Survival and recovery of viable but noncultivable forms of Campylobacter in aqueous microcosm

Talibart, Roland; Denis, M.; Castillo, Ana Ruiz; Cappelier, Jean‐Michel; Ermel, Gwennola

doi:10.1016/s0168-1605(00)00201-4

Cited by 53 publications

(36 citation statements)

References 10 publications

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“…It confirms the efficacy of the embryonated egg model as described in previous works in Campylobacter jejuni VBNC cells [10,13,47]. Moreover, two important findings described in this work need to be taken into account.…”

Section: Discussionsupporting

confidence: 90%

“…VBNC cells of various pathogens have also been recovered in animal models: mice for Vibrio vulnificus [35] and Campylobacter jejuni [10,24]; rat gut for Campylobacter jejuni [45]; chicks for Campylobacter jejuni [10,46]; rabbit ileal loop for Vibrio cholerae [15]; human volunteers for Vibrio cholerae [15] and fertilised eggs for Legionella pneumophilla [21] and Campylobacter jejuni [11,14]. For this species, it appears that inoculation of VBNC cells in the yolk sac of embryonated eggs was the best way to obtain recovery at a culturable state [11,14,47]. In contrast, Medema et al [31] did not succeed in recovering VBNC cells of Campylobacter jejuni after inoculation in fertilised eggs.…”

Section: Discussionmentioning

confidence: 99%

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Avirulent Viable But Non Culturable cells ofListeria monocytogenesneed the presence of an embryo to be recovered in egg yolk and regain virulence after recovery

et al. 2007

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-The aim of this study was to assess the efficiency of the embryonated egg model to recover Viable But Non Culturable (VBNC) cells of Listeria monocytogenes. L. monocytogenes cells were incubated in filtered sterilised distilled water. The VBNC state was obtained after a 25 to 47 days incubation period (concentration of culturable cells less than 1 cfu/mL). Fifteen days after the VBNC state was reached, non culturability was checked in various media. One milliliter of each VBNC suspension that contained 10 4 metabolically active cells (i.e. Direct Viable Count + cells) was inoculated into the vitellus fluid of embryonated and non-embryonated eggs. Culturable cells were detected in a large proportion of the embryonated eggs (18/32), but not in the non-embryonated eggs (1/32). The recovery rate was higher after culture of the vitellus fluid plus embryo (18/32) than after culture of the vitellus fluid alone (6/32). The results indicate that the embryo likely plays a prominent part in the recovery process. The virulence of recovered cells was assessed by the ability to form plaques in HT-29 cell monolayers and by the ability to colonise mouse spleens. Although the cells were classified as avirulent when in the VBNC state, the virulence was recovered after resuscitation.VBNC / virulence / embryonated eggs / Listeria monocytogenes

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Avirulent Viable But Non Culturable cells ofListeria monocytogenesneed the presence of an embryo to be recovered in egg yolk and regain virulence after recovery

et al. 2007

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“…After 48 days of storage, in water containing less than 1 CFU (Colony Forming Unit) per 2 ml as determined by the plate count technique, filtration of the whole bottle of mineral water and enrichment in Preston broth according to the NF-ISO 10272 norm were performed in order to enhance the detection threshold and detect remaining culturable cells (Talibart et al, 2000).…”

Section: Recovery In Embryonated Eggs Of C Jejuni Viable But Non Culmentioning

confidence: 99%

Survival of Campylobacter jejuni in mineral bottled water according to difference in mineral content: Application of the Weibull model

Guillou

Leguérinel²,

Garrec³

et al. 2008

Water Research

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The aim of the study was to examine the hypothesis emitted by Evans et al. (2003) that mineral bottled water accidentally contaminated by Campylobacter jejuni would represent a risk factor for Campylobacter infection.Culturability of C. jejuni cells inoculated in low and high mineral bottled water during storage at 4°C in the dark was performed by surface plating and modelled using the Weibull model.The loss of C. jejuni culturability observed in all conditions tested was shown to be dependent from strain, preculture condition and water composition.Following inoculation of C. jejuni, the rapid loss of culturability was not correlated to complete cell death as the passage into embryonated eggs enabled recovery of cells from the Viable But Non Culturable state.In conclusion, the sanitary risk associated to contaminated bottled water can not be excluded although it is presumably low. Culture conditions, strain and water type must be taken into account in the evaluation of the risk factor as they influence significantly Campylobacter survival in water.

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“…However, this method is time consuming and laborious, requiring prolonged incubation, selective enrichment, and reduction of the background flora, which should be followed by biochemical identification tests (12,13). Most laboratories are unable to diagnose the anaerobic, microaerophilic, and fastidious bacteria responsible for human gastrointestinal disorders using conventional microbiological methods.…”

Section: Introductionmentioning

confidence: 99%

“…Polymerase chain reaction (PCR) is a powerful technique for the detection of the target DNA in various clinical specimens, including fecal samples. However, fecal specimens often contain substances that may interfere with the PCR assay, leading to false-positive or false-negative results (2,4,11,13,15,22). Accordingly, its application in clinical laboratories needs validation.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Detection Limits of the Culture and PCR Methods for the Detection of Clostridium difficile, Clostridium perfringens, Campylobacter jejuni, and Yersinia enterocolitica in Human Stool

Ganji

Azimirad

Farzi

et al. 2016

Arch Pediatr Infect Dis

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Background: Detection of fastidious enteropathogenic bacteria in fecal samples of patients with gastroenteritis is a challenge in clinical microbiological laboratories. Objectives: The aim of this study was to compare the detection limits of the PCR and culture methods for the diagnosis of Campylobacter spp., Yersinia spp., Clostridium perfringens, and Clostridium difficile in human stool samples. Methods: Healthy human stool and sterile phosphate-buffered saline (PBS) samples were separately spiked with 10-fold dilutions of C. jejuni, C. difficile, Y. enterocolitica, and C. perfringens reference strains to obtain final concentrations of 10 1 -10 8 colony forming

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Survival and recovery of viable but noncultivable forms of Campylobacter in aqueous microcosm

Cited by 53 publications

References 10 publications

Avirulent Viable But Non Culturable cells ofListeria monocytogenesneed the presence of an embryo to be recovered in egg yolk and regain virulence after recovery

Avirulent Viable But Non Culturable cells ofListeria monocytogenesneed the presence of an embryo to be recovered in egg yolk and regain virulence after recovery

Survival of Campylobacter jejuni in mineral bottled water according to difference in mineral content: Application of the Weibull model

Comparison of the Detection Limits of the Culture and PCR Methods for the Detection of Clostridium difficile, Clostridium perfringens, Campylobacter jejuni, and Yersinia enterocolitica in Human Stool

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