Survival of Escherichia coli O157:H7 in Soils from Jiangsu Province, China

Zhang, Taoxiang; Wang, Haizhen; Wu, Laosheng; Lou, Jun; Wu, Jianjun; Brookes, Philip C.; Xu, Jianming

doi:10.1371/journal.pone.0081178

Cited by 30 publications

(27 citation statements)

References 38 publications

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“…coli . The study comparing the acidic and neutral soils of China claimed that the acidic soils (between 5.1 to 4.6) had very shorter ttd (about 7 days), while the near neutral soils (6.5 to 7.2) had longer ttd (about 30 days) [ 20 ]. In the present study, the pH of the soils is slightly acidic (5.9 to 6.05) and alkaline (8.4 to 8.7) nature and among which the alkaline soils had quick decline of O157 than slightly acidic condition.…”

Section: Discussionmentioning

confidence: 99%

“…When the pH falls (below 5.0), the cells intended for sorption on the minerals and thereby decline their population [ 66 ]. Additionally, low available P, organic N, Al and Mn toxicity are the indirect effects cause survival and activity of O157:H7 in acid soils [ 20 , 67 ]. On the other hand, at alkaline pH (above 8.0), the Ca 2+ and Mg 2+ ions cause significant reduction of O157, which was evident by recent work [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

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Persistence of Pathogenic and Non-Pathogenic Escherichia coli Strains in Various Tropical Agricultural Soils of India

et al. 2015

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The persistence of Shiga-like toxin producing E. coli (STEC) strains in the agricultural soil creates serious threat to human health through fresh vegetables growing on them. However, the survival of STEC strains in Indian tropical soils is not yet understood thoroughly. Additionally how the survival of STEC strain in soil diverges with non-pathogenic and genetically modified E. coli strains is also not yet assessed. Hence in the present study, the survival pattern of STEC strain (O157-TNAU) was compared with non-pathogenic (MTCC433) and genetically modified (DH5α) strains on different tropical agricultural soils and on a vegetable growing medium, cocopeat under controlled condition. The survival pattern clearly discriminated DH5α from MTCC433 and O157-TNAU, which had shorter life (40 days) than those compared (60 days). Similarly, among the soils assessed, the red laterite and tropical latosol supported longer survival of O157-TNAU and MTCC433 as compared to wetland and black cotton soils. In cocopeat, O157 recorded significantly longer survival than other two strains. The survival data were successfully analyzed using Double-Weibull model and the modeling parameters were correlated with soil physico-chemical and biological properties using principal component analysis (PCA). The PCA of all the three strains revealed that pH, microbial biomass carbon, dehydrogenase activity and available N and P contents of the soil decided the survival of E. coli strains in those soils and cocopeat. The present research work suggests that the survival of O157 differs in tropical Indian soils due to varied physico-chemical and biological properties and the survival is much shorter than those reported in temperate soils. As the survival pattern of non-pathogenic strain, MTCC433 is similar to O157-TNAU in tropical soils, the former can be used as safe model organism for open field studies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Persistence of Pathogenic and Non-Pathogenic Escherichia coli Strains in Various Tropical Agricultural Soils of India

et al. 2015

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“…The boundaries of E. coli survival in secondary environments are wide. It can survive in cow manure at levels of circa 5 Â 10 7 cells/gram of soil for up to 15 days [84]; in strongly acidic soils (pH 4.57 to 5.14) it survives for up to 7 days; and in more neutral soils (pH 6.51 to 7.39) it can last up to 35 days [85]. In the laboratory, E. coli can also breach the biotic resistance of the establishment phase, and this has been shown when resources are ample and biotic counterpressure is low [26].…”

Section: •Compeɵɵon •Antagonism •Predaɵonmentioning

confidence: 99%

Microbial Invasions: The Process, Patterns, and Mechanisms

Mallon

Elsas

Salles

2015

Trends in Microbiology

393

354

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“…Another study performed in the same year estimated that foodborne pathogens resulted in medical care costs in the range from $4.4 billion to $33.0 billion (2). Contamination of soil and water leading to foodborne illness can originate from numerous sources, such as direct deposition by animals, overflow or leakage of faulty septic and sewage systems, or manure application to agricultural fields and pastures (3)(4)(5). The potential presence of pathogens that cause foodborne illness can be inferred via the monitoring of fecal indicator bacteria (FIB), such as Escherichia coli and enterococci (6).…”

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confidence: 99%

Depth-Dependent Survival of Escherichia coli and Enterococci in Soil after Manure Application and Simulated Rainfall

Stocker

Pachepsky

Hill

et al. 2015

Appl Environ Microbiol

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bOnce released, manure-borne bacteria can enter runoff via interaction with the thin mixing layer near the soil surface. The objectives of this work were to document temporal changes in profile distributions of manure-borne Escherichia coli and enterococci in the nearsurface soil layers after simulated rainfalls and to examine differences in survival of the two fecal indicator bacteria. Rainfall simulations were performed in triplicate on soil-filled boxes with grass cover and solid manure application for 1 h with rainfall depths of 30, 60, and 90 mm. Soil samples were collected weekly from depth ranges of 0 to 1, 1 to 2, 2 to 5, and 5 to 10 cm for 1 month. Rainfall intensity was found to have a significant impact on the initial concentrations of fecal indicator bacteria in the soil. While total numbers of enterococci rapidly declined over time, E. coli populations experienced initial growth with concentration increases of 4, 10, and 25 times the initial levels at rainfall treatment depths of 30, 60, and 90 mm, respectively. E. coli populations grew to the approximately the same level in all treatments. The 0-to 1-cm layer contained more indicator bacteria than the layers beneath it, and survival of indicator bacteria was better in this layer, with decimation times between 12 and 18 days after the first week of growth. The proportion of bacteria in the 0-to 1-cm layer grew with time as the total number of bacteria in the 0-to 10-cm layer declined. The results of this work indicate the need to revisit the bacterial survival patterns that are assumed in water quality models. Fecal contamination of soil and water presents a major worldwide health risk. When pathogenic microorganisms enter soil or water, they can infect humans via drinking or recreational activities and through consumption or handling of contaminated produce. The Centers for Disease Control and Prevention (1) estimated in 2011 that each year nearly 1 in 6 Americans (or 46 million people) get sick and roughly 3,000 die from foodborne diseases. Another study performed in the same year estimated that foodborne pathogens resulted in medical care costs in the range from $4.4 billion to $33.0 billion (2). Contamination of soil and water leading to foodborne illness can originate from numerous sources, such as direct deposition by animals, overflow or leakage of faulty septic and sewage systems, or manure application to agricultural fields and pastures (3-5). The potential presence of pathogens that cause foodborne illness can be inferred via the monitoring of fecal indicator bacteria (FIB), such as Escherichia coli and enterococci (6).It has been found that both E. coli and enterococci can survive in the soil for several weeks after deposition, if not longer (7,8). Some studies have found that populations of these organisms can experience initial growth in the soil that can extend their prevalence for longer than previously thought. For example, Lau and Ingham (9) reported that E. coli and enterococci survived in soil following manure incorporation for mo...

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Survival of Escherichia coli O157:H7 in Soils from Jiangsu Province, China

Cited by 30 publications

References 38 publications

Persistence of Pathogenic and Non-Pathogenic Escherichia coli Strains in Various Tropical Agricultural Soils of India

Persistence of Pathogenic and Non-Pathogenic Escherichia coli Strains in Various Tropical Agricultural Soils of India

Microbial Invasions: The Process, Patterns, and Mechanisms

Depth-Dependent Survival of Escherichia coli and Enterococci in Soil after Manure Application and Simulated Rainfall

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