Survival of the Aerobic Denitrifier<i>Pseudomonas stutzeri</i>Strain TR2 during Co-Culture with Activated Sludge under Denitrifying Conditions

Miyahara, Morio; Kim, Sang Wan; Zhou, Shengmin; Fushinobu, Shinya; Yamada, Takeshi; Ikeda-Ohtsubo, Wakako; Watanabe, Akira; Miyauchi, Keisuke; Endo, Ginro; Wakagi, Takayoshi; Shoun, Hirofumi

doi:10.1271/bbb.110785

Cited by 11 publications

(3 citation statements)

References 36 publications

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“…Pseudomonas stutzeri , a model denitrifying pseudomonas, can grow in diverse conditions ( Lalucat et al, 2006 ) and its use has been demonstrated in a number of bioremediation processes, including phenol ( Jiang et al, 2014 ), carbon tetrachloride ( Sepúlveda-Torres et al, 2002 ), uranium ( Han et al, 2012 ), polycyclic aromatic hydrocarbons ( Moscoso et al, 2012a ), and diesel oil ( Kaczorek et al, 2012 ). Additionally, P. stutzeri has been identified in and used in a number of consortia-based applications, such as bioremediation ( Nguyen et al, 2008 ; Brodie et al, 2011 ; Gałazka et al, 2012 ), wastewater denitrification ( Clarens et al, 1998 ; Liu et al, 2006 ), aquaculture water quality management ( Erna et al, 2013 ; Deng et al, 2014 ), as a plant growth promoter for the biocontrol of phytopathogens ( Cottyn et al, 2009 ; Shen et al, 2013 ), and manufacturing/municipal waste management ( Miyahara et al, 2012 ; Moscoso et al, 2012b ). The use of P. stutzeri biofilms for various bioremediation efforts has potential benefits in terms of activity and yields for copper removal ( Badar et al, 2013 ), drinking water denitrification ( Lazarova et al, 1992 ) and naphthalene ( Zhao et al, 2016 ), and phenol ( Viggiani et al, 2006 ) degradation.…”

Section: Introductionmentioning

confidence: 99%

Biofilm Interaction Mapping and Analysis (BIMA) of Interspecific Interactions in Pseudomonas Co-culture Biofilms

et al. 2021

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Pseudomonas species are ubiquitous in nature and include numerous medically, agriculturally and technologically beneficial strains of which the interspecific interactions are of great interest for biotechnologies. Specifically, co-cultures containing Pseudomonas stutzeri have been used for bioremediation, biocontrol, aquaculture management and wastewater denitrification. Furthermore, the use of P. stutzeri biofilms, in combination with consortia-based approaches, may offer advantages for these processes. Understanding the interspecific interaction within biofilm co-cultures or consortia provides a means for improvement of current technologies. However, the investigation of biofilm-based consortia has been limited. We present an adaptable and scalable method for the analysis of macroscopic interactions (colony morphology, inhibition, and invasion) between colony-forming bacterial strains using an automated printing method followed by analysis of the genes and metabolites involved in the interactions. Using Biofilm Interaction Mapping and Analysis (BIMA), these interactions were investigated between P. stutzeri strain RCH2, a denitrifier isolated from chromium (VI) contaminated soil, and 13 other species of pseudomonas isolated from non-contaminated soil. One interaction partner, Pseudomonas fluorescens N1B4 was selected for mutant fitness profiling of a DNA-barcoded mutant library; with this approach four genes of importance were identified and the effects on interactions were evaluated with deletion mutants and mass spectrometry based metabolomics.

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Section: Introductionmentioning

confidence: 99%

Biofilm Interaction Mapping and Analysis (BIMA) of Interspecific Interactions in Pseudomonas Co-culture Biofilms

et al. 2021

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“…Pseudomonas stutzeri, a model denitrifying pseudomonas, can grow in diverse conditions (13) and its use has been demonstrated in a number of bioremediation processes, including phenol (14), carbon tetrachloride (15), uranium (16), polycyclic aromatic hydrocarbons (17) and diesel oil (18). Additionally, P. stutzeri has been discovered and used in a number of consortia based applications, such as bioremediation (19)(20)(21), wastewater denitrification (22,23), aquaculture water quality management (24,25), as a plant growth promoter for the biocontrol of phytopathogens (26,27) and manufacturing/municipal waste management (28,29). The use of P. stutzeri biofilms for various bioremediation efforts has potential benefits in terms of activity and yields for copper removal (30), drinking water denitrification (31) and naphthalene (32) and phenol (33) degradation.…”

