Survival of Îº-carrageenan-encapsulated and unencapsulated Pseudomonas aeruginosa UG2Lr cells in forest soil monitored by polymerase chain reaction and spread plating

Leung, Kamtin; Cassidy, Mike; Holmes, Steve B.; Lee, Hung; Trevors, J. T.

doi:10.1111/j.1574-6941.1995.tb00270.x

Cited by 12 publications

(4 citation statements)

References 39 publications

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“…Similar results were observed in soil slurry microcosms, although the effect of prolonged biodegradation of lindane was less pronounced (extension of 1.4 times the period of degradation activity), which is most probably related to a low level of bioavailable lindane in the soil slurry relative to the aqueous solution (7). Previous research has shown that encapsulation often increases the rate of survival of the introduced microbial inoculum (19,26,33). Results showed that initially, Sphingomonas sp.…”

Section: Discussionsupporting

confidence: 74%

Slow-Release Inoculation Allows Sustained Biodegradation of γ-Hexachlorocyclohexane

Mertens

Boon

Verstraete

2006

Appl Environ Microbiol

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Section: Discussionsupporting

confidence: 74%

Slow-Release Inoculation Allows Sustained Biodegradation of γ-Hexachlorocyclohexane

Mertens

Boon

Verstraete

2006

Appl Environ Microbiol

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“…As mentioned previously, these stimuli may not be applicable to resuscitate all species or even the same species in different trials because of other interacting factors. For instance, temperature upshift can resuscitate VBNC cells of Pseudomonas aeruginosa (Leung et al, 1995) but not A. butzleri and Helicobacter pylori (Adams et al, 2003; Fera et al, 2008). Rich medium resuscitated C. jejuni in the study of Cools et al (2003) but not in other studies (Chaveerach et al, 2003; Cook and Bolster, 2007).…”

Section: Resuscitation Of Vbnc Cellsmentioning

confidence: 99%

The importance of the viable but non-culturable state in human bacterial pathogens

et al. 2014

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Many bacterial species have been found to exist in a viable but non-culturable (VBNC) state since its discovery in 1982. VBNC cells are characterized by a loss of culturability on routine agar, which impairs their detection by conventional plate count techniques. This leads to an underestimation of total viable cells in environmental or clinical samples, and thus poses a risk to public health. In this review, we present recent findings on the VBNC state of human bacterial pathogens. The characteristics of VBNC cells, including the similarities and differences to viable, culturable cells and dead cells, and different detection methods are discussed. Exposure to various stresses can induce the VBNC state, and VBNC cells may be resuscitated back to culturable cells under suitable stimuli. The conditions that trigger the induction of the VBNC state and resuscitation from it are summarized and the mechanisms underlying these two processes are discussed. Last but not least, the significance of VBNC cells and their potential influence on human health are also reviewed.

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“…Therefore, being in a VBNC state does not mean the cells have lost their ability to become virulent. It has been suggested that temperature decreases in aquatic environments are one of the most common inducers of the VBNC state in P. aeruginosa . , We therefore propose that the decrease in temperature experienced by P. aeruginosa in our study induced the VBNC state in a subpopulation of cells in all treatments. Neither the growth rate nor the maximum OD 600 were significantly different between the four conditions after 24 h of incubation (Figure ).…”

Section: Resultsmentioning

confidence: 55%

Exposure to Freeze–Thaw Conditions Increases Virulence ofPseudomonas aeruginosatoDrosophila melanogaster

Hakimzadeh

Okshevsky

Maisuria

et al. 2018

Environ. Sci. Technol.

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Groundwater contamination by pathogenic bacteria present in land-applied manure poses a threat to public health. In cold climate regions, surface soil layers experience repeated temperature fluctuations around the freezing point known as freeze−thaw (FT) cycles. With global climate change, annual soil FT cycles have increased, and this trend is expected to continue. It is therefore of interest to understand how FT cycles impact soil microbial communities. This study investigates the influence of FT cycles on the growth, culturability, biofilm formation, and virulence of the bacterial opportunistic pathogen Pseudomonas aeruginosa, a ubiquitous bacterium found in soil and water, responsible for infections in immunocompromised hosts. Our findings demonstrate that exposure to FT had no significant effect on growth or culturability of the bacteria. However, FT treatment significantly increased biofilm formation and delayed the onset of swimming motility, factors that are important for the pathogenicity of P. aeruginosa. An in vivo study using a chronic infection model revealed an increase in the virulence of P. aeruginosa after FT exposure. These results suggest that the impact of climate change on natural FT cycles may be affecting the ecology of soil-borne pathogens and host−pathogen interactions in unexpected ways.

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Survival of Îº-carrageenan-encapsulated and unencapsulated Pseudomonas aeruginosa UG2Lr cells in forest soil monitored by polymerase chain reaction and spread plating

Cited by 12 publications

References 39 publications

Slow-Release Inoculation Allows Sustained Biodegradation of γ-Hexachlorocyclohexane

Slow-Release Inoculation Allows Sustained Biodegradation of γ-Hexachlorocyclohexane

The importance of the viable but non-culturable state in human bacterial pathogens

Exposure to Freeze–Thaw Conditions Increases Virulence ofPseudomonas aeruginosatoDrosophila melanogaster

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