Survival capacity of Mycoplasma agalactiae and Mycoplasma mycoides subsp capri in the diluted semen of goat bucks and their effects on sperm quality

Gómez-Martín, Ángel; Uc, N.; Vieira, Luis Alberto; Gadea, J.; Sánchez, Antonio; Fe, Christian de la

doi:10.1016/j.theriogenology.2014.11.029

Cited by 8 publications

(14 citation statements)

References 37 publications

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“…Interestingly, the viability of M. bovis in untreated DS was seriously affected after 15 h in incubation. This is consistent with previous observations in goat DS contaminated with mycoplasmas [20]. These authors demonstrated that the acidic pH of goat ejaculate (pH ≥ 7) after dilution in the semen extender (pH ≤ 6) caused a detrimental effect on M. agalactiae and M. mycoides susbs capri.…”

Section: Discussionsupporting

confidence: 92%

“…These authors demonstrated that the acidic pH of goat ejaculate (pH ≥ 7) after dilution in the semen extender (pH ≤ 6) caused a detrimental effect on M. agalactiae and M. mycoides susbs capri. Furthermore, the glycolytic activity of sperm in the semen extender contributes to the acidification of the medium, which may negatively affect the viability of mycoplasmas present in DS [20,36]. On the other hand, we confirmed that Lactobacillus spp.…”

Section: Discussionsupporting

confidence: 68%

“…Indeed, the sensitivity of Mycoplasma agalactiae and Mycoplasma mycoides subsp. capri to the pH decreases in goat ejaculates and diluted semen was recently reported [20]. On the other hand, previous studies have demonstrated that lactic acid bacteria (LAB) of the genus, Lactobacillus spp., can inhibit the growth of human pathogenic bacteria through competition for resources, stimulation of the host immune system, and the production of hydrogen peroxide and acetic and lactic acids, which decrease pH [21,22].…”

Section: Introductionmentioning

confidence: 97%

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The Addition of Lactobacillus spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of Mycoplasma bovis in Diluted Bovine Semen

García-Galán

Gómez-Martín

Bataller

et al. 2020

Animals

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Mycoplasma bovis is an important etiologic agent of bovine mycoplasmosis in cattle. Different transmission routes have been described, including those related to reproduction. The presence of mycoplasma in semen has led to its appearance in infection-free areas through artificial insemination (AI). Semen was recently reported to be the initial source of two M. bovis mastitis outbreaks in two closed dairy herds in Finland. This questions the effectiveness of the antimicrobials currently used in semen extenders to control the pathogens in contaminated semen. They should be re-evaluated, or alternative measures to antimicrobials should be tested to obtain M. bovis-free semen. This in vitro study aimed to assess different strategies to reduce the risk of transmission of M. bovis through AI technologies. The viability of M. bovis (PG45, NCTC 10131) in bull semen diluted (DS) in a Tris-citrate-fructose solution was tested, after the addition of enrofloxacin, doxycycline or a Lactobacillus spp.-based probiotic. The data show the susceptibility of the pathogen to the addition of 0.125 μg/mL of enrofloxacin or 0.0625 μg/mL of doxycycline and to the addition of the probiotic at a concentration of 3.24 × 106 colony forming units (CFU)/mL or 3.24 × 108 CFU/mL in DS. The Tris-citrate-fructose medium negatively affected the viability of M. bovis, although this effect was lower than that observed after the addition of the probiotic and antimicrobials (p < 0.05). Our results may support new strategies for reducing the risk of M. bovis transmission through AI.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 97%

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The Addition of Lactobacillus spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of Mycoplasma bovis in Diluted Bovine Semen

García-Galán

Gómez-Martín

Bataller

et al. 2020

Animals

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“…Other authors observed a harmful effect on Mycoplasma agalactiae and Mycoplasma mycoides subsp. capri when diluting contaminated goat ejaculate in an acidic semen extender (pH ≤ 6) [24]. On the contrary, the abnormally elevated vaginal pH (> 4.5) in women with bacterial vaginosis may promote the survival of Mycoplasma genitalium [30].…”

Section: Discussionmentioning

confidence: 99%

“…Mycoplasmas lack a cell wall and therefore they are very sensitive to external changes such as pH variations [22]. In this sense, several authors have observed the effectiveness of the medium acidification to control the growth of mycoplasmas [23][24][25]. In cows, the in vivo pH of the cervical mucus is generally about 6.8 and rises to about 7.6 to 8.1 during estrus [5,26,27].…”

Section: Introductionmentioning

confidence: 99%

The addition of Lactobacillus spp. negatively affects Mycoplasma bovis viability in bovine cervical mucus

García-Galán

Gomis

et al. 2020

BMC Vet Res

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Background: Mycoplasma bovis is an important pathogen for the cattle industry worldwide causing significant economic losses. Several transmission routes, including those related to reproduction, have been described. Indeed, the pathogen can colonize the female reproductive tract after artificial insemination (AI) with contaminated semen. Lactobacillus spp.-based probiotics have been used for vaginal dysbiosis treatment in women and cows although their role in controlling cervico-vaginal infections due to M. bovis is unknown. The objective of the present work is to assess the viability of M. bovis (PG45, NCTC 10131) in experimentally contaminated cervical mucus after the addition of Lactobacillus spp. at different concentrations as a competing agent and pH acidifier. Results: The addition of probiotic at a concentration higher than 10 8 colony forming units (CFU/mL had a detrimental effect (P < 0.05) on mycoplasma viability in cervical mucus. This coincided with a significant LAB growth and an important decrease in pH from 8.4 to 5.6 (P < 0.05). However, after the addition of less concentrated probiotic, M. bovis survival was not affected and there was no significant LAB growth despite the drop of pH from 8.4 to 6.73 (P < 0.05). Conclusion: The addition of concentrations higher than 10 8 CFU/mL of Lactobacillus spp. negatively affects M. bovis viability in bovine cervical mucus under in vitro conditions. Although the effect observed on the pathogen viability seems to be related to the pH decrease after LAB proliferation in cervical mucus, further studies are necessary to elucidate if other factors are implicated. Nevertheless, the administration of Lactobacillus spp.-based probiotics might be used in the future to control M. bovis proliferation in the cervico-vaginal tract of cows.

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Presence of Mycoplasma agalactiae in semen of naturally infected asymptomatic rams

Ham

Tatay-Dualde

et al. 2016

Theriogenology

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Survival capacity of Mycoplasma agalactiae and Mycoplasma mycoides subsp capri in the diluted semen of goat bucks and their effects on sperm quality

Cited by 8 publications

References 37 publications

The Addition of Lactobacillus spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of Mycoplasma bovis in Diluted Bovine Semen

The Addition of Lactobacillus spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of Mycoplasma bovis in Diluted Bovine Semen

The addition of Lactobacillus spp. negatively affects Mycoplasma bovis viability in bovine cervical mucus

Presence of Mycoplasma agalactiae in semen of naturally infected asymptomatic rams

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