Survival and transmissibility of Pasteurella                pneumotropica

Scharmann, W.; Heller, Allen H.

doi:10.1258/0023677011911543

Cited by 36 publications

(24 citation statements)

References 7 publications

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“…On gram stain (Figure 6b), the pulp chamber contained both gram-positive cocci and gram-negative coccobacilli that were identified as Streptococcus mitus and Pasteurella pneumotropica , respectively, bacteria common in oral flora of humans and rodents. 28,29 There was severe periodontal disease characterized by periapical abscesses; inflammation extended through the apical foramen into the periradicular tissue, causing destruction of roots and alveolar bone (Figure 6c). TRAP stains confirmed extensive osteoclast-mediated bone loss around the roots (Figure 6d).…”

Section: Resultsmentioning

confidence: 99%

Hyperglycemia and xerostomia are key determinants of tooth decay in type 1 diabetic mice

Yeh

Harris

Mohan

et al. 2012

Laboratory Investigation

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Insulin-dependent type 1 diabetes mellitus (DM) and oral diseases are closely interrelated. Poor metabolic control in diabetics is associated with a high risk of gingivitis, periodontitis and tooth loss. Salivary flow declines in diabetics and patients suffer from xerostomia. Reduced saliva predisposes to enamel hypomineralization and caries formation; however, the mechanisms that initiate and lead to progression of tooth decay and periodontitis in type 1 DM have not been explored. To address this issue, we analyzed tooth morphology in Akita −/− mice that harbor a point mutation in the Ins2 insulin gene, which leads to progressive hyperglycemia. Mandibles from Akita −/− and wild-type littermates were analyzed by microCT, scanning EM and histology; teeth were examined for amelogenin (Amel) and ameloblastin (Ambn) expression. Mice were injected with pilocarpine to assess saliva production. As hyperglycemia may alter pulp repair, the effect of high glucose levels on the proliferation/differentiation of cultured MD10-F2 pulp cells was also analyzed. Results showed that Akita −/− mice at 6 weeks of age showed chalky white incisors that correlated with marked hyperglycemia and impaired saliva production. MicroCT of Akita −/− teeth revealed excessive enamel wearing and hypomineralization; immunostaining for Amel and Ambn was decreased. A striking feature was invasion of dentinal tubules with Streptococcus mitis and microabcesses that originated in the coronal pulp and progressed to pulp necrosis and periapical periodontitis. High levels of glucose also inhibited MD10-F2 cell proliferation and differentiation. Our findings provide the first evidence that hyperglycemia in combination with reduced saliva in a model of type1 DM leads to decreased enamel mineralization/matrix proteins and predisposes to excessive wearing and decay. Importantly, hyperglycemia adversely affects enamel matrix proteins and pulp repair. Early detection and treatment of hyperglycemia and hyposalivation may provide a useful strategy for preventing the dental complications of diabetes and promoting oral health in this population.

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Section: Resultsmentioning

confidence: 99%

Hyperglycemia and xerostomia are key determinants of tooth decay in type 1 diabetic mice

Yeh

Harris

Mohan

et al. 2012

Laboratory Investigation

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“…Analysis of subcultured bacterial colonies by matrix-assisted laser desorption/ionization-time of flight mass spectrometry offers good specificity; however, this method is expensive and lacks appropriate murine datasets. Additionally, serological tests are inappropriate for diagnosis of Pasteurellaceae infections, given that seroconversion of sentinel mice exposed to contaminated bedding or cohabitating with infected mice is unreliable [ 8 ]. There are also PCR-based options available for detection and differentiation of rodent-specific Pasteurellaceae : a genus-specific PCR assay to detect all Pasteurellaceae [ 9 ], species-specific assays to detect either of the P .…”

Section: Introductionmentioning

confidence: 99%

High-Resolution Melting Curve Analysis for Identification of Pasteurellaceae Species in Experimental Animal Facilities

2015

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Pasteurellaceae are among the most prevalent bacterial pathogens isolated from mice housed in experimental animal facilities. Reliable detection and differentiation of Pasteurellaceae are essential for high-quality health monitoring. In this study, we combined a real-time PCR assay amplifying a variable region in the 16S rRNA sequence with high-resolution melting curve analysis (HRM) to identify and differentiate among the commonly isolated species Pasteurella pneumotropica biotypes “Jawetz” and “Heyl”, Actinobacillus muris, and Haemophilus influenzaemurium. We used a set of six reference strains for assay development, with the melting profiles of these strains clearly distinguishable due to DNA sequence variations in the amplicon. For evaluation, we used real-time PCR/HRM to test 25 unknown Pasteurellaceae isolates obtained from an external diagnostic laboratory and found the results to be consistent with those of partial 16S rRNA sequencing. The real-time PCR/HRM method provides a sensitive, rapid, and closed-tube approach for Pasteurellaceae species identification for health monitoring of laboratory mice.

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“…To the best of our knowledge, experimental contact infection of P. pneumotropica in mice has not been reported previously. However, investigators have reported the transmission of P. pneumotropica [15][16].…”

Section: Discussionmentioning

confidence: 99%

“…There have been some reports of experimental intranasal inoculation models of P. pneumotropica; however, these models might not resemble natural infection [4,11,12] Experimental contact infection resembles natural infection more so than experimental inoculation models [13,14]. There have been some studies on the transmission of this species; however, to our knowledge, experimental contact infection of P. pneumotropica has not been reported [15,16]. In our previous study, NOD/shiJicscid/Jcl mice with multiple defects in adaptive and non-adaptive immunological functions were intranasally infected with bacteria [7].…”

Section: Introductionmentioning

confidence: 99%

Experimental Contact Infection of NOD/ShiJic-scid Mice with Pasteurella pneumotropica

Sasaki¹

2017

SOJMID

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Background: Pasteurella pneumotropica is a Gram-negative bacterium that infects the laboratory rodents. In immunodeficient animals, P. pneumotropica infection develops mild to severe or lethal diseases. However, little is known about the transmissibility and pathogenesis of P. pneumotropica in immunodeficient mice. In this study, to assess transmissibility and its pathological conditions by P. pneumotropica, experimental contact infection model of NOD/ShiJic-scid mice was examined with clinical and pathological approaches.

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Survival and transmissibility of Pasteurella pneumotropica

Cited by 36 publications

References 7 publications

Hyperglycemia and xerostomia are key determinants of tooth decay in type 1 diabetic mice

Hyperglycemia and xerostomia are key determinants of tooth decay in type 1 diabetic mice

High-Resolution Melting Curve Analysis for Identification of Pasteurellaceae Species in Experimental Animal Facilities

Experimental Contact Infection of NOD/ShiJic-scid Mice with Pasteurella pneumotropica

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