Survival of <i>Xanthomonas axonopodis</i> pv. <i>citri</i> in Leaf Lesions Under Tropical Environmental Conditions and Simulated Splash Dispersal of Inoculum

Pruvost, Olivier; Boher, Bernard; Brocherieux, Christophe; Nicole, Michel; Chiroleu, Frédéric

doi:10.1094/phyto.2002.92.4.336

Cited by 65 publications

(63 citation statements)

References 27 publications

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“…Studies have shown that the optimal temperature for disease development is 25°-30°C (Pruvost et al, 2002). HIS CPXAC enzyme exhibited optimal activity at 30°C, which agrees with the best temperature for infection.…”

Section: Discussionmentioning

confidence: 52%

“…HIS CPXAC enzyme exhibited optimal activity at 30°C, which agrees with the best temperature for infection. According to studies in subtropical environmental conditions, Xac cells remain viable for a long period around the lesion (latent form) at temperatures of approximately 20°C; moreover, a rapid decline in bacterial population has been observed at low temperatures, which explains the minimal activity of the enzyme at 10°C (Pruvost et al, 2002;Dalla Pria et al, 2006). This information agrees with our observed data on HIS CPXAC activity at various temperatures.…”

Section: Discussionmentioning

confidence: 99%

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Recombinant expression and characterization of a cysteine peptidase from Xanthomonas citri subsp citri

Soares-Costa¹,

Silveira²,

Novo³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Xanthomonas citri subsp citri (Xac) is the bacterium responsible for citrus canker disease in citrus plants. The aim of this study was to describe the recombinant expression, purification, and characterization of a cysteine peptidase from Xac strain 306, which is a candidate for involvement in the pathogenicity of this bacterium. The gene was cloned and expressed in Pichia pastoris, and the cysteine peptidase was successfully expressed, secreted, and purified using affinity chromatography with a yield of approximately 10 mg/L. A polyclonal antibody produced against cysteine peptidase from X. citri subsp citri fused with HIS tag ( HIS CPXAC) recognized the purified recombinant cysteine peptidase HIS CPXAC, confirming the correct production of this protein in P. pastoris. The same antibody detected the protein in the culture supernatant of Xac grown in pathogenicityinducing medium. Kinetic analysis revealed that HIS CPXAC hydrolyzed the carbobenzoxy-Leu-Arg-7-amido-4-methylcoumarin substrate with a catalytic efficiency (k cat /K m ) of 47 μM -1 •s -1 . The purified HIS CPXAC displayed maximal catalytic activity at pH 5.5 and 30°C. The recombinant enzyme was inhibited by the specific cysteine peptidase inhibitor E-64, as well as by the recombinant cysteine peptidase inhibitors CaneCPI-1, CaneCPI-2, CaneCPI-3, and CaneCPI-4, with K i values of 1.214, 84.64, 0.09, 0.09, and 0.012 nM, respectively. Finally, the N-terminal sequencing of the purified protein enabled the identification of the first 5 amino acid residues (AVHGM) immediately after the putative signal peptide, thereby enabling the identification of the cleavage point and corroborating previous studies that have identified this sequence in a secreted protein from Xanthomonas spp.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Recombinant expression and characterization of a cysteine peptidase from Xanthomonas citri subsp citri

Soares-Costa¹,

Silveira²,

Novo³

et al. 2012

Genet. Mol. Res.

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“…According to the literature (Peltier, 1920;Koizumi, 1977;Timmer et al, 1991;Pruvost et al, 2002;Bock et al, 2005), the quantity of Xanthomonas citri subsp. citri cells dispersed in rain splash depends on various factors, such as the age of the lesions and the ambient temperature.…”

Section: Dr Gottwald Et Al's Answer (12)mentioning

confidence: 99%

Statement in response to the comments submitted by the US phytosanitary authorities on a previous EFSA opinion (EFSA Journal 2011;9(12):2461) regarding the export of Florida citrus fruit to the EU

2013

EFS2

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The European Food Safety Authority (EFSA) has been asked to address the comments submitted in April 2012 by the US phytosanitary authorities on the EFSA scientific opinion on the US request related to the export of Florida citrus fruit. The EFSA Panel on Plant Health acknowledges the responses provided by the United States Department of Agriculture (USDA) and considers that the USDA misinterpreted the conclusions made by the Panel in its previous opinion (EFSA Panel on Plant Health (PLH), 2011). The Panel considers that the extrapolations of the data provided by Shiotani et al. (2009) andGottwald et al. (2009) were not supported by sound evidence. The Panel considers that both the responses provided by Dr Shiotani and by Dr Gottwald and colleagues do not lead to any major change in the set of scientifically sound information related to citrus canker and Xanthomonas citri pv. citri. The Panel, after evaluating the response provided by the USDA regarding the previous EFSA scientific opinion on citrus canker, considers that the removal of mandatory mitigation measures in field and packinghouse facilities increases the risk of contamination of fruit during the growing season and thus the bacterial load and frequency of infected fruit in consignments. Permitting the trade of citrus fruit originating from infected orchards increases the probability of the presence of X. citri pv. citri on fruit in consignments from those sites. The Panel considers that citrus fruit is a pathway for introduction of X. citri pv. citri into the European Union and the related risk is being evaluated in a separate EFSA opinion under preparation on the plant health risk of citrus canker for the EU. The Panel, after evaluating the response provided by the USDA regarding its previous pest risk assessments and risk management analyses, further considers that:removing mandatory mitigation measures in the field increases the risk of contamination of fruit during the growing season and thus the bacterial load and frequency of infected fruit in consignments;removing mandatory mitigation measures in packinghouse facilities contributes to increasing the bacterial load and frequency of infected fruit in consignments;allowing the trade of citrus fruit originating from infected orchards increases the probability of the presence of Xanthomonas citri pv. citri on fruit in consignments from those sites.The Panel considers that citrus fruit is a pathway for the introduction of X. citri pv. citri to the European Union and the related risk is being evaluated in a separate EFSA opinion under preparation on the plant health risk of citrus canker for the EU, addressing another question from the same terms of reference.Statement in response to the comments submitted by the US phytosanitary authorities EFSA Journal 2013;11(10):3374 3

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“…Effective survival of X. citri subsp. citri inoculum in cankers on leaves under tropical conditions has been demonstrated (Pruvost et al 2002).…”

mentioning

confidence: 99%

First record of citrus canker, caused by Xanthomonas citri subsp. citri in Solomon Islands

Davis¹,

Taylor

Rouse

et al. 2015

Australasian Plant Dis. Notes

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Citrus canker disease was detected for the first time in Solomon Islands on Guadalcanal Island. The bacterium was isolated from diseased citrus plants exhibiting distinct citrus canker symptoms consisting of crater-like lesions on the rind of fruit and corky yellow-brownish lesions on leaves. Xanthomonas citri subsp. citri was identified using biochemical and molecular tests and confirmed as the causal agent by fulfilling Koch's postulates.

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Survival of Xanthomonas axonopodis pv. citri in Leaf Lesions Under Tropical Environmental Conditions and Simulated Splash Dispersal of Inoculum

Cited by 65 publications

References 27 publications

Recombinant expression and characterization of a cysteine peptidase from Xanthomonas citri subsp citri

Recombinant expression and characterization of a cysteine peptidase from Xanthomonas citri subsp citri

Statement in response to the comments submitted by the US phytosanitary authorities on a previous EFSA opinion (EFSA Journal 2011;9(12):2461) regarding the export of Florida citrus fruit to the EU

First record of citrus canker, caused by Xanthomonas citri subsp. citri in Solomon Islands

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