Section: Introductionmentioning

confidence: 99%

Biofilm Interaction Mapping and Analysis (BIMA): A tool for deconstructing interspecific interactions in co-culture biofilms

Kosina

Rademacher

Wetmore

et al. 2021

Preprint

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Pseudomonas species are ubiquitous in nature and include numerous medically, agriculturally and technologically beneficial strains of which the interspecific interactions are of great interest for biotechnologies. Specifically, co-cultures containing Pseudomonas stutzeri have been used for bioremediation, biocontrol, aquaculture management and wastewater denitrification. Furthermore, the use of P. stutzeri biofilms, in combination with consortia based approaches, may offer advantages for these processes. Understanding the interspecific interaction within biofilm co-cultures or consortia provides a means for improvement of current technologies. However, the investigation of biofilm based consortia has been limited. We present an adaptable and scalable method for the analysis of macroscopic interactions (colony morphology, inhibition and invasion) between colony forming bacterial strains using an automated printing method followed by analysis of the genes and metabolites involved in the interactions. Using Biofilm Interaction Mapping and Analysis (BIMA), these interactions were investigated between P. stutzeri strain RCH2, a denitrifier isolated from chromium (VI) contaminated soil, and thirteen other species of pseudomonas isolated from non-contaminated soil. The metabolites and genes associated with both active co-culture growth and inhibitory growth were investigated using mass spectrometry based metabolomics and mutant fitness profiling of a DNA-barcoded mutant library. One interaction partner, Pseudomonas fluorescens N1B4 was selected for mutant fitness profiling; with this approach four genes of importance were identified and the effects on interactions were evaluated with deletion mutants and metabolomics.

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“…Several types of bacteria have been shown to function as aerobic denitrifying bacteria, including Pseudomonas sp. [1,4,5] , Acinetobacter sp. [3,6,7] , Paracoccus sp.…”

Section: Introductionmentioning

confidence: 99%

Optimal growth conditions and nutrient degradation characteristics of an aerobic denitrifying bacterium

Sun¹,

Shi²

2018

Preprint

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An efficient aerobic denitrifying bacterium, QX1, identified as Arthrobacter sp. by morphological observations, physiological-biochemical tests, and 16s rDNA sequence analysis, was isolated from burdock fields. A phylogenetic tree for this strain was built based on the 16s rDNA sequence, and the effects of nitrogen source, carbon source, pH value, and temperature on the growth of the strain, in addition to its denitrification characteristics, were evaluated. The results showed that under conditions of a gas bath with shaking at 160 rpm, suitable growth conditions for this strain were LB medium plus 1% acetic acid sodium, 30°C, and pH 8. With these conditions, the strain grew to the logarithmic phase after about 4 h, the logarithmic phase was maintained for 16 h, steady growth was observed for 24 h, and growth began to decline thereafter. QX1 exhibited alkali resistance and could tolerate low (15°C) and high temperatures (40°C). At 30°C, pH 8, and shaking at 160 rpm, strains could degrade nitrate, with a removal rate of 89.92% within 48 h in synthetic sewage.Thus, strain QX1 could have applications as an alkaline-tolerant, broad-spectrum temperature-tolerant, efficient denitrifying bacterium in the field of denitrification repair.

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Survival of the Aerobic DenitrifierPseudomonas stutzeriStrain TR2 during Co-Culture with Activated Sludge under Denitrifying Conditions

Cited by 11 publications

References 36 publications

Biofilm Interaction Mapping and Analysis (BIMA) of Interspecific Interactions in Pseudomonas Co-culture Biofilms

Biofilm Interaction Mapping and Analysis (BIMA) of Interspecific Interactions in Pseudomonas Co-culture Biofilms

Biofilm Interaction Mapping and Analysis (BIMA): A tool for deconstructing interspecific interactions in co-culture biofilms

Optimal growth conditions and nutrient degradation characteristics of an aerobic denitrifying bacterium

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