Survival of rabbit embryos after culture or culture/freezing

Techakumphu, Mongkol; Wintenberger-Torrès, Suzanne; Sevellec, Claude; Ménézo, Yves

doi:10.1016/0378-4320(87)90111-4

Cited by 9 publications

(7 citation statements)

References 18 publications

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“…Furthermore, excessive pressure generated during VS removing through NM pores could induce tension over embryo surface, generating also mechanical remodeling of the mucin coat. Thus, due to most of the damage during rabbit embryo cryopreservation occurred on the mucin coat without damaging the blastomeres [23], NM vitrified embryos has competent in vitro developmental rates. However, it fails significantly during in vivo development at term, where intact mucin coat is essential for implantation.…”

Section: Discussionmentioning

confidence: 99%

The harmful effect of removing the extracellular vitrification medium during embryo cryopreservation using a nylon mesh device in rabbit

et al. 2020

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During the last decades, many techniques have been developed to reduce sample volume and improve cooling and warming rates during embryo vitrification. The vast majority are based on the "minimum drop size" concept, in which the vitrification solution around embryos is reduced by aspiration, leaving a tiny part of volume surrounding embryos.However, novel cryodevices were aimed to remove the entire vitrification solution. This study was designed to compare the "minimum drop size" technique using Cryotop® with the nylon mesh as cryodevice on rabbit morula embryos. The outcomes assessed were the in vitro development rates (experiment 1) and the offspring rates at birth (experiment 2).Embryos were vitrified in a two-step procedure; equilibrium (10% EG + 10% Me2SO) for 2 min and vitrification (20% EG + 20% Me2SO) for 1 minutes. In experiment 1, embryos (n= 323) were warmed and subsequently in vitro cultured for 48 h to assess the embryo developmental capability to reach the hatching-hatched blastocyst stage. In experiment 2, embryos were transferred using the laparoscopic technique (n=369) to assess the offspring rate at birth. In this context, rates of in vitro embryo development were similar between vitrified groups (0.73±0.042% and 0.66±0.047% for Cryotop® and nylon mesh device, respectively), but lower than in the fresh group (0.97±0.016%, p<0.05). In experiment 2, there were no significant differences in survival rates (offspring born/total embryos transferred) among the Cryotop® device group and fresh group (0.41±0.049% and 0.49±0.050%, respectively). But significantly lower value was obtained in the nylon mesh device group (0.18±0.030%). These results indicate that nylon mesh is not suitable as cryodevice for rabbit morula vitrification, remaining those using the "minimum drop size" methodology as the best option.

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Section: Discussionmentioning

confidence: 99%

The harmful effect of removing the extracellular vitrification medium during embryo cryopreservation using a nylon mesh device in rabbit

et al. 2020

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“…SMORAG et al (1989) obtained high rates of in witro development after vitrification in later stages (morula and blastocyst) and reduced viability in 1 to 2-cell embryos. This effect was initially related to reduced thickness of mucine layer in early stages of rabbit embryos due to a thickness-related protector effect of the layer (TECHAKUMPHU et al, 1987;SMORAG et al, 1989). However, rabbit morulae without a mucine layer developed in witro after vitrification, though intact morulae showed higher developmental rate (KASAI et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Development In Vitro of Rabbit Embryos After Freezing by Two‐Step or Ultrarapid Cooling Methods

López-Béjar

López‐Gatius

Camón

et al. 1994

Journal of Veterinary Medicine Series A

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Summary The effects of rapid freezing by two‐step and ultrarapid cooling methods on 2‐cell, 8 to 16‐cell, compacted morula and early blastocyst stages of rabbit embryos were examined. Dimethylsulfoxide (DMSO, 3.5mol/l) combined with sucrose (0.25mol/l) was used as the freezing medium. The embryos were loaded into plastic straws with the freezing medium, held during 2.5 min and then were directly plunged into liquid nitrogen (ultrarapid cooling) or after a time from 30 to 45 min held at — 27 °C (two‐step cooling). After rapid thawing embryo development was evaluated by in vitro developmental capacity shown by the embryos and was compared to control. All studied embryonic stages developed in vitro after two‐step and ultrarapid cooling procedures. However, higher developmental rates were obtained when the two‐step cooling method was used. Using the two‐step cooling method, best results were obtained at compacted morula and blastocyst stages, and no significant differences were shown when compared with control embryos at blastocyst stage. All embryonic stages to which the ultrarapid cooling method was applied showed lower developmental rates than control. The results show that a high proportion of rabbit embryos can develop in vitro after freezing by two‐step cooling method.

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“…In general, mammalian embryos cultured in vitro have been said to exhibit slower embryonic development than those developed in the uterus, resulting in a lowering of their viability (Smorag & Gajda, 1991). This has been used to explain a reduced ability of cultured embryos to survive cryopreservation (Techakumphu et al, 1987). The total cell number in pig blastocysts cultured in vitro was smaller than in those recovered from the uterus (Papaioannou & Ebert, 1986); the number of inner cell mass (ICM) cells and their proportion in bovine blastocysts fertilised and cultured in vitro were lower than in those fertilised in vivo (Iwasaki et al, 1990); and the cell number of mouse blastocysts transferred to mouse uteri increased more rapidly than did the cell number in embryos cultured in vitro (Bowman & McLaren, 1970).…”

Section: Introductionmentioning

confidence: 99%

Vitrification of rat blastocysts developed in vitro

Nakamichi

Ohboshi

Fujihara

1993

Zygote

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SummaryApproximately 50% of rat 8-cell embryos obtained from the oviduct on day 4 of pregnancy developed to the balstocyst stage after 18 h of incubation in vitro. The embroys were compared with in vivo blastocysts derived from the uterus on day 5 of pregnancy as regards their response to vitrification treatment. Before vitrification, both types of embryos were exposed to vitrification solution, and subsequent embryonic development was inhibited with increasing time of exposure. A greater suppression of development after exposure was observed in the embryos cultured in vitro compared with in vivo blastocysts. However, development was gradually restored as in vitro incubation was continued. The response of embryos to vitrification and warming treatments was shown to be almost the same for both in vitro and in vivo blastocysts, though the former were significantly (p < 0.05) less tolerant of exposure to vitrification solution. These results suggest that in vitro blastocysts were much more susceptible to vitrification solution than in vivo blastocysts. The degree of damage caused by vitrification and thawing treatments, and the inhibition of in vitro embryonic development, were significantly (p < 0.05) more serious for in vitro blastocysts.

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Survival of rabbit embryos after culture or culture/freezing

Cited by 9 publications

References 18 publications

The harmful effect of removing the extracellular vitrification medium during embryo cryopreservation using a nylon mesh device in rabbit

The harmful effect of removing the extracellular vitrification medium during embryo cryopreservation using a nylon mesh device in rabbit

Development In Vitro of Rabbit Embryos After Freezing by Two‐Step or Ultrarapid Cooling Methods

Vitrification of rat blastocysts developed in vitro

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