Survival of silk worm, Bombyx mori in azaserine induced oxidative stress

Mandyam.D., Venkatesh; Subramanyam, M. V. V.

doi:10.1016/j.cbpc.2019.108637

Cited by 1 publication

(2 citation statements)

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“…36 The treatment of silk glands with caspase inhibitors can reduce the expression of apoptosis-related genes and thereby delay the apoptosis of silk glands, 37 while oxidative stress stimulates apoptosis cascade reaction. 38 Silk is mainly composed of silk fibroin and sericin, with the latter accounting for about 25-30% of the total mass of cocoon silk. 34 The main genes for silk protein synthesis, BmSer1, BmSer2 and BmSer3, are tissue-specifically expressed in the middle silk gland.…”

Section: Introductionmentioning

confidence: 99%

“…Exogenous 20E promotes programmed cell death in insects 36 . The treatment of silk glands with caspase inhibitors can reduce the expression of apoptosis‐related genes and thereby delay the apoptosis of silk glands, 37 while oxidative stress stimulates apoptosis cascade reaction 38 . Silk is mainly composed of silk fibroin and sericin, with the latter accounting for about 25–30% of the total mass of cocoon silk 34 .…”

Section: Introductionmentioning

confidence: 99%

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Cold‐stored mulberry leaves affect antioxidant system and silk proteins of silkworm (Bombyx mori) larva

2023

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BACKGROUNDCold storage has been widely used to maintain the quality of vegetables, but whether eating cold‐stored vegetables affects health remains unknown.RESULTSThis study used silkworms as an animal model to evaluate the effects of nutrient changes in cold‐stored mulberry leaves (CSML) on health. Compared with fresh mulberry leaves (FML), CSML contained lower vitamin C, soluble sugars and proteins, and higher H2O2, suggesting decreased antioxidant ability and nutrition. The CSML did not obviously affect larval survival rate, body weight or dry matter rate, cocoon shape, weight and size, or final rates of cluster and cocooning relative to the FML, suggesting CSML did not alter overall growth and development. However, the CSML increased the initial rates of cluster and cocooning and upregulated BmRpd3, suggesting CSML shortened larval lifespan and enhanced senescence. CSML upregulated BmNOX4, downregulated BmCAT, BmSOD and BmGSH‐Px and increased H2O2 in silkworms, suggesting CSML caused oxidative stress. CSML upregulated ecdysone biosynthesis and inactivation genes and elevated ecdysone concentration in silkworms, suggesting that CSML affected hormone homeostasis. CSML upregulated apoptosis‐related genes, downregulated sericin and silk fibroin genes and decreased sericin content rate in silkworms, suggesting oxidative stress and protein deficiency.CONCLUSIONCold storage reduced nutrition and antioxidant capability of mulberry leaves. CSML did not influence growth and development of silkworm larva, but affected health by causing oxidative stress and reducing protein synthesis. The findings show that the ingredient changes in CSML had negative effects on health of silkworms. © 2023 Society of Chemical Industry.

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