Survival guide: Escherichia coli in the stationary phase

Pletnev, Philipp; Osterman, Ilya А.; Сергиев, Петр В.; Bøgdanov, A.; Dontsova, Olga А.

doi:10.32607/20758251-2015-7-4-22-33

Cited by 116 publications

(112 citation statements)

References 72 publications

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“…DksA was highly expressed in SESOM grown cells, suggesting a stringent response with induction of ppGpp synthesis due to nutrient limitation. DksA activated by ppGpp binds to the β-subunit of RNA polymerase, directly affecting the affinity to different promoters and thus altering the expression level of more than 80 genes, most importantly suppression of all components of the protein biosynthesis system: rRNA, ribosomal proteins, and translation factors (Pletnev et al 2015). This indicates that transcription is shut down tightly in SESOM-grown stationary phase cells.…”

Section: Discussionmentioning

confidence: 99%

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Growth and extended survival ofEscherichia coliO157:H7 in soil organic matter

NandaKafle

Christie

Vilain

et al. 2017

Preprint

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Enterohaemorrhagic Escherichia coli such as serotype O157:H7 are a leading cause of food-associated outbreaks. While the primary reservoir is associated with cattle, plant foods have been associated as sources of human infection. E. coli is able to grow in the tissue of food plants such as spinach. While fecal contamination is the primary suspect, soil has been underestimated as a potential reservoir. Persistence of bacterial populations in open systems is the product of growth, death, predation, and competition. Here we report that E. coli O157:H7 can grow using the soluble compounds in soil, and characterize the effect of soil growth in the stationary phase proteome.

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Section: Discussionmentioning

confidence: 99%

“…The elevated levels of TufA (Elongation factor Tu) in SESOM indicates minor starvation due to nutrient limitation. TufA plays an important role in a minor starvation defense mechanism where it helps in rescuing stuck ribosomes (Pletnev et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Growth and extended survival ofEscherichia coliO157:H7 in soil organic matter

NandaKafle

Christie

Vilain

et al. 2017

Preprint

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“…One of the most prominent phenotypes observed in this study was the deregulated phenotype of stationary phase Lin DYRK1 −/− mutants. Stationary phase phenotypes in unicellular organisms are known to have activated the internal systems of protection against stress and are able to survive better under adverse or changing environmental settings (Pletnev, Osterman, Sergiev, Bogdanov, & Dontsova, ). In Leishmania too, stationary phase promastigotes have increased ability to survive in the intracellular environment of the mammalian host (Wozencraft & Blackwell, ) and this ability is associated with metacyclogenesis (da Silva & Sacks, ).…”

Section: Discussionmentioning

confidence: 99%

Leishmania dual‐specificity tyrosine‐regulated kinase 1 (DYRK1) is required for sustaining Leishmania stationary phase phenotype

Rocha

Dacher

Young

et al. 2020

Molecular Microbiology

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Although the multiplicative and growth-arrested states play key roles in Leishmania development, the regulators of these transitions are largely unknown. In an attempt to gain a better understanding of these processes, we characterised one member of a family of protein kinases with dual specificity, LinDYRK1, which acts as a stasis regulator in other organisms. LinDYRK1 overexpressing parasites displayed a decrease in proliferation and in cell cycle re-entry of arrested cells. Parasites lacking LinDYRK1 displayed distinct fitness phenotypes in logarithmic and stationary growth phases. In logarithmic growth phase, LinDYRK1 -/parasites proliferated better than control lines, supporting a role of this kinase in stasis, while in stationary growth phase, LinDYRK1 -/parasites had important defects as they rounded up, accumulated vacuoles and lipid bodies and displayed subtle but consistent differences in lipid composition. Moreover, they expressed less metacyclic-enriched transcripts, displayed increased sensitivity to complement lysis and a significant reduction in survival within peritoneal macrophages. The distinct LinDYRK1 -/growth phase phenotypes were mirrored by the distinct LinDYRK1 localisations in logarithmic (mainly in flagellar pocket area and endosomes) and late stationary phase (mitochondrion).Overall, this work provides first evidence for the role of a DYRK family member in sustaining promastigote stationary phase phenotype and infectivity. K E Y W O R D SDYRK1, growth, kinase, Leishmania, stationary

