Survival and Recovery of Yeast Cells After Simultaneous Treatment of UV Light Radiation and Heat¶

Kim, Jin Kyu; Петин, В.Г.; Tkhabisimova, Marianna D.

doi:10.1562/2003-11-21-ra.1

Cited by 9 publications

(9 citation statements)

References 21 publications

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“…Fig. 3 shows the averaged data of our previously published results (4,16,19) . The survival curves of diploid yeast cells (S. cerevisiae, strain XS800) subjected to simultaneous treatment with heat and UV radiation are presented at &igure 3A.…”

Section: Resultsmentioning

confidence: 85%

“…Whereas considerable knowledge about repair processes in UV-irradiated cells has been gained during past decades (21) , the situation is far less clear for the combined action of UV light with other inactivating factors. It seems widely accepted now that the synergistic interaction of the UV light combined with other agents is displayed by decreased repair rates at molecular and cellular levels (16,21) . It is not clear, however, whether or not the LHR inhibition may serve as an indicator of the signi&icant increase in cell sensitivity to UV light.…”

Section: Resultsmentioning

confidence: 99%

“…Curves were &itted to the data points by eye. Other details of materials and methods can be found in our previous papers (15)(16)(17) .…”

Section: Methodsmentioning

confidence: 99%

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Respiratory mutant and liquid holding recovery inhibition in yeast cells

Евстратова

Петин

Pronkevich

2017

IJRR

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Background: Cell ability to recover from radia on damage is of great relevance in cancer treatment. It is o en believed that the inhibi on of cell ability to the liquid holding recovery (LHR) may be an indicator of the overall suppression of cell ability to recover from poten ally lethal radia on damage. However, the literature contains no experimental evidence whether the LHR inhibi on may always serve as marker of the significant increase in cell sensi vity to damaging agents. Materials and Methods: In experiments described here the yeast cells were used as a model for eukaryo c cells. The dose-response curves and recovery kine cs were determined by colony assay a er simultaneous treatment of heat with ionizing or UV radia ons as well as a er the simultaneous ac on of ionizing radia on and cispla n. The cell survival was es mated by both microscopic method and colony forming ability. Results: It is demonstrated that the recovery may take place on nutrient media. The complete inhibi on of cell recovery a er simultaneous heat treatment with ionizing radia on or UV light is accompanied with the significant increase in cell sensi vity to these agents, the actual increase being more appreciable than that expected a er the inhibi on of the LHR only. This rule is not universal as it was demonstrated for diploid yeast cells exposed to ionizing radia on and cispla n. Conclusion: The LHR takes place on nutrient media during the delay of the first pos rradia on division. The LHR inhibi on may not always indicate the suppression of other dark recovery processes and the corresponding increase in cell radiosensi vity.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

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Respiratory mutant and liquid holding recovery inhibition in yeast cells

Евстратова

Петин

Pronkevich

2017

IJRR

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“…Error bars in all figures show interexperimental errors. Other details were as described previously (15,16,20,22). Figure 1 shows a typical example of the basic experimental data observed in this study for the recombinant frequency of yeast cells (S. cerevisiae, strain T1) as a function of UV dose or exposure time.…”

Section: Methodsmentioning

confidence: 94%

“…Error bars in all figures show interexperimental errors. Other details were as described previously (15,16,20,22).…”

Section: Methodsmentioning

confidence: 99%

The Fluence Rate Determines the Synergistic Interaction of UV Radiation and Heat for Mitotic Recombination and Cell Inactivation in Yeasts

Kim

Komarova

Zhurakovskaya

et al. 2006

Photochem & Photobiology

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The significance of the UV fluence rate for the synergistic interaction of UV light (254 nm) and heat was demonstrated for the frequency of mitotic recombination in a wild-type diploid yeast of Saccharomyces cerevisiae (strain T1) and for cell inactivation of two wild-type diploid yeast of S. cerevisiae (strains T1, XS800). It was shown for mitotic recombination that a decrease in the intensity of UV exposure results in the necessity of decreasing the temperature at which UV irradiation occurs to provide the same value of the synergistic enhancement ratio. For cell inactivation, there was a specific temperature maximizing the synergistic effect for any constant fluence rate and the temperature range, synergistically increasing the inactivation effect of UV radiation, should be shifted to lower temperatures with a decrease in the fluence rate. To interpret the results observed, a simple mathematical model of the synergistic interaction was applied. The model suggests that the synergistic interaction of UV light and hyperthermia is expected to result from some additional effective damages arising from the interaction of some sublesions induced by both agents. On the basis of data obtained, it was supposed that the synergistic interaction of these factors might take place at small intensities of UV light and temperatures existing in the biosphere. In other words, for a long duration of interaction, which is important for problems of UV light protection and health effects, one can expect a synergistic interaction of this factor with environmental heat or physiological temperatures and thereby an increase in their inactivating and genetic consequences.

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