia in both soils was poor at 37°C as compared to 30°C. We also found that cowpea rhizobia survived better in soils undergoing drying than in moist soils at 30°C. Our results suggest that (a) cowpea rhizobia survived better in bauxitic silt loam than in clay loam soil and (b) the low indigenous cowpea rhizobial population in Jamaican soils may be due to their poor long‐term survival and weak saprophytic competence.

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“…Pankhurst (25) showed that slowgrowing strains of Lotus rhizobia possessed higher levels of resistance to TET, RIF, and KAN than fast-growing strains. Working with three wild-type R. japonicum strains, Pugashetti and Wagner (27) found a high degree of sensitivity to KAN, STR, and TET and tolerance to 100 to 200 ,ug of RIF per ml. Wide variability of resistance to STR in peanut Rhizobium has.been reported (20).…”

Section: Discussionmentioning

confidence: 98%

“…The use of genetic markers as a means of strain identification was first demonstrated under laboratory conditions with mutants that were resistant to high levels of antibiotics (11,21,24,25,(27)(28)(29)(30) and auxotrophic mutants (15,24). Several workers have used antibiotic resistance successfully to identify rhizobia in inoculation studies conducted in the field (4,12,19,20).…”

mentioning

confidence: 99%

Nodulation Efficiency of Legume Inoculation as Determined by Intrinsic Antibiotic Resistance

Kremer

¹

,

Peterson

²

1982

Appl Environ Microbiol

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Patterns of intrinsic resistance and susceptibility to different levels of antibiotics were determined for strains of both fastand slow-growing rhizobia. These patterns were stable to plant passage when they were used to identify Rhizobium strains in nodule suspensions or nodule isolates. The method of identification by intrinsic resistance and susceptibility patterns was reliable for identifying strains in field nodules when strains were first isolated from the nodules to provide a standard inoculum size and then typed on antibiotic-containing media. Other patterns of resistance were encountered during identification of field isolates; these patterns may have resulted from acquired resistance to certain antibiotics or from mixed infections of the nodules. The occurrence of resistance patterns identical to those of inoculant strains among native strains was directly related to the size of the soil population. High strain recovery was associated directly with high rates of inoculation.

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Survival and multiplication ofRhizobium japonicum strains in silt loam

Cited by 8 publications

References 28 publications

Growth and survival of cowpea rhizobia in bauxitic silt loam and sandy clay loam soils

Growth and survival of cowpea rhizobia in bauxitic silt loam and sandy clay loam soils

Growth and survival of cowpea rhizobia in bauxitic silt loam and sandy clay loam soils

Nodulation Efficiency of Legume Inoculation as Determined by Intrinsic Antibiotic Resistance

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