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“…This, together with the fact that no violacein is produced by stationary-phase cells even in the presence of saturating amounts of AHL viol (Figure 2C), also explains how epigenetic variability is achieved ( Figure 3C). The well-known arrest of protein synthesis during stationary phase [45] should effectively prevent the accumulation of additional activator-AHL monomers in non-growing cells. Hence, cell lineages carrying lower levels of activator-AHL monomers will never reach a c .…”

Section: Discussionmentioning

confidence: 99%

A single intracellular protein governs the critical transition from an individual to a coordinated population response during quorum sensing: Origins of primordial language

Vila-Sanjurjo

Engwer

Qin

et al. 2016

Preprint

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Quorum sensing (QS) explains a type of bacterial cell-cell communication mediated by exocellular compounds that act as autoinducers (AIs). As such, QS can be considered the most primordial form of language. QS has profound implications for the control of many important traits (e.g. biofilm formation, secretion of virulence factors, etc.). Conceptually, the QS response can be split into its "listening" and "speaking" components, i.e. the power to sense AI levels vs. the ability to synthesize and release these molecules. By explaining the cell-density dependence of QS behavior as the consequence of the system's arrival to a threshold AI concentration, models of QS have traditionally assumed a salient role for the "QS speaking" module during bacterial cell-to-cell communication. In this paper, we have provided evidence that challenges this AI-centered view of QS and establishes LuxR-like activators at the center of QS. Our observation that highly coordinated, cell-density dependent responses can occur in the absence of AI production, implies that the ability to launch such responses is engrained within the "QS listening" module. Our data indicates that once a critical threshold of intracellular activator monomers in complex with AI is reached, a highly orchestrated QS response ensues. While displaying a clear cell-density dependence, such response does not strictly require the sensing of population levels by individual cells. We additionally show, both in vivo and in silico, that despite their synchronous nature, QS responses do not require that all the cells in the population participate in the response. Central to our analysis was the discovery that percolation theory (PT) can be used to mathematically describe QS responses. While groundbreaking, our results are in agreement with and integrate the latest conclusions reached in the field. We explain for the first time, the cell-density-dependent synchronicity of QS responses as the function of a single protein, the LuxR-like activator, capable of coordinating the temporal response of a population of cells in the absence of cell-to-cell communication. Being QS the most primordial form of speech, our results have important implications for the evolution of language in its ancient chemical form.Abbreviations: 3D = three dimensional, ac = threshold intracellular concentration of activator molecules, AHL = acyl-homoserine lactone, AHL f isch = N-(3-oxohexanoyl)-L-homoserine lactone, AHL viol = N-hexanoyl-DL-homoserine-lactone, AI = autoinducer, a.u. = arbitrary units, BMB = bromophenol blue, CA = trans-cinnamaldehyde, Fl = fluorescence intensity, FI/OD600 = density-normalized fluorescence intensity, GFP = green fluorescent protein, M w = molecular weight, PT = percolation theory, QS = quorum sensing, tc = percolation critical time, wt = wild type Quorum sensing (QS) explains a type of bacterial cell-to-cell communication mediated by exocellular chemical compounds that act as autoinducers (AIs). Since QS can be considered the most primordial form of language [1-6], unde...

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Survival guide: Escherichia coli in the stationary phase

Abstract: This review centers on the stationary phase of bacterial culture. The basic processes specific to the stationary phase, as well as the regulatory mechanisms that allow the bacteria to survive in conditions of stress, are described.

Cited by 116 publications

References 72 publications

Growth and extended survival ofEscherichia coliO157:H7 in soil organic matter

Growth and extended survival ofEscherichia coliO157:H7 in soil organic matter

Leishmania dual‐specificity tyrosine‐regulated kinase 1 (DYRK1) is required for sustaining Leishmania stationary phase phenotype

A single intracellular protein governs the critical transition from an individual to a coordinated population response during quorum sensing: Origins of primordial language

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