Survival of Pantoea agglomerans Strain CPA-2 in a Spray-Drying Process

Costa, E.; Teixidó, Neus; Usall, J.; Fons, Estanislau; Gimeno, Vicente Andrés; Delgado, Josué; Viñas, Immaculada

doi:10.4315/0362-028x-65.1.185

Cited by 41 publications

(40 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Teixidó et al, 2001;Nunes et al, 2001Nunes et al, , 2002aTorres et al, 2007). Other work related to the modes of action of this strain and to its production and viability has also been published (Costa et al, 2000(Costa et al, , 2001(Costa et al, , 2002aPoppe et al, 2003). This indicates that P. agglomerans strain CPA-2 could serve as the basis of a new biocontrol product.…”

Section: Discussionmentioning

confidence: 91%

Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2

Nunes

Bajji

Stepien

et al. 2008

Postharvest Biology and Technology

View full text Add to dashboard Cite

Pantoea agglomerans CPA-2 is an effective biocontrol agent of postharvest diseases of citrus and pome fruit. A monitoring technique was developed for its identification and to quantify its populations. The methodology used consisted of (i) searching for a semi-selective medium, (ii) identification of molecular markers and (iii) monitoring population dynamics in a commercial trial. As a semi-selective medium, Malonate Broth Agar supplemented with tetracycline hydroxychloride and incubation at high temperature (max. of 40 • C) facilitated the selective recovery of P. agglomerans CPA-2 colonies. The RAPD technique was applied to a collection of 13 strains of P. agglomerans, including CPA-2. Among the 12 primers tested, OPL-11 amplified a fragment (about 720 bp) specific to strain CPA-2. On the basis of this fragment, two SCAR markers were amplified using a primer pair derived from OPL-11 elongation. A first SCAR marker of 720 bp was specifically amplified for the strain CPA-2 and a second one of 270 bp was obtained for all P. agglomerans strains tested, including CPA-2. Commercial trials demonstrated a significant reduction of decay with the treatment of formulated cells of P. agglomerans CPA-2. Population dynamics of CPA-2 in commercial trials were determined on fruit surfaces and in the environment using both the classical plating technique and PCR with SCAR primers. In general, no significant differences were observed between results obtained from the two methods. On fruit surfaces, 1 day after CPA-2 applied its population by classical methods was 4.37 × 10 6 cfu wound −1 and at the end of the experiment the population increased to 5.8 × 10 5 cfu wound −1 . The percentages of colonies identified as P. agglomerans CPA-2 at these sampling times using SCAR primers were 90 and 95%, respectively. Population dynamics in the environment to evaluate the environmental fate of P. agglomerans CPA-2 showed that it has a limited persistence and limited capacity for dispersion.

show abstract

Section: Discussionmentioning

confidence: 91%

Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2

Nunes

Bajji

Stepien

et al. 2008

Postharvest Biology and Technology

View full text Add to dashboard Cite

show abstract

“…Abadias et al (2007), usaron leche descremada al 10%, mejorando la sobrevivencia de la levadura Candida sake. Asimismo, Costa et al (2002), obtuvieron…”

Section: Resultados Y Discusionunclassified

Evaluación de la Estabilidad y Viabilidad de Dos Cepas Probióticas Microencapsuladas por Lecho Fluidizado

Cayra¹,

Dávila²,

Villalta³

et al. 2017

Inf. tecnol.

View full text Add to dashboard Cite

“…Spray drying is an economical but harsh way of concentrating and drying biomass and so far cellular survival have, for the most part, been poor (Abadias et al 2005;Costa et al 2002). The more robust yeast species S. cerevisiae has been successfully dehydrated using spray-drying techniques (Luna-Solano et al 2005).…”

Section: Formulation Of Microorganismsmentioning

confidence: 99%

Biological preservation of plant derived animal feed with antifungal microorganisms: safety and formulation aspects

et al. 2007

View full text Add to dashboard Cite

During storage of moist animal feed, growth of detrimental fungi causing spoilage, or being mycotoxigenic or pathogenic, is a severe problem. Addition of biopreservative yeasts or lactic acid bacteria can significantly reduce this problem. However, their use requires several careful considerations. One is the safety to the animal, humans and the environment, tightly connected to legal aspects and the need for pre-market authorisation when supplementing feed with microorganisms. Although both yeasts and lactic acid bacteria are considered comparatively safe organisms due to low production of toxic metabolites, it is of great importance to understand the mechanisms behind the biopreservative abilities. Another important issue concerns practical aspects, such as the economic production of large amounts of the organisms and the development of a suitable formulation giving the organisms a long shelf life. These aspects are discussed and a recommendation of this review is that both safety and formulation aspects of a specific microbe should be considered at an early stage in the selection of new organisms with biopreservation potential.

show abstract

Survival of Pantoea agglomerans Strain CPA-2 in a Spray-Drying Process

Cited by 41 publications

References 17 publications

Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2

Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2

Evaluación de la Estabilidad y Viabilidad de Dos Cepas Probióticas Microencapsuladas por Lecho Fluidizado

Biological preservation of plant derived animal feed with antifungal microorganisms: safety and formulation aspects

Contact Info

Product

Resources

About