Survival, Development, and Oviposition of Resistant Diamondback Moth (Lepidoptera: Plutellidae) on Transgenic Canola Producing a Bacillus thuringiensis Toxin

Ramachandran, Suresh; Buntin, G. David; All, John; Tabashnik, Bruce E.; Raymer, Paul L.; Adang, Michael J.; Pulliam, D. A.; Stewart, C. N.

doi:10.1093/jee/91.6.1239

Cited by 78 publications

(38 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Survival to maturity has been reported for resistant strains of diamondback moth on B. thu- ringiensis-transgenic broccoli and B. thuringiensis-transgenic canola (17,22,38) and for tobacco budworm (Heliothis virescens) and pink bollworm (Pectinophora gossypiella) on B. thuringiensis-transgenic cotton (6,13). However, in all these reports the resistant strains did not develop directly from selection on B. thuringiensis-transgenic crops.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development and Characterization of Diamondback Moth Resistance to Transgenic Broccoli Expressing High Levels of Cry1C

Zhao

Collins

Tang

et al. 2000

Appl Environ Microbiol

116

View full text Add to dashboard Cite

A field-collected colony of the diamondback moth, Plutella xylostella, had 31-fold resistance to Cry1C protoxin of Bacillus thuringiensis. After 24 generations of selection with Cry1C protoxin and transgenic broccoli expressing a Cry1C protein, the resistance that developed was high enough that neonates of the resistant strain could complete their entire life cycle on transgenic broccoli expressing high levels of Cry1C. After 26 generations of selection, the resistance ratios of this strain to Cry1C protoxin were 12,400-and 63,100-fold, respectively, for the neonates and second instars by a leaf dip assay. The resistance remained stable until generation 38 (G38) under continuous selection but decreased to 235-fold at G38 when selection ceased at G28. The Cry1C resistance in this strain was seen to be inherited as an autosomal and incompletely recessive factor or factors when evaluated using a leaf dip assay and recessive when evaluated using Cry1C transgenic broccoli. Saturable binding of 125 I-Cry1C was found with brush border membrane vesicles (BBMV) from both susceptible and Cry1C-resistant strains. Significant differences in Cry1C binding to BBMV from the two strains were detected. BBMV from the resistant strain had about sevenfold-lower affinity for Cry1C and threefold-higher binding site concentration than BBMV from the susceptible strain. The overall Cry1C binding affinity was just 2.5-fold higher for BBMV from the susceptible strain than it was for BBMV from the resistant strain. These results suggest that reduced binding is not the major mechanism of resistance to Cry1C.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Laboratory populations of Cry1A-resistant diamondback moth can also survive on transgenic crucifers expressing a high level of Cry1Ac (17,22,38).…”

mentioning

confidence: 99%

Development and Characterization of Diamondback Moth Resistance to Transgenic Broccoli Expressing High Levels of Cry1C

Zhao

Collins

Tang

et al. 2000

Appl Environ Microbiol

116

View full text Add to dashboard Cite

show abstract

“…For example, resistance to Bt crops may not be recessive in some pests (42). Furthermore, field-evolved resistance to Cry1A toxins in diamondback moth, which occurred in response to Bt sprays, does not necessarily entail fitness costs or incomplete resistance on Bt plants (43,44). Unlike laboratory-selected resistance to Cry1A toxins in some strains of pink bollworm, tobacco budworm (Heliothis virescens) (45), and cotton bollworm (Helicoverpa armigera) (46), field-evolved resistance to Cry1A toxins in some strains of diamondback moth is not linked with cadherin mutations (47).…”

Section: Discussionmentioning

confidence: 99%

Delayed resistance to transgenic cotton in pink bollworm

Tabashnik

Dennehy

Carrière

2005

Proc. Natl. Acad. Sci. U.S.A.

197

181

View full text Add to dashboard Cite

Transgenic crops producing Bacillus thuringiensis (Bt) toxins kill some key insect pests and thus can reduce reliance on insecticides. Widespread planting of such Bt crops increased concerns that their usefulness would be cut short by rapid evolution of resistance to Bt toxins by pests. Pink bollworm (Pectinophora gossypiella) is a major pest that has experienced intense selection for resistance to Bt cotton in Arizona since 1997. We monitored pink bollworm resistance to Bt toxin for 8 years with laboratory bioassays of strains derived annually from 10 -17 cotton fields statewide. Bioassay results show no net increase from 1997 to 2004 in the mean frequency of pink bollworm resistance to Bt toxin. A synthesis of experimental and modeling results suggests that this delay in resistance can be explained by refuges of cotton without Bt toxin, recessive inheritance of resistance, incomplete resistance, and fitness costs associated with resistance.Bacillus thuringiensis ͉ resistance management ͉ transgenic crops

show abstract

“…Larvae from some resistant strains of diamondback moth can survive on B. thuringiensis canola or B. thuringiensis broccoli with Cry1A or Cry1C toxins (20,24,38,39), yet these transgenic plants are not grown commercially and were developed primarily for resistance research. In contrast, laboratory selection has produced strains of pink bollworm (Pectinophora gossypiella) that can complete larval development on firstgeneration B. thuringiensis cotton that is grown commercially on millions of hectares (3,16,17,32).…”

mentioning

confidence: 99%

Control of Resistant Pink Bollworm ( Pectinophora gossypiella ) by Transgenic Cotton That Produces Bacillus thuringiensis Toxin Cry2Ab

Tabashnik

Dennehy

Sims

et al. 2002

Appl Environ Microbiol

106

View full text Add to dashboard Cite

Crops genetically engineered to produce Bacillus thuringiensis toxins for insect control can reduce use of conventional insecticides, but insect resistance could limit the success of this technology. The first generation of transgenic cotton with B. thuringiensis produces a single toxin, Cry1Ac, that is highly effective against susceptible larvae of pink bollworm (Pectinophora gossypiella), a major cotton pest. To counter potential problems with resistance, second-generation transgenic cotton that produces B. thuringiensis toxin Cry2Ab alone or in combination with Cry1Ac has been developed. In greenhouse bioassays, a pink bollworm strain selected in the laboratory for resistance to Cry1Ac survived equally well on transgenic cotton with Cry1Ac and on cotton without Cry1Ac. In contrast, Cry1Ac-resistant pink bollworm had little or no survival on secondgeneration transgenic cotton with Cry2Ab alone or with Cry1Ac plus Cry2Ab. Artificial diet bioassays showed that resistance to Cry1Ac did not confer strong cross-resistance to Cry2Aa. Strains with >90% larval survival on diet with 10 g of Cry1Ac per ml showed 0% survival on diet with 3.2 or 10 g of Cry2Aa per ml. However, the average survival of larvae fed a diet with 1 g of Cry2Aa per ml was higher for Cry1Ac-resistant strains (2 to 10%) than for susceptible strains (0%). If plants with Cry1Ac plus Cry2Ab are deployed while genes that confer resistance to each of these toxins are rare, and if the inheritance of resistance to both toxins is recessive, the efficacy of transgenic cotton might be greatly extended.

show abstract

Survival, Development, and Oviposition of Resistant Diamondback Moth (Lepidoptera: Plutellidae) on Transgenic Canola Producing a Bacillus thuringiensis Toxin

Cited by 78 publications

References 0 publications

Development and Characterization of Diamondback Moth Resistance to Transgenic Broccoli Expressing High Levels of Cry1C

Development and Characterization of Diamondback Moth Resistance to Transgenic Broccoli Expressing High Levels of Cry1C

Delayed resistance to transgenic cotton in pink bollworm

Control of Resistant Pink Bollworm ( Pectinophora gossypiella ) by Transgenic Cotton That Produces Bacillus thuringiensis Toxin Cry2Ab

Contact Info

Product

Resources